The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
MicroRNA regulation of plant innate immune receptors
Open accessAbstract
Plant genomes contain large numbers of cell surface leucine-rich repeat (LRR) and intracellular nucleotide binding (NB)-LRR immune receptors encoded by resistance ( R ) genes that recognize specific pathogen effectors and trigger resistance responses. The unregulated expression of NB-LRR genes can trigger autoimmunity in the absence of pathogen infection and inhibit plant growth. Despite the potential serious consequence on agricultural production, the mechanisms regulating R -gene expression are not well understood. We identified microRNA (miRNA) progenitor genes precursor transcripts, and two miRNAs [nta-miR6019 (22-nt) and nta-miR6020 (21-nt)] that guide cleavage of transcripts of the Toll and Interleukin-1 receptor-NB-LRR immune receptor N from tobacco that confers resistance to tobacco mosaic virus (TMV). We further showed that cleavage by nta-miR6019 triggers RNA-dependent RNA polymerase 6- and ribonuclease Dicer-like 4-dependent biogenesis of 21-nt secondary siRNAs “in phase” with the 22-nt miR6019 cleavage site. Furthermore, we found that processing of the 22-nt nta-miR6019 depended on an asymmetric bulge caused by mismatch in the nta-miR6019 precursor. Interestingly, coexpression of N with nta-miR6019 and nta-miR6020 resulted in attenuation of N -mediated resistance to TMV, indicating that these miRNAs have functional roles in NB-LRR regulation. Using a bioinformatics approach, we identified six additional 22-nt miRNA and two 21-nt miRNA families from three Solanaceae species—tobacco, tomato, and potato. We show that members of these miRNA families cleave transcripts of predicted functional R genes and trigger production of phased secondary 21-nt siRNAs. Our results demonstrate a conserved role for miRNAs and secondary siRNAs in NB-LRR/LRR immune receptor gene regulation and pathogen resistance in Solanaceae .
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Signaling in Plant-Microbe Interactions
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Cited by
A Truncated Singleton NLR Causes Hybrid Necrosis in Arabidopsis thaliana
Barragan, A. C., Collenberg, M., Wang, J., Lee, R. R. Q., Cher, W. Y., Rabanal, F. A., Ashkenazy, H., Weigel, D., & Chae, E. (2021) · Molecular Biology and Evolution · doi:10.1093/molbev/msaa245 · Cited by 46
Metadata
- Authors
- Li, F., Pignatta, D., Bendix, C., Brunkard, J. O., Cohn, M. M., Tung, J., Sun, H., Kumar, P., & Baker, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-01-18
- Volume
- 109
- Issue
- 5
- Pages
- 1790-1795
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2096194109
- Cited by
- 616
- References
- 30
- FWCI
- 54.93
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Li, F., Pignatta, D., Bendix, C., Brunkard, J. O., Cohn, M. M., Tung, J., Sun, H., Kumar, P., & Baker, B. (2012). MicroRNA regulation of plant innate immune receptors. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1118282109
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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