A practical guide to hydrogels for cell culture
Open accessAbstract
There is growing appreciation of the role that the extracellular environment plays in regulating cell behavior. Mechanical, structural, and compositional cues, either alone or in concert, can drastically alter cell function. Biomaterials, and particularly hydrogels, have been developed and implemented to present defined subsets of these cues for investigating countless cellular processes as a means of understanding morphogenesis, aging, and disease. Although most scientists concede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recapitulating native cellular milieus, there is currently a knowledge barrier for many researchers in regard to the application of hydrogels for cell culture. Here, we introduce hydrogels to those who may be unfamiliar with procedures to culture and study cells with these systems, with a particular focus on commercially available hydrogels.
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Fibronectin-coated gelatin fibers contain RGD peptide binding domains that promote matrix-cell adhesion through integrins, unlike cellulose nanofibers and carbon nanotubes previously used in inks.
“In contrast to cellulose nanofiber and carbon nanotubes, previously used in inks 36–38, fibronectin-coated gelatin fibers contain arginine–glycine–aspartic (RGD) acid peptide binding domains that promote matrix-cell adhesion through integrins39.”
From Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles (2023) · cites this paper as [39]
The Alginate section (page unavailable) identifies RGD as an adhesive ligand that enables cell attachment, and “Selecting a hydrogel for cell culture” says some materials interact with cells through integrin–ligand interactions. The paper does not specifically say that fibronectin-coated gelatin fibers contain RGD domains or demonstrate adhesion through those fibers; the cellulose-nanofiber and carbon-nanotube comparison is cited separately. (LLM, conf=0.85)
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Self-citation
- Authors
- Caliari, S. R., & Burdick, J. A.
- Affiliations
- University of Pennsylvania (US)
- Corresponding author
- Burdick, Jason A
- Venue
- Nature Methods
- Published
- 2016-04-28
- Volume
- 13
- Issue
- 5
- Pages
- 405-414
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC5800304
- OpenAlex
- W2344920256
- Cited by
- 2,075
- References
- 98
- Citations by year
- 2026: 217 · 2025: 265 · 2024: 244 · 2023: 241 · 2022: 229 · 2021: 244 · 2020: 236 · 2019: 163 · 2018: 136 · 2017: 79 · 2016: 19
- FWCI
- 8.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (F32DK103463)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR056624)
- National Institute of Biomedical Imaging and Bioengineering (R01EB008722)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- hydrogels, cell culture, extracellular matrix mimics, mechanical cues, biomaterials, tissue culture plastic, cell behavior, morphogenesis, aging, disease models
- MeSH terms
- Polyacrylamides, Acrylic Resins, Alginates, Animals, Cell Adhesion, Cells, Cultured, Collagen, Extracellular Matrix, Fibrin, Glass, Hexuronic Acids, Humans, Polyethylene Glycols, Cell Culture Techniques, Hydrogels, Glucuronic Acid, Biomimetic Materials, Mice, Mesenchymal Stem Cells
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