CoChat
Sign Up
Cited by 104Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsTreatment of Major Depression

Quantification of spread of cerebellar long-term depression with chemical two-photon uncaging of glutamate

Open access
Wang, Samuel S.-H., Khiroug, Leonard, Augustine, George J.·2000·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.130414597PMC PMC27000
Open this paper in CoChat — free account

Abstract

Localized, chemical two-photon photolysis of caged glutamate was used to map the changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptors caused by long-term synaptic depression (LTD) in cerebellar Purkinje cells. LTD produced by pairing parallel fiber activity with depolarization was accompanied by a decline in the response of Purkinje cells to uncaged glutamate that accounted for both the time course and magnitude of LTD. This depression of glutamate responses was observed not only at the site of parallel fiber stimulation but also at more distant sites. The amount of LTD decreased with distance and was half-maximal 50 microm away from the site of parallel fiber activity. Estimation of the number of parallel fibers active during LTD induction indicates that LTD modified glutamate receptors not only at active synapses but also at 600 times as many inactive synapses on a single Purkinje cell. Therefore, both active and inactive parallel fiber synapses can undergo changes at a postsynaptic locus as a result of associative pre- and postsynaptic activity.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.130414597

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 57

  1. [1]Sherrington C. The Integrative Action of the Nervous System. 2nd Ed. New Haven, CT: Yale Univ. Press; 1947. [Google Scholar]
  2. [2]Hebb D O. The Organization of Behavior: A Neuropsychological Theory. New York: Wiley; 1949. [Google Scholar]
  3. [3]Kandel E R, Schwartz J H. Science. 1982;218:433–443. doi: 10.1126/science.6289442. [DOI] [PubMed] [Google Scholar] · DOI
  4. [4]Vincent P, Marty A. Neuron. 1993;11:885–893. doi: 10.1016/0896-6273(93)90118-b. [DOI] [PubMed] [Google Scholar] · DOI
  5. [5]Schuman E M, Madison D V. Science. 1994;263:532–536. doi: 10.1126/science.8290963. [DOI] [PubMed] [Google Scholar] · DOI
  6. [6]McMahon L L, Kauer J A. Neuron. 1997;18:295–305. doi: 10.1016/s0896-6273(00)80269-x. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  7. [8]Fitzsimonds R M, Song H J, Poo M M. Nature (London) 1997;388:439–448. doi: 10.1038/41267. [DOI] [PubMed] [Google Scholar] · DOI
  8. [9]Ito M. Annu Rev Neurosci. 1989;12:85–102. doi: 10.1146/annurev.ne.12.030189.000505. [DOI] [PubMed] [Google Scholar] · DOI
  9. [10]Thompson R F. Phil Trans R Soc London. 1990;329:161–170. doi: 10.1098/rstb.1990.0161. [DOI] [PubMed] [Google Scholar] · DOI
  10. [11]Mauk M D, Garcia K S, Medina J F, Steele P M. Neuron. 1998;20:359–362. doi: 10.1016/s0896-6273(00)80978-2. [DOI] [PubMed] [Google Scholar] · DOI
  11. [12]Lisberger S G. Cell. 1998;92:701–704. doi: 10.1016/s0092-8674(00)81397-5. [DOI] [PubMed] [Google Scholar] · DOI
  12. [13]Ito M, Sakurai M, Tongroach P. J Physiol (London) 1982;324:113–134. doi: 10.1113/jphysiol.1982.sp014103. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  13. [14]Sakurai M. J Physiol (London) 1987;394:463–480. doi: 10.1113/jphysiol.1987.sp016881. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  14. [15]Linden D J, Connor J A. Annu Rev Neurosci. 1995;18:319–357. doi: 10.1146/annurev.ne.18.030195.001535. [DOI] [PubMed] [Google Scholar] · DOI
