Cited by 301Fungal and yeast genetics researchBiofuel production and bioconversionMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.

Engineering of yeast hexose transporters to transport d -xylose without inhibition by d -glucose

Open access

Farwick, Alexander, Bruder, Stefan, Schadeweg, Virginia, Oreb, Mislav, Boles, Eckhard·2014·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1323464111PMC PMC3986176

Open this paper in CoChat — free account

Abstract

All known D-xylose transporters are competitively inhibited by D-glucose, which is one of the major reasons hampering simultaneous fermentation of D-glucose and D-xylose, two primary sugars present in lignocellulosic biomass. We have set up a yeast growth-based screening system for mutant D-xylose transporters that are insensitive to the presence of D-glucose. All of the identified variants had a mutation at either a conserved asparagine residue in transmembrane helix 8 or a threonine residue in transmembrane helix 5. According to a homology model of the yeast hexose transporter Gal2 deduced from the crystal structure of the D-xylose transporter XylE from Escherichia coli, both residues are found in the same region of the protein and are positioned slightly to the extracellular side of the central sugar-binding pocket. Therefore, it is likely that alterations sterically prevent D-glucose but not D-xylose from entering the pocket. In contrast, changing amino acids that are supposed to directly interact with the C6 hydroxymethyl group of D-glucose negatively affected transport of both D-glucose and D-xylose. Determination of kinetic properties of the mutant transporters revealed that Gal2-N376F had the highest affinity for D-xylose, along with a moderate transport velocity, and had completely lost the ability to transport hexoses. These transporter versions should prove valuable for glucose-xylose cofermentation in lignocellulosic hydrolysates by Saccharomyces cerevisiae and other biotechnologically relevant organisms. Moreover, our data contribute to the mechanistic understanding of sugar transport because the decisive role of the conserved asparagine residue for determining sugar specificity has not been recognized before.

Integrity signals

Registry

Crossref · 10.1073/pnas.1323464111

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling
Arnold, K., Bordoli, L., Kopp, J., & Schwede, T. F. (2005) · Bioinformatics · doi:10.1093/bioinformatics/bti770 · Cited by 7,031
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
Boeke, J. D., Lacroute, F., & Fink, G. R. (1984) · Molecular and General Genetics MGG · doi:10.1007/bf00330984 · Cited by 2,354
A new efficient gene disruption cassette for repeated use in budding yeast
Güldener, U. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.13.2519 · Cited by 1,657
Hemicelluloses for fuel ethanol: A review
Gírio, F. M., Fonseca, C., Carvalheiro, F., Duarte, L. C., Marques, S., & Bogel‐Łukasik, R. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.088 · Cited by 1,648
Xylose fermentation by Saccharomyces cerevisiae
K�tter, P., & Ciriacy, M. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00167144 · Cited by 507
Crystal structure of a bacterial homologue of glucose transporters GLUT1–4
Sun, L., Zeng, X. W., Yan, C., Sun, X., Gong, X., Rao, Y., & Yan, N. (2012) · Nature · doi:10.1038/nature11524 · Cited by 483
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
Ha, S.-J., Galazka, J. M., Kim, S. R., Choi, J.-H., Yang, X., Seo, J., Glass, N. L., Cate, J. H. D., & Jin, Y. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1010456108 · Cited by 468
Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries
Hong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451
Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
Kuyper, M., HARTOG, M., Toirkens, M. J., Almering, M. J. H., Winkler, A. A., VANDIJKEN, J., & Pronk, J. T. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.09.010 · Cited by 404
High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
Kuyper, M., Harhangi, H. R., STAVE, A., Winkler, A. A., Jetten, M. S. M., DELAAT, W., DENRIDDER, J., den Camp, H. J. M. O., VANDIJKEN, J., & Pronk, J. T. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(03)00141-7 · Cited by 366

Show all 54 references

Research works printed but not linked: 1

Schrödinger (2010) The PyMOL Molecular Graphics System (Schrödinger Cambridge MA) Version 1.3r1.

