The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Fighting malaria with engineered symbiotic bacteria from vector mosquitoes
Open accessAbstract
The most vulnerable stages of Plasmodium development occur in the lumen of the mosquito midgut, a compartment shared with symbiotic bacteria. Here, we describe a strategy that uses symbiotic bacteria to deliver antimalaria effector molecules to the midgut lumen, thus rendering host mosquitoes refractory to malaria infection. The Escherichia coli hemolysin A secretion system was used to promote the secretion of a variety of anti- Plasmodium effector proteins by Pantoea agglomerans , a common mosquito symbiotic bacterium. These engineered P. agglomerans strains inhibited development of the human malaria parasite Plasmodium falciparum and rodent malaria parasite Plasmodium berghei by up to 98%. Significantly, the proportion of mosquitoes carrying parasites (prevalence) decreased by up to 84% for two of the effector molecules, scorpine, a potent antiplasmodial peptide and (EPIP) 4 , four copies of Plasmodium enolase–plasminogen interaction peptide that prevents plasminogen binding to the ookinete surface. We demonstrate the use of an engineered symbiotic bacterium to interfere with the development of P. falciparum in the mosquito. These findings provide the foundation for the use of genetically modified symbiotic bacteria as a powerful tool to combat malaria.
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Cites
Global malaria mortality between 1980 and 2010: a systematic analysis
Murray, C. J., Rosenfeld, L. C., Lim, S. S., Andrews, K. G., Foreman, K. J., Haring, D., Fullman, N., Naghavi, M., Lozano, R., & Lopez, A. D. (2012) · The Lancet · doi:10.1016/s0140-6736(12)60034-8 · Cited by 1,452
Malaria: progress, perils, and prospects for eradication
Greenwood, B. M., Fidock, D. A., Kyle, D. E., Kappe, S. H. I., Alonso, P. L., Collins, F. H., & Duffy, P. E. (2008) · Journal of Clinical Investigation · doi:10.1172/jci33996 · Cited by 709
The Ecology and Biogeography of Microorganisms on Plant Surfaces
Andrews, J. H., & Harris, R. F. (2000) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.38.1.145 · Cited by 648
Dynamic Gut Microbiome across Life History of the Malaria Mosquito Anopheles gambiae in Kenya
Wang, Y., Gilbreath, T. M., Kukutla, P., Yan, G., & Xu, J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024767 · Cited by 584
Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite
Ito, J., Ghosh, A., Moreira, L. A., Wimmer, E. A., & Jacobs-Lorena, M. (2002) · Nature · doi:10.1038/417452a · Cited by 572
Self-citationMicrobiome influences on insect host vector competence
Weiss, B., & Aksoy, S. (2011) · Trends in Parasitology · doi:10.1016/j.pt.2011.05.001 · Cited by 393
A synthetic homing endonuclease-based gene drive system in the human malaria mosquito
Windbichler, N., Menichelli, M., Papathanos, P. A., Thyme, S. B., Li, H., Ulge, U. Y., Hovde, B. T., Baker, D., Monnat, R. J., Burt, A., et al. (2011) · Nature · doi:10.1038/nature09937 · Cited by 360
Trape, J.-F., Tall, A., Diagne, N., Ndiath, O., Ly, A. B., Faye, J., Dieye-Ba, F., Roucher, C., Bouganali, C., Badiane, A., et al. (2011) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(11)70194-3 · Cited by 328
A Synthetic Maternal-Effect Selfish Genetic Element Drives Population Replacement in Drosophila
Chen, C.-H., Huang, H., Ward, C. M., Su, J. T., Schaeffer, L. V., Guo, M., & Hay, B. A. (2007) · Science · doi:10.1126/science.1138595 · Cited by 318
Scorpine, an anti‐malaria and anti‐bacterial agent purified from scorpion venom
Conde, R., Zamudio, F. Z., Rodrı́guez, M. H., & Possani, L. D. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01384-3 · Cited by 290
Cited by
Phillips, M. A., Burrows, J. N., Manyando, C., van Huijsduijnen, R. H., Van Voorhis, W. C., & Wells, T. N. C. (2017) · Nature Reviews Disease Primers · doi:10.1038/nrdp.2017.50 · Cited by 649
Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality
Wei, G., Lai, Y., Wang, G., Chen, H., Li, F., & Wang, S. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1703546114 · Cited by 389
Self-citation
Metadata
- Authors
- Wang, S., Ghosh, A. K., Bongio, N., Stebbings, K. A., Lampe, D. J., & Jacobs-Lorena, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-07-16
- Volume
- 109
- Issue
- 31
- Pages
- 12734-12739
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2039411469
- Cited by
- 318
- References
- 28
- FWCI
- 20.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, S., Ghosh, A. K., Bongio, N., Stebbings, K. A., Lampe, D. J., & Jacobs-Lorena, M. (2012). Fighting malaria with engineered symbiotic bacteria from vector mosquitoes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1204158109
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