  15. [16]Ekerot C F, Kano M. Brain Res. 1985;342:357–360. doi: 10.1016/0006-8993(85)91136-9. [DOI] [PubMed] [Google Scholar] · DOI
  16. [17]Kano M, Kato M. Nature (London) 1987;325:276–279. doi: 10.1038/325276a0. [DOI] [PubMed] [Google Scholar] · DOI
  17. [18]Hartell N. Neuron. 1996;16:601–610. doi: 10.1016/s0896-6273(00)80079-3. [DOI] [PubMed] [Google Scholar] · DOI
  18. [19]Linden D J, Dickinson M H, Smeyne M, Connor J A. Neuron. 1991;7:81–89. doi: 10.1016/0896-6273(91)90076-c. [DOI] [PubMed] [Google Scholar] · DOI
  19. [20]Hemart N, Daniel H, Jaillard D, Crepel F. Neurosci Res. 1994;19:213–221. doi: 10.1016/0168-0102(94)90145-7. [DOI] [PubMed] [Google Scholar] · DOI
  20. [21]Pettit D L, Wang S S-H, Gee K R, Augustine G J. Neuron. 1997;19:465–471. doi: 10.1016/s0896-6273(00)80361-x. [DOI] [PubMed] [Google Scholar] · DOI
  21. [22]Edwards F A, Konnerth A, Sakmann B, Takahashi T. Pflügers Arch. 1989;414:600–612. doi: 10.1007/BF00580998. [DOI] [PubMed] [Google Scholar] · DOI
  22. [23]Doroshenko P A, Woppmann A, Miljanich G, Augustine G J. Neuropharmacology. 1997;36:865–872. doi: 10.1016/s0028-3908(97)00032-4. [DOI] [PubMed] [Google Scholar] · DOI
  23. [24]Wang S S-H, Augustine G J. Neuron. 1995;15:755–760. doi: 10.1016/0896-6273(95)90167-1. [DOI] [PubMed] [Google Scholar] · DOI
  24. [25]Eilers J-K, Augustine G J, Konnerth A. Nature (London) 1995;373:155–158. doi: 10.1038/373155a0. [DOI] [PubMed] [Google Scholar] · DOI
  25. [26]Finch E A, Augustine G J. Nature (London) 1998;396:753–756. doi: 10.1038/25541. [DOI] [PubMed] [Google Scholar] · DOI
  26. [27]Jolliffe I T. Principal Component Analysis. New York: Springer; 1986. [Google Scholar]
  27. [28]Mitra P P, Pesaran B. Biophys J. 1999;76:691–708. doi: 10.1016/S0006-3495(99)77236-X. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  28. [29]Kandler K, Katz L C, Kauer J A. Nat Neurosci. 1998;1:119–123. doi: 10.1038/368. [DOI] [PubMed] [Google Scholar] · DOI
  29. [30]Honore T, Drejer J, Fletcher E J, Jacobson P, Lodge D, Nielson F E. Science. 1988;241:701–703. doi: 10.1126/science.2899909. [DOI] [PubMed] [Google Scholar] · DOI
  30. [31]Perkel D J, Hestrin S, Sah P, Nicoll R A. Proc R Soc London B. 1990;241:116–121. doi: 10.1098/rspb.1990.0074. [DOI] [PubMed] [Google Scholar] · DOI
  31. [32]Konnerth A, Llano I, Armstrong C M. Proc Natl Acad Sci USA. 1990;87:2662–2665. doi: 10.1073/pnas.87.7.2662. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  32. [33]Palay S L, Chan-Palay V. Cerebellar Cortex. Berlin: Springer; 1974. [Google Scholar]
  33. [34]Pettit, D. L. & Augustine, G. J. (2000) J. Neurophysiol., in press. [DOI] [PubMed]
  34. [35]Takechi H, Eilers J, Konnerth A. Nature (London) 1998;396:757–760. doi: 10.1038/25547. [DOI] [PubMed] [Google Scholar] · DOI
  35. [36]Sakurai M. Proc Natl Acad Sci USA. 1990;87:3383–3385. doi: 10.1073/pnas.87.9.3383. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  36. [37]Crepel F, Jaillard D. J Physiol (London) 1991;432:123–141. doi: 10.1113/jphysiol.1991.sp018380. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  37. [38]Konnerth A, Dreessen J, Augustine G J. Proc Natl Acad Sci USA. 1992;89:7051–7055. doi: 10.1073/pnas.89.15.7051. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  38. [39]Karachot L, Kado R T, Ito M. Neurosci Res. 1994;21:161–168. doi: 10.1016/0168-0102(94)90158-9. [DOI] [PubMed] [Google Scholar] · DOI
  39. [40]Salin P A, Malenka R C, Nicoll R A. Neuron. 1996;16:797–803. doi: 10.1016/s0896-6273(00)80099-9. [DOI] [PubMed] [Google Scholar] · DOI
  40. [41]Napper R M A, Harvey R J. J Comp Neurol. 1988;274:158–167. doi: 10.1002/cne.902740203. [DOI] [PubMed] [Google Scholar] · DOI
