The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells
Open accessAbstract
Inducible degradation is a powerful approach for identifying the function of a specific protein or protein complex. Recently, a plant auxin-inducible degron (AID) system has been shown to degrade AID-tagged target proteins in nonplant cells. Here, we demonstrate that an AID-tagged protein can functionally replace an endogenous protein depleted by RNAi, leading to an inducible null phenotype rapidly after auxin addition. The AID system is shown to be capable of controlling the stability of AID-tagged proteins that are in either nuclear or cytoplasmic compartments and even when incorporated into protein complexes. Induced degradation occurs rapidly after addition of auxin with protein half-life reduced to as little as 9 min and proceeding to completion with first-order kinetics. AID-mediated instability is demonstrated to be rapidly reversible. Induced degradation is shown to initiate and continue in all cell cycle phases, including mitosis, making this system especially useful for identifying the function(s) of proteins of interest during specific points in the mammalian cell cycle.
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Cites
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Cited by
Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors
Natsume, T., Kiyomitsu, T., Saga, Y., & Kanemaki, M. T. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.03.001 · Cited by 673
A pathway for mitotic chromosome formation
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A Method for the Acute and Rapid Degradation of Endogenous Proteins
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Chemically induced proximity in biology and medicine
Stanton, B. Z., Chory, E. J., & Crabtree, G. R. (2018) · Science · doi:10.1126/science.aao5902 · Cited by 475
Metadata
- Authors
- Holland, A. J., Fachinetti, D., Han, J. S., & Cleveland, D. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-11-12
- Volume
- 109
- Issue
- 49
- Pages
- E3350-7
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2082001960
- Cited by
- 303
- References
- 8
- FWCI
- 3.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Holland, A. J., Fachinetti, D., Han, J. S., & Cleveland, D. W. (2012). Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1216880109
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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