A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila
Open accessAbstract
Innate behaviors are often executed in concert with accompanying physiological programs. How this coordination is achieved is poorly understood. Mating behavior and the transfer of sperm and seminal fluid (SSFT) provide a model for understanding how concerted behavioral and physiological programs are coordinated. Here we identify a male-specific neural pathway that coordinates the timing of SSFT with the duration of copulation behavior in Drosophila. Silencing four abdominal ganglion (AG) interneurons (INs) that contain the neuropeptide corazonin (Crz) both blocked SSFT and substantially lengthened copulation duration. Activating these Crz INs caused rapid ejaculation in isolated males, a phenotype mimicked by injection of Crz peptide. Crz promotes SSFT by activating serotonergic (5-HT) projection neurons (PNs) that innervate the accessory glands. Activation of these PNs in copulo caused premature SSFT and also shortened copulation duration. However, mating terminated normally when these PNs were silenced, indicating that SSFT is not required for appropriate copulation duration. Thus, the lengthened copulation duration phenotype caused by silencing Crz INs is independent of the block to SSFT. We conclude that four Crz INs independently control SSFT and copulation duration, thereby coupling the timing of these two processes.
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CRZ signaling coordinates aspects of copulation and fecundity in Drosophila and steroid synthesis in Octopus gonads.
“For instance, CRZ signaling coordinates aspects of copulation and fecundity in Drosophila (29, 30) and steroid synthesis in Octopus gonads (31).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [29]
In insects, CRZ signaling regulates reproduction (sperm transfer).
“and reproduction (sperm transfer) (29, 81)”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [29]
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- Authors
- Tayler, T. D., Pacheco, D. A., Hergarden, A. C., Murthy, M., & Anderson, D. J.
- Affiliations
- Princeton University (US)
- California Institute of Technology (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-11-28
- Volume
- 109
- Issue
- 50
- Pages
- 20697-20702
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3528491
- OpenAlex
- W2150959561
- Cited by
- 150
- References
- 33
- Citations by year
- 2026: 11 · 2025: 23 · 2024: 13 · 2023: 6 · 2022: 11 · 2021: 16 · 2020: 15 · 2019: 12 · 2018: 9 · 2017: 3 · 2016: 13 · 2015: 7 · 2014: 5 · 2013: 6
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health (5t32ns007251-22, 5f32ns058132-02)
- National Institute on Drug Abuse (R01DA031389)
- National Institute of Neurological Disorders and Stroke (T32 NS-007251, NS007251, F32 NS058132, 5F32NS058132)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Animal Behavior and Reproduction
- Keywords
- sperm transfer, copulation duration, corazonin, neuropeptides, accessory glands, ejaculation, mating behavior
- MeSH terms
- Animals, Copulation, Drosophila melanogaster, Ejaculation, Female, Ganglia, Interneurons, Male, Models, Biological, Neuropeptides, Sperm Transport, Genes, Insect, Receptors, Neuropeptide, Drosophila Proteins, Animals, Genetically Modified
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