Cited by 150Neurobiology and Insect Physiology ResearchNeuroendocrine regulation and behaviorAnimal Behavior and Reproduction

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A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila

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Tayler, Timothy D., Pacheco, Diego A., Hergarden, Anne C., Murthy, Mala, Anderson, David J.·2012·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1218246109PMC PMC3528491

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Abstract

Innate behaviors are often executed in concert with accompanying physiological programs. How this coordination is achieved is poorly understood. Mating behavior and the transfer of sperm and seminal fluid (SSFT) provide a model for understanding how concerted behavioral and physiological programs are coordinated. Here we identify a male-specific neural pathway that coordinates the timing of SSFT with the duration of copulation behavior in Drosophila. Silencing four abdominal ganglion (AG) interneurons (INs) that contain the neuropeptide corazonin (Crz) both blocked SSFT and substantially lengthened copulation duration. Activating these Crz INs caused rapid ejaculation in isolated males, a phenotype mimicked by injection of Crz peptide. Crz promotes SSFT by activating serotonergic (5-HT) projection neurons (PNs) that innervate the accessory glands. Activation of these PNs in copulo caused premature SSFT and also shortened copulation duration. However, mating terminated normally when these PNs were silenced, indicating that SSFT is not required for appropriate copulation duration. Thus, the lengthened copulation duration phenotype caused by silencing Crz INs is independent of the block to SSFT. We conclude that four Crz INs independently control SSFT and copulation duration, thereby coupling the timing of these two processes.

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Authors: Tayler, T. D., Pacheco, D. A., Hergarden, A. C., Murthy, M., & Anderson, D. J.
Affiliations: Princeton University (US)
California Institute of Technology (US)
ORCID: Pacheco, Diego A.
Murthy, Mala
Anderson, David J.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2012-11-28
Volume: 109
Issue: 50
Pages: 20697-20702
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1218246109
PMC: PMC3528491
OpenAlex: W2150959561
Cited by: 150
References: 33
Citations by year: 2026: 11 · 2025: 23 · 2024: 13 · 2023: 6 · 2022: 11 · 2021: 16 · 2020: 15 · 2019: 12 · 2018: 9 · 2017: 3 · 2016: 13 · 2015: 7 · 2014: 5 · 2013: 6
FWCI: 2.37
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
CaltechAUTHORS (California Institute of Technology) (submittedVersion)
Funders: Howard Hughes Medical Institute
National Institutes of Health (5t32ns007251-22, 5f32ns058132-02)
National Institute on Drug Abuse (R01DA031389)
National Institute of Neurological Disorders and Stroke (T32 NS-007251, NS007251, F32 NS058132, 5F32NS058132)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Animal Behavior and Reproduction
Keywords: sperm transfer, copulation duration, corazonin, neuropeptides, accessory glands, ejaculation, mating behavior
MeSH terms: Animals, Copulation, Drosophila melanogaster, Ejaculation, Female, Ganglia, Interneurons, Male, Models, Biological, Neuropeptides, Sperm Transport, Genes, Insect, Receptors, Neuropeptide, Drosophila Proteins, Animals, Genetically Modified

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A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila

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