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Cited by 246

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Site-specific integrase-mediated transgenesis in mice via pronuclear injection

Tasic, Bosiljka, Hippenmeyer, Simon, Wang, Charlene, Gamboa, Matthew, Zong, Hui, Chen-Tsai, Yanru, Luo, Liqun·2011·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1019507108
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Abstract

Microinjection of recombinant DNA into zygotic pronuclei has been widely used for producing transgenic mice. However, with this method, the insertion site, integrity, and copy number of the transgene cannot be controlled. Here, we present an integrase-based approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy transgene can be inserted into predetermined chromosomal loci with high efficiency (up to 40%), and faithfully transmitted through generations. We show that neighboring transgenic elements and bacterial DNA within the transgene cause profound silencing and expression variability of the transgenic marker. Removal of these undesirable elements leads to global high-level marker expression from transgenes driven by a ubiquitous promoter. We also obtained faithful marker expression from a tissue-specific promoter. The technique presented here will greatly facilitate murine transgenesis and precise structure/function dissection of mammalian gene function and regulation in vivo.

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Crossref · 10.1073/pnas.1019507108

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2 of 2 references verified0 citation errors · 0 not checkable

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  • Using TARGATT homologous recombination technology, a single copy of the oATP8 construct was inserted via ΦC31 integrase and attPx3 docking sites into the mouse ROSA26 locus on chromosome 6 in the C57BL/6J(mtC57BL/6J) strain.

    “Using TARGATT homologous recombination technology,30 we inserted a single copy of the oATP8 construct via ΦC31 integrase and attPx3 docking sites into the mouse ROSA26 locus on chromosome 6 in the C57BL/6J(mtC57BL/6J) strain (Figure 1B).”

    From Exogenous expression of ATP8, a mitochondrial encoded protein, from the nucleus in vivo (2024) · cites this paper as [30]

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Research works printed but not linked: 45

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Metadata

Authors
Tasic, B., Hippenmeyer, S., Wang, C., Gamboa, M., Zong, H., Chen-Tsai, Y., & Luo, L.
Affiliations
  • The Howard Hughes Medical Institute and Department of Biology, Stanford University, Stanford, CA 94305;
  • Transgenic Facility, Stanford Cancer Center, Stanford University School of Medicine, Stanford, CA 94305; and
  • Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2011-04-04
Volume
108
Issue
19
Pages
7902-7907
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.1019507108
Cited by
246
References
2
Language
en

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