  41. [42]Harris K M, Stevens J K. J Neurosci. 1988;8:4455–4469. doi: 10.1523/JNEUROSCI.08-12-04455.1988. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI · Paper page
  42. [43]Barbour B. Neuron. 1993;11:759–769. doi: 10.1016/0896-6273(93)90085-6. [DOI] [PubMed] [Google Scholar] · DOI
  43. [44]Lev-Ram V, Jiang T, Wood J, Lawrence D S, Tsien R Y. Neuron. 1997;18:1025–1038. doi: 10.1016/s0896-6273(00)80340-2. [DOI] [PubMed] [Google Scholar] · DOI
  44. [45]Berry M, Bradley P. Brain Res. 1976;112:1–35. doi: 10.1016/0006-8993(76)90331-0. [DOI] [PubMed] [Google Scholar] · DOI
  45. [46]Spruston N, Jaffe D B, Williams S H, Johnston D. J Neurophysiol. 1993;70:781–802. doi: 10.1152/jn.1993.70.2.781. [DOI] [PubMed] [Google Scholar] · DOI
  46. [47]Kullmann D M, Asztely F. Trends Neurosci. 1998;21:8–14. doi: 10.1016/s0166-2236(97)01150-8. [DOI] [PubMed] [Google Scholar] · DOI
  47. [48]Napper R M A, Harvey R J. Comp Neurol. 1988;274:168–177. doi: 10.1002/cne.902740204. [DOI] [PubMed] [Google Scholar] · DOI
  48. [49]Llinas R, Bloedel J R, Hillman D E. J Neurophysiol. 1969;32:847–870. doi: 10.1152/jn.1969.32.6.847. [DOI] [PubMed] [Google Scholar] · DOI
  49. [50]Hartell N A. J Neurosci. 1996;16:2881–2890. doi: 10.1523/JNEUROSCI.16-09-02881.1996. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  50. [51]Kasono K, Hirano T. NeuroReport. 1995;6:569–572. doi: 10.1097/00001756-199502000-00040. [DOI] [PubMed] [Google Scholar] · DOI
  51. [52]Khodakhah K, Armstrong C M. Proc Natl Acad Sci USA. 1997;94:14009–14114. doi: 10.1073/pnas.94.25.14009. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  52. [53]Häusser M. Soc Neurosci Abstr. 1994;20:891. [Google Scholar]
  53. [54]Malinski T, Taha Z, Grunfeld S, Patton S, Kapturczak M, Tomboulian P. Biochem Biophys Res Commun. 1993;193:1076–1082. doi: 10.1006/bbrc.1993.1735. [DOI] [PubMed] [Google Scholar] · DOI
  54. [55]Huang R-C, Gillette R. J Gen Physiol. 1991;98:835–848. doi: 10.1085/jgp.98.4.835. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  55. [56]Ito M, Karachot L. Neurosci Res. 1992;14:27–38. doi: 10.1016/s0168-0102(05)80004-5. [DOI] [PubMed] [Google Scholar] · DOI
  56. [57]Nakazawa K, Mikawa S, Hashikawa T, Ito M. Neuron. 1995;15:697–709. doi: 10.1016/0896-6273(95)90157-4. [DOI] [PubMed] [Google Scholar] · DOI
  57. [58]Boxall A R, Lancaster B, Garthwaite J. Neuron. 1996;16:805–813. doi: 10.1016/s0896-6273(00)80100-2. [DOI] [PubMed] [Google Scholar] · DOI

Cited by

Sorted by citations

Authors
Wang, S. S.-H., Khiroug, L., & Augustine, G. J.
Affiliations
  • Duke Medical Center (US)
  • Salk Institute for Biological Studies (US)
Corresponding author
Augustine, George J.
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2000-07-11
Volume
97
Issue
15
Pages
8635-8640
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.130414597
PMC
PMC27000
OpenAlex
W1986155820
Cited by
104
References
1
Citations by year
2025: 1 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 6 · 2014: 5 · 2013: 2 · 2012: 3
FWCI
4.01
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (R01NS034045, NS-09457, F32NS009457, NS34045)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
Keywords
long-term depression, Purkinje cells, caged glutamate, two-photon photolysis, AMPA receptors, parallel fiber synapses, synaptic plasticity, postsynaptic plasticity, associative plasticity, glutamate uncaging
MeSH terms
Animals, Cells, Cultured, Cerebellum, Glutamates, Purkinje Cells, Time Factors, Photons, Receptors, AMPA, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review