The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG
Open accessAbstract
We describe a method for light-inducible and tissue-selective cell ablation using a genetically encoded photosensitizer, miniSOG (mini singlet oxygen generator). miniSOG is a newly engineered fluorescent protein of 106 amino acids that generates singlet oxygen in quantum yield upon blue-light illumination. We transgenically expressed mitochondrially targeted miniSOG (mito-miniSOG) in Caenorhabditis elegans neurons. Upon blue-light illumination, mito-miniSOG causes rapid and effective death of neurons in a cell-autonomous manner without detectable damages to surrounding tissues. Neuronal death induced by mito-miniSOG appears to be independent of the caspase CED-3, but the clearance of the damaged cells partially depends on the phagocytic receptor CED-1, a homolog of human CD91. We show that neurons can be killed at different developmental stages. We further use this method to investigate the role of the premotor interneurons in regulating the convulsive behavior caused by a gain-of-function mutation in the neuronal acetylcholine receptor acr-2. Our findings support an instructive role for the interneuron AVB in controlling motor neuron activity and reveal an inhibitory effect of the backward premotor interneurons on the forward interneurons. In summary, the simple inducible cell ablation method reported here allows temporal and spatial control and will prove to be a useful tool in studying the function of specific cells within complex cellular contexts.
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Cites
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Photodynamic therapy of cancer: An update
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Faumont, S., Rondeau, G., Thiele, T. R., Lawton, K. J., McCormick, K. E., Sottile, M., Griesbeck, O., Heckscher, E. S., Roberts, W. M., Doe, C. Q., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024666 · Cited by 128
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Zhou, Z., Song, J., Nie, L., & Chen, X. (2016) · Chemical Society Reviews · doi:10.1039/c6cs00271d · Cited by 2,098
Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy
Martell, J. D., Deerinck, T. J., Sancak, Y., Poulos, T. L., Mootha, V. K., Sosinsky, G. E., Ellisman, M. H., & Ting, A. Y. (2012) · Nature Biotechnology · doi:10.1038/nbt.2375 · Cited by 722
Very long-term memories may be stored in the pattern of holes in the perineuronal net
Tsien, R. Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1310158110 · Cited by 244
Self-citationDistributed rhythm generators underlie Caenorhabditis elegans forward locomotion
Fouad, A. D., Teng, S., Mark, J. R., Liu, A., Alvarez-Illera, P., Ji, H., Du, A., Bhirgoo, P. D., Cornblath, E., Guan, S. A., et al. (2018) · eLife · doi:10.7554/elife.29913 · Cited by 79
Synaptic polarity of the interneuron circuit controlling C. elegans locomotion
Rakowski, F., Srinivasan, J., Sternberg, P. W., & Karbowski, J. (2013) · Frontiers in Computational Neuroscience · doi:10.3389/fncom.2013.00128 · Cited by 41
Metadata
- Authors
- Qi, Y. B., Garren, E. J., Shu, X., Tsien, R. Y., & Jin, Y.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-04-24
- Volume
- 109
- Issue
- 19
- Pages
- 7499-7504
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2042630054
- Cited by
- 200
- References
- 5
- FWCI
- 7.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Qi, Y. B., Garren, E. J., Shu, X., Tsien, R. Y., & Jin, Y. (2012). Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1204096109
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