Cited by 323Nanopore and Nanochannel Transport StudiesEpigenetics and DNA MethylationDNA and Nucleic Acid Chemistry

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Detection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA

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Laszlo, Andrew H., Derrington, Ian M., Brinkerhoff, Henry, Langford, Kyle W., Nova, Ian C., Samson, Jenny Mae, Bartlett, Joshua J., Pavlenok, Mikhail, Gundlach, Jens H.·2013·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1310240110PMC PMC3839702

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Abstract

Significance: Cells attach a methyl group (—CH 3 ) to certain cytosines in DNA to control gene expression. These methylation patterns change over time and can be related to cell differentiation and diseases such as cancer. Existing methylation detection techniques are not ideal for clinical use. We pulled single-stranded DNA molecules through the biological pore MspA and found that ion currents passing through the pore reveal the methylation sites with high confidence. Hydroxymethylation, which differs from methylation by only one oxygen atom, also produces distinct signals. This technique can be developed into a research tool and may ultimately lead to clinical tests.

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Authors: Laszlo, A. H., Derrington, I. M., Brinkerhoff, H., Langford, K. W., Nova, I. C., Samson, J. M., Bartlett, J. J., Pavlenok, M., & Gundlach, J. H.
Affiliations: University of Washington (US)
University of Alabama at Birmingham (US)
ORCID: Laszlo, Andrew H.
Brinkerhoff, Henry
Langford, Kyle W.
Nova, Ian C.
Gundlach, Jens H.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2013-10-28
Volume: 110
Issue: 47
Pages: 18904-18909
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1310240110
PMC: PMC3839702
OpenAlex: W2073105380
Cited by: 321
References: 38
Citations by year: 2026: 17 · 2025: 21 · 2024: 32 · 2023: 19 · 2022: 26 · 2021: 38 · 2020: 15 · 2019: 21 · 2018: 21 · 2017: 27 · 2016: 30 · 2015: 33 · 2014: 19 · 2013: 2
FWCI: 15.46
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Human Genome Research Institute (R01HG006321, R01HG005115)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Nanopore and Nanochannel Transport Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords: 5-methylcytosine, 5-hydroxymethylcytosine, DNA methylation, DNA hydroxymethylation, nanopore sequencing, single-molecule detection, epigenetic modifications, ionic currents, phi29 DNA polymerase, DNA strand passage
MeSH terms: Bayes Theorem, Cytosine, Models, Molecular, Molecular Structure, Porins, DNA Methylation, 5-Methylcytosine, Epigenomics, Nanopores

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Detection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA

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