The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading, and activation
Open accessAbstract
Significance: Platelets are cell fragments in the blood that initiate clot formation at the site of bleeding. Although the biological aspects of this process have been well characterized, whether platelets can detect and physiologically respond to the mechanical aspects of its local environment is unclear. Here, we show that platelets sense the stiffness of the underlying clot substrate, and increasing substrate stiffness increases platelet adhesion and spreading. Importantly, adhesion on stiffer substrates leads to higher levels of platelet activation. Mechanistically, we determined that Rac1, actin, and myosin activity mediate this process. This newfound capability of how platelets adjust their degree of activation based on the mechanical properties of their environment provides new insight into how clots are formed.
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Citations
Cites
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Activation of Platelet Function Through G Protein–Coupled Receptors
Offermanns, S. (2006) · Circulation Research · doi:10.1161/01.res.0000251742.71301.16 · Cited by 725
Integrins and Extracellular Matrix in Mechanotransduction
Schwartz, M. A. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005066 · Cited by 662
Cited by
A practical guide to hydrogels for cell culture
Caliari, S. R., & Burdick, J. A. (2016) · Nature Methods · doi:10.1038/nmeth.3839 · Cited by 2,075
Fibrin Formation, Structure and Properties
Weisel, J. W., & Litvinov, R. I. (2017) · Subcellular Biochemistry · doi:10.1007/978-3-319-49674-0_13 · Cited by 729
Janmey, P. A., Fletcher, D. A., & Reinhart-King, C. A. (2020) · Physiological Reviews · doi:10.1152/physrev.00013.2019 · Cited by 422
The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation
Moretti, L., Stalfort, J., Barker, T. H., & Abebayehu, D. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.101530 · Cited by 393
Self-citationBowman, S. L., Bi‐Karchin, J., Le, L., & Marks, M. S. (2019) · Traffic · doi:10.1111/tra.12646 · Cited by 219
Metadata
- Authors
- Qiu, Y., Brown, A. C., Myers, D. R., Sakurai, Y., Mannino, R. G., Tran, R., Ahn, B., Hardy, E. T., Kee, M. F., Kumar, S., Bao, G., Barker, T. H., & Lam, W. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2014-09-22
- Volume
- 111
- Issue
- 40
- Pages
- 14430-14435
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2097503680
- Cited by
- 213
- References
- 3
- FWCI
- 6.38
- Type
- journal article
- Language
- en
- Open access
- green
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- Qiu, Y., Brown, A. C., Myers, D. R., Sakurai, Y., Mannino, R. G., Tran, R., Ahn, B., Hardy, E. T., Kee, M. F., Kumar, S., Bao, G., Barker, T. H., & Lam, W. A. (2014). Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading, and activation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1322917111
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