The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Quantitative assessment of Plasmodium falciparum sexual development reveals potent transmission-blocking activity by methylene blue
Open accessAbstract
Clinical studies and mathematical models predict that, to achieve malaria elimination, combination therapies will need to incorporate drugs that block the transmission of Plasmodium falciparum sexual stage parasites to mosquito vectors. Efforts to measure the activity of existing antimalarials on intraerythrocytic sexual stage gametocytes and identify transmission-blocking agents have, until now, been hindered by a lack of quantitative assays. Here, we report an experimental system using P. falciparum lines that stably express gametocyte-specific GFP-luciferase reporters, which enable the assessment of dose- and time-dependent drug action on gametocyte maturation and transmission. These studies reveal activity of the first-line antimalarial dihydroartemisinin and the partner drugs lumefantrine and pyronaridine against early gametocyte stages, along with moderate inhibition of mature gametocyte transmission to Anopheles mosquitoes. The other partner agents monodesethyl-amodiaquine and piperaquine showed activity only against immature gametocytes. Our data also identify methylene blue as a potent inhibitor of gametocyte development across all stages. This thiazine dye almost fully abolishes P. falciparum transmission to mosquitoes at concentrations readily achievable in humans, highlighting the potential of this chemical class to reduce the spread of malaria.
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Cites
Goodman & Gilman's The Pharmacological Basis of Therapeutics
Brunton, L. L. (1985) · Cited by 7,321
The Transcriptome of the Intraerythrocytic Developmental Cycle of Plasmodium falciparum
Bozdech, Z., Llinás, M., Pulliam, B. L., Wong, E. D., Zhu, J., & DeRisi, J. L. (2003) · PLoS Biology · doi:10.1371/journal.pbio.0000005 · Cited by 1,728
Bousema, T., & Drakeley, C. (2011) · Clinical Microbiology Reviews · doi:10.1128/cmr.00051-10 · Cited by 746
A Research Agenda to Underpin Malaria Eradication
Alonso, P. L., Brown, G., Arevalo-Herrera, M., Binka, F., Chitnis, C., Collins, F., Doumbo, O. K., Greenwood, B., Hall, B. F., Levine, M. M., et al. (2011) · PLoS Medicine · doi:10.1371/journal.pmed.1000406 · Cited by 667
Ruwende, C., Khoo, S. C., Snow, R. W., Yates, S. N. R., Kwiatkowski, D., Gupta, S., Warn, P., Allsopp, C. E. M., Gilbert, S. C., Peschu, N., et al. (1995) · Nature · doi:10.1038/376246a0 · Cited by 595
Artemisinin-based combination therapies: a vital tool in efforts to eliminate malaria
Eastman, R. T., & Fidock, D. A. (2009) · Nature Reviews Microbiology · doi:10.1038/nrmicro2239 · Cited by 568
Self-citationMaier, A. G., Rug, M., O'Neill, M. T., Brown, M., Chakravorty, S., Szestak, T., Chesson, J., Wu, Y., Hughes, K., Coppel, R. L., et al. (2008) · Cell · doi:10.1016/j.cell.2008.04.051 · Cited by 517
Effects of artemisinin derivatives on malaria transmissibility
Price, R. N., Nosten, F., Luxemburger, C., ter Kuile, F. O., Paiphun, L., Price, R. N., Luxemburger, C., Chongsuphajaisiddhi, T., White, N. J., Price, R. N., et al. (1996) · The Lancet · doi:10.1016/s0140-6736(96)91488-9 · Cited by 462
“Lest we forget you — methylene blue …”
Schirmer, R. H., Adler, H., Pickhardt, M., & Mandelkow, E. (2011) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2010.12.012 · Cited by 414
Primaquine revisited six decades after its discovery
Vale, N., Moreira, R., & Gomes, P. (2009) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2008.08.011 · Cited by 367
Cited by
Artemisinin Action and Resistance in Plasmodium falciparum
Tilley, L., Straimer, J., Gnädig, N. F., Ralph, S. A., & Fidock, D. A. (2016) · Trends in Parasitology · doi:10.1016/j.pt.2016.05.010 · Cited by 359
Self-citationLarsen, D. A., Bennett, A., Silumbe, K., Hamainza, B., Yukich, J. O., Keating, J., Littrell, M., Miller, J. M., Steketee, R. W., & Eisele, T. P. (2015) · The American Society of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.14-0347 · Cited by 108
Urban, P., & Fernandez-Busquets, X. (2014) · Current Medicinal Chemistry · doi:10.2174/09298673113206660292 · Cited by 39
Pathak, A. K., Shiau, J. C., Thomas, M. B., & Murdock, C. C. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02651 · Cited by 35
Metadata
- Authors
- Adjalley, S. H., Johnston, G. L., Li, T., Eastman, R. T., Ekland, E. H., Eappen, A. G., Richman, A., Sim, B. K. L., Lee, M. C. S., Hoffman, S. L., & Fidock, D. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-10-31
- Volume
- 108
- Issue
- 47
- Pages
- E1214-23
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2101792514
- Cited by
- 309
- References
- 45
- FWCI
- 22.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Adjalley, S. H., Johnston, G. L., Li, T., Eastman, R. T., Ekland, E. H., Eappen, A. G., Richman, A., Sim, B. K. L., Lee, M. C. S., Hoffman, S. L., & Fidock, D. A. (2011). Quantitative assessment of Plasmodium falciparum sexual development reveals potent transmission-blocking activity by methylene blue. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1112037108
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