The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Direct activation of full-length proapoptotic BAK
Open accessAbstract
Proapoptotic B-cell lymphoma 2 (BCL-2) antagonist/killer (BAK) and BCL-2–associated X (BAX) form toxic mitochondrial pores in response to cellular stress. Whereas BAX resides predominantly in the cytosol, BAK is constitutively localized to the outer mitochondrial membrane. Select BCL-2 homology domain 3 (BH3) helices activate BAX directly by engaging an α1/α6 trigger site. The inability to express full-length BAK has hampered full dissection of its activation mechanism. Here, we report the production of full-length, monomeric BAK by mutagenesis-based solubilization of its C-terminal α-helical surface. Recombinant BAK autotranslocates to mitochondria but only releases cytochrome c upon BH3 triggering. A direct activation mechanism was explicitly demonstrated using a liposomal system that recapitulates BAK-mediated release upon addition of BH3 ligands. Photoreactive BH3 helices mapped both triggering and autointeractions to the canonical BH3-binding pocket of BAK, whereas the same ligands crosslinked to the α1/α6 site of BAX. Thus, activation of both BAK and BAX is initiated by direct BH3–interaction but at distinct trigger sites. These structural and biochemical insights provide opportunities for developing proapoptotic agents that activate the death pathway through direct but differential engagement of BAK and BAX.
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Self-citation
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Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy
Czabotar, P. E., Lessene, G., Strasser, A., & Adams, J. M. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3722 · Cited by 3,219
Regulation of apoptosis in health and disease: the balancing act of BCL-2 family proteins
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Bax, Bak and beyond — mitochondrial performance in apoptosis
Peña‐Blanco, A., & García‐Sáez, A. J. (2018) · The FEBS Journal · doi:10.1111/febs.14186 · Cited by 930
The mystery of BCL2 family: Bcl-2 proteins and apoptosis: an update
Siddiqui, W. A., Ahad, A., & Ahsan, H. (2015) · Archives of Toxicology · doi:10.1007/s00204-014-1448-7 · Cited by 696
BCL-2 proteins and apoptosis: Recent insights and unknowns
Edlich, F. (2018) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2017.06.190 · Cited by 632
Metadata
- Authors
- Leshchiner, E. S., Braun, C. R., Bird, G. H., & Walensky, L. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-02-12
- Volume
- 110
- Issue
- 11
- Pages
- E986-95
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2167182598
- Cited by
- 161
- References
- 12
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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