Sorted by citations

Items 101–200 of 301

Novel transporters from Kluyveromyces marxianus and Pichia guilliermondii expressed in Saccharomyces cerevisiae enable growth on l ‐arabinose and d ‐xylose
Knoshaug, E. P., Vidgren, V., Magalhães, F., Jarvis, E. E., Franden, M. A., Zhang, M., & Singh, A. (2015) · Yeast · doi:10.1002/yea.3084 · Cited by 45
Functional Analysis of Two l -Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae
Li, J., Xu, J., Cai, P., Wang, B., Ma, Y., Benz, J. P., & Tian, C. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.00165-15 · Cited by 45
Assessing the effect of d-xylose on the sugar signaling pathways of Saccharomyces cerevisiae in strains engineered for xylose transport and assimilation
Osiro, K. O., Brink, D. P., Borgström, C., Wasserstrom, L., Carlquist, M., & Gorwa‐Grauslund, M. F. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox096 · Cited by 45
An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae
Henritzi, S., Fischer, M., Grininger, M., Oreb, M., & Boles, E. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1149-1 · Cited by 45
Self-citation
Utilization of xylose by engineered strains of Ashbya gossypii for the production of microbial oils
Díaz-Fernández, D., Lozano‐Martínez, P., Buey, R. M., Revuelta, J. L., & Jiménez, A. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0685-9 · Cited by 44
Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains
Varela, J. A., Montini, N., Scully, D., der Ploeg, R. V., Oreb, M., Boles, E., Hirota, J., Akada, R., Hoshida, H., & Morrissey, J. P. (2017) · FEMS Yeast Research · doi:10.1093/femspd/fox021 · Cited by 44
Self-citation
Pentose Metabolism in Saccharomyces cerevisiae : The Need to Engineer Global Regulatory Systems
Gopinarayanan, V. E., & Nair, N. U. (2018) · Biotechnology Journal · doi:10.1002/biot.201800364 · Cited by 44
D-Xylose Sensing in Saccharomyces cerevisiae: Insights from D-Glucose Signaling and Native D-Xylose Utilizers
Brink, D. P., Borgström, C., Persson, V. C., Osiro, K. O., & Gorwa‐Grauslund, M. F. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222212410 · Cited by 44
Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications
Soares‐Silva, I., Ribas, D. M. N., Sousa-Silva, M., Azevedo‐Silva, J., Rendulić, T., & Casal, M. (2020) · FEMS Microbiology Letters · doi:10.1093/femsle/fnaa118 · Cited by 45
The amino‐terminal tail of Hxt11 confers membrane stability to the Hxt2 sugar transporter and improves xylose fermentation in the presence of acetic acid
Shin, H. Y., Nijland, J. G., de Waal, P. P., & Driessen, A. J. M. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26322 · Cited by 42
Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
Vasylyshyn, R. V., Kurylenko, O. O., Ruchała, J., Shevchuk, N., Kuliešienė, N., Khroustalyova, G., Rapoport, A. I., Daugelavičius, R., Dmytruk, K. V., & Sibirny, A. А. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01354-9 · Cited by 42
Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously
Kuanyshev, N., Deewan, A., Jagtap, S. S., Liu, J., Selvam, B., Chen, L., Shukla, D., Rao, C. V., & Jin, Y. (2021) · Biotechnology Journal · doi:10.1002/biot.202100238 · Cited by 41
Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars
Fan, L., Zhang, Z., Mei, S., Lu, Y., Mei, L., Wang, Z.-Y., Yang, J., Yang, S., & Tan, T. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0554-6 · Cited by 40
Metabolic Engineering of Saccharomyces cerevisiae for Enhanced Carotenoid Production From Xylose-Glucose Mixtures
Su, B., Song, D., & Zhu, H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00435 · Cited by 39
Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinant Saccharomyces cerevisiae strains at high xylose concentrations
Bueno, J. G. R., Borelli, G., Côrrea, T. L. R., Fiamenghi, M. B., José, J., Carvalho, M. D. O., de Oliveira, L. C., Pereira, G. A. G., & dos Santos, L. V. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01782-0 · Cited by 39
Engineering of xylose metabolism in Escherichia coli for the production of valuable compounds
Bañares, A. B., Nisola, G. M., Valdehuesa, K. N. G., Lee, W., & Chung, W. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1873243 · Cited by 39
Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium
Shin, J., Liao, S., Kuanyshev, N., Xin, Y., Kim, C.-W., Lu, T., & Jin, Y. (2024) · Nature Communications · doi:10.1038/s41467-024-45011-w · Cited by 38
Improved xylose uptake inSaccharomyces cerevisiaedue to directed evolution of galactose permease Gal2 for sugar co-consumption
Reznicek, O., Facey, S. J., de Waal, P. P., Teunissen, A. W. R. H., de Bont, J. A. M., Nijland, J. G., Driessen, A. J. M., & Hauer, B. (2015) · Journal of Applied Microbiology · doi:10.1111/jam.12825 · Cited by 38
Biorefinery Platform for Spathaspora passalidarum NRRL Y-27907 in the Production of Ethanol, Xylitol, and Single Cell Protein from Sugarcane Bagasse
Bonan, C. I. D. G., Tramontina, R., dos Santos, M. W., Biazi, L. E., Soares, L. B., Pereira, I. D. O., Hoffmam, Z. B., Coutouné, N., Squina, F. M., Robl, D., et al. (2021) · BioEnergy Research · doi:10.1007/s12155-021-10255-7 · Cited by 38
Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila
Li, J., Chen, B., Gu, S., Zhao, Z., Liu, Q., Sun, T., Zhang, Y., Wu, T., Liu, D., Sun, W., et al. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-021-02042-5 · Cited by 38
Synthetic Biology of Yeast
Liu, Z., Zhang, Y., & Nielsen, J. (2019) · Biochemistry · doi:10.1021/acs.biochem.8b01236 · Cited by 40
The genome sequence of the popular hexose-transport-deficient Saccharomyces cerevisiae strain EBY.VW4000 reveals LoxP/Cre-induced translocations and gene loss
Solis-Escalante, D., van den Broek, M. A., Kuijpers, N. G. A., Pronk, J. T., Boles, E., Daran, J.-M. G., & Daran-Lapujade, P. A. S. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fou004 · Cited by 37
Self-citation
Natural Variation in the Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast
Higgins, D. A., Young, M. K. M., Tremaine, M. T., Sardi, M. I., Fletcher, J. M., Agnew, M. D., Liu, L., Dickinson, Q., Peris, D., Wrobel, R. L., et al. (2018) · Genetics · doi:10.1534/genetics.118.301161 · Cited by 36
Enhanced production of xylitol from xylose by expression of Bacillus subtilis arabinose:H + symporter and Scheffersomyces stipitis xylose reductase in recombinant Saccharomyces cerevisiae
Kim, H., Lee, H.-S., Park, H., Lee, D., Boles, E., Chung, D., & Park, Y. (2017) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2017.07.014 · Cited by 34
Self-citation
Fuel Ethanol Production From Lignocellulosic Biomass
Bai, F., Yang, S., & Ho, N. W. Y. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64046-8.00150-6 · Cited by 34
Harnessing Nature's Anaerobes for Biotechnology and Bioprocessing
Podolsky, I. A., Seppälä, S., Lankiewicz, T. S., Brown, J. L., Swift, C. L., & O’Malley, M. (2019) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-060718-030340 · Cited by 37
Evolution and functional diversification of yeast sugar transporters
Donzella, L., Sousa, M. J., & Morrissey, J. P. (2023) · Essays in Biochemistry · doi:10.1042/ebc20220233 · Cited by 32
Identification of Important Amino Acids in Gal2p for Improving the L-arabinose Transport and Metabolism in Saccharomyces cerevisiae
Wang, C., Li, Y., Qiu, C., Wang, S., Ma, J., Shen, Y., Zhang, Q., Du, B., Ding, Y., & Bao, X. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01391 · Cited by 32
Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production
Gao, M., Zhao, Y., Yao, Z., Su, Q., Beek, P. V., & Shao, Z. (2023) · Nature Communications · doi:10.1038/s41467-023-43049-w · Cited by 30
Directed Evolution of Membrane Transport Using Synthetic Selections
Bali, A. P., Genee, H. J., & Sommer, M. O. A. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00407 · Cited by 30
Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari
Guamán, L. P., Barba‐Ostria, C., Zhang, F., Oliveira-Filho, E. R., Gomez, J. G. C., & da Silva, L. F. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0924-9 · Cited by 30
Lipid production via simultaneous conversion of glucose and xylose by a novel yeast, Cystobasidium iriomotense
Tanimura, A., Sugita, T., Endoh, R., Ohkuma, M., Kishino, S., Ogawa, J., Shima, J., & Takashima, M. (2018) · PLoS ONE · doi:10.1371/journal.pone.0202164 · Cited by 29
In-Depth Two-Stage Transcriptional Reprogramming and Evolutionary Engineering of Saccharomyces cerevisiae for Efficient Bioethanol Production from Xylose with Acetate
Zhang, C., Xue, Q., Hou, J., Mohsin, A., Zhang, M., Guo, M., Zhu, Y., Bao, J., Wang, J., Xiao, W., et al. (2019) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.9b05095 · Cited by 29
Increased xylose affinity of Hxt2 through gene shuffling of hexose transporters in Saccharomyces cerevisiae
Nijland, J. G., Shin, H. Y., de Waal, P. P., Klaassen, P., & Driessen, A. J. M. (2017) · Journal of Applied Microbiology · doi:10.1111/jam.13670 · Cited by 28
Determinants of sugar-induced influx in the mammalian fructose transporter GLUT5
McComas, S. E., Reichenbach, T., Mitrovic, D., Alleva, C., Bonaccorsi, M., Delemotte, L., & Drew, D. (2023) · eLife · doi:10.7554/elife.84808 · Cited by 27
Characterization of two sugar transporters responsible for efficient xylose uptake in an oleaginous yeast Candida tropicalis SY005
Chattopadhyay, A., Singh, R., Das, A. K., & Maiti, M. K. (2020) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2020.108645 · Cited by 27
A C6/C5 co‐fermentingSaccharomyces cerevisiaestrain with the alleviation of antagonism between xylose utilization and robustness
Wei, F., Li, M., Wang, M., Li, H., Li, Z., Qin, W., Wei, T., Zhao, J., & Bao, X. (2020) · GCB Bioenergy · doi:10.1111/gcbb.12778 · Cited by 27
Heterologous expression of xylose specific transporter improves xylose utilization by recombinant Zymomonas mobilis strain in presence of glucose
Sarkar, P., Goswami, G., Mukherjee, M., & Das, D. (2021) · Process Biochemistry · doi:10.1016/j.procbio.2021.01.006 · Cited by 27
Converging conversion – using promiscuous biocatalysts for the cell-free synthesis of chemicals from heterogeneous biomass
Sutiono, S., Pick, A., & Sieber, V. (2021) · Green Chemistry · doi:10.1039/d0gc04288a · Cited by 27
Identification of novel pentose transporters in Kluyveromyces marxianus using a new screening platform
Donzella, L., Varela, J. A., Sousa, M. J., & Morrissey, J. P. (2021) · FEMS Yeast Research · doi:10.1093/femsyr/foab026 · Cited by 26
Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
Sun, L., Wu, B., Zhang, Z., Yan, J., Liu, P., Song, C., Shabbir, S., Zhu, Q., Yang, S., Peng, N., et al. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-021-02069-8 · Cited by 26
TranCEP: Predicting the substrate class of transmembrane transport proteins using compositional, evolutionary, and positional information
Alballa, M., Aplop, F., & Butler, G. (2020) · PLoS ONE · doi:10.1371/journal.pone.0227683 · Cited by 26
Mapping the membrane proteome of anaerobic gut fungi identifies a wealth of carbohydrate binding proteins and transporters
Seppälä, S., Solomon, K., Gilmore, S. P., Henske, J. K., & O’Malley, M. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0611-7 · Cited by 29
Regulation of Transgene Expression by the Natural Sweetener Xylose
Galvan, S., Madderson, O., Xue, S., Teixeira, A. P., & Fussenegger, M. (2022) · Advanced Science · doi:10.1002/advs.202203193 · Cited by 26
Re-routing the hemicellulosic fraction of lignocellulosic biomass toward value added products: A pragmatic bio refinery approach
Chandukishore, T., Das, S., Das, P. K., Veeranki, V. D., & Prabhu, A. A. (2024) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2024.111971 · Cited by 25
Combined Cell Surface Display of β‐ d ‐Glucosidase (BGL), Maltose Transporter (MAL11), and Overexpression of Cytosolic Xylose Reductase (XR) in Saccharomyces cerevisiae Enhance Cellobiose/Xylose Coutilization for Xylitol Bioproduction from Lignocellulosic Biomass
Guirimand, G. G. Y., Bamba, T., Matsuda, M., Inokuma, K., Morita, K., Kitada, Y., Kobayashi, Y., Yukawa, T., Sasaki, K., Ogino, C., et al. (2019) · Biotechnology Journal · doi:10.1002/biot.201800704 · Cited by 25
Recent progress in engineering yeast producers of cellulosic ethanol
Vasylyshyn, R. V., Ruchała, J., Dmytruk, K. V., & Sibirny, A. А. (2025) · FEMS Yeast Research · doi:10.1093/femsyr/foaf035 · Cited by 24
Lignocellulosic xylitol production from corncob using engineered Kluyveromycesmarxianus
Zhang, J., Xu, T., Wang, X., Jing, X., Zhang, J., Hong, J., Xu, J., & Wang, J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1029203 · Cited by 24
Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake
Verhoeven, M. D., Bracher, J. M., Nijland, J. G., Bouwknegt, J., Daran, J.-M. G., Driessen, A. J. M., van Maris, A. J. A., & Pronk, J. T. (2018) · FEMS Yeast Research · doi:10