The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Single-strand interruptions in replicating chromosomes cause double-strand breaks
Open accessAbstract
Replication-dependent chromosomal breakage suggests that replication forks occasionally run into nicks in template DNA and collapse, generating double-strand ends. To model replication fork collapse in vivo , I constructed phage λ chromosomes carrying the nicking site of M13 bacteriophage and infected with these substrates Escherichia coli cells, producing M13 nicking enzyme. I detected double-strand breaks at the nicking sites in λ DNA purified from these cells. The double-strand breakage depends on ( i ) the presence of the nicking site; ( ii ) the production of the nicking enzyme; and ( iii ) replication of the nick-containing chromosome. Replication fork collapse at nicks in template DNA explains diverse phenomena, including eukaryotic cell killing by DNA topoisomerase inhibitors and inviability of recombination-deficient vertebrate cell lines.
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Bootsma, D., & Hoeijmakers, J. H. J. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)81408-9 · Cited by 2,282
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[17] A rapid alkaline extraction method for the isolation of plasmid DNA
Birnboim, H. C. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)00059-2 · Cited by 964
Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ
Kuzminov, A. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.4.751-813.1999 · Cited by 896
Self-citationCrystal Structures of Human Topoisomerase I in Covalent and Noncovalent Complexes with DNA
Redinbo, M. R., Stewart, L., Kuhn, P., Champoux, J. J., & Hol, W. G. J. (1998) · Science · doi:10.1126/science.279.5356.1504 · Cited by 889
Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death
Sonoda, E. (1998) · The EMBO Journal · doi:10.1093/emboj/17.2.598 · Cited by 791
Lim, D.-S., & Hasty, P. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.12.7133 · Cited by 696
Cellular and Molecular Biology
(2014) · Cellular and Molecular Biology · doi:10.14715/cmb/2013.60.1.4 · Cited by 580
Topoisomerase Poisons: Harnessing the Dark Side of Enzyme Mechanism
Froelich-Ammon, S. J., & Osheroff, N. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.37.21429 · Cited by 505
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Metadata
- Authors
- Kuzminov, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-07-17
- Volume
- 98
- Issue
- 15
- Pages
- 8241-8246
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2161340431
- Cited by
- 406
- References
- 34
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- green
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