The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading
Open accessAbstract
Cell migration and spreading involve the coordination of membrane trafficking, actomyosin contraction, and modifications to plasma membrane tension and area. The biochemical or biophysical basis for this coordination is however unknown. In this study, we show that during cell spreading, lamellipodia protrusion flattens plasma membrane folds and blebs and, once the plasma membrane area is depleted, there is a temporary increase in membrane tension by over twofold that is followed by activation of exocytosis and myosin contraction. Further, an artificial increase in plasma membrane tension stopped lamellipodia protrusion and activated an exocytotic burst. Subsequent decrease in tension restored spreading with activation of contraction. Conversely, blebbistatin inhibition of actomyosin contraction resulted in an even greater increase in plasma membrane tension and exocytosis activation. This spatiotemporal synchronization indicates that membrane tension is the signal that coordinates membrane trafficking, actomyosin contraction, and plasma membrane area change. We suggest that cells use plasma membrane tension as a global physical parameter to control cell motility.
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Cites
Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae
Sinha, B., Köster, D., Ruez, R., Gonnord, P., Bastiani, M., Abankwa, D., Stan, R. V., Butler-Browne, G., Vedie, B., Johannes, L., et al. (2011) · Cell · doi:10.1016/j.cell.2010.12.031 · Cited by 969
Mechanism of shape determination in motile cells
Keren, K., Pincus, Z., Allen, G. M., Barnhart, E. L., Marriott, G., Mogilner, A., & Theriot, J. A. (2008) · Nature · doi:10.1038/nature06952 · Cited by 719
Periodic Lamellipodial Contractions Correlate with Rearward Actin Waves
Giannone, G., Dubin-Thaler, B. J., Döbereiner, H.-G., Kieffer, N., Bresnick, A. R., & Sheetz, M. P. (2004) · Cell · doi:10.1016/s0092-8674(04)00058-3 · Cited by 583
Self-citationLamellipodial Actin Mechanically Links Myosin Activity with Adhesion-Site Formation
Giannone, G., Dubin-Thaler, B. J., Rossier, O., Cai, Y., Chaga, O., Jiang, G., Beaver, W., Döbereiner, H.-G., Freund, Y., Borisy, G., et al. (2007) · Cell · doi:10.1016/j.cell.2006.12.039 · Cited by 509
Self-citationCell control by membrane–cytoskeleton adhesion
Sheetz, M. P. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35073095 · Cited by 476
Self-citationCell Surface Area Regulation and Membrane Tension
Morris, C. E., & Homann, U. (2001) · The Journal of Membrane Biology · doi:10.1007/s002320010040 · Cited by 471
Cell Spreading and Lamellipodial Extension Rate Is Regulated by Membrane Tension
Raucher, D., & Sheetz, M. P. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.148.1.127 · Cited by 455
Self-citationDeformation and flow of membrane into tethers extracted from neuronal growth cones
Hochmuth, F. M., Shao, J. Y., Dai, J., & Sheetz, M. P. (1996) · Biophysical Journal · doi:10.1016/s0006-3495(96)79577-2 · Cited by 378
Self-citationDirect measurement of the lamellipodial protrusive force in a migrating cell
Prass, M., Jacobson, K., Mogilner, A., & Radmacher, M. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200601159 · Cited by 346
Endocytic recycling pathways: emerging regulators of cell migration
Jones, M. C., Caswell, P. T., & Norman, J. C. (2006) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2006.08.003 · Cited by 283
Cited by
Actin Dynamics, Architecture, and Mechanics in Cell Motility
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C., & Plastino, J. (2014) · Physiological Reviews · doi:10.1152/physrev.00018.2013 · Cited by 1,457
Appreciating force and shape—the rise of mechanotransduction in cell biology
Iskratsch, T., Wolfenson, H., & Sheetz, M. P. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3903 · Cited by 798
Self-citationA fluorescent membrane tension probe
Colom, A., Derivery, E., Soleimanpour, S., Tomba, C., Molin, M. D., Sakai, N., González-Gaitán, M., Matile, S., & Roux, A. (2018) · Nature Chemistry · doi:10.1038/s41557-018-0127-3 · Cited by 572
Cell-cell communication: New insights and clinical implications
Su, J., Song, Y., Zhu, Z., Huang, X., Fan, J., Qiao, J., & Mao, F. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01888-z · Cited by 433
Shi, Z., Graber, Z. T., Baumgart, T., Stone, H. A., & Cohen, A. E. (2018) · Cell · doi:10.1016/j.cell.2018.09.054 · Cited by 355
Metadata
- Authors
- Gauthier, N. C., Fardin, M. A., Roca-Cusachs, P., & Sheetz, M. P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-08
- Volume
- 108
- Issue
- 35
- Pages
- 14467-14472
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2023922465
- Cited by
- 395
- References
- 24
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- green
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- Gauthier, N. C., Fardin, M. A., Roca-Cusachs, P., & Sheetz, M. P. (2011). Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1105845108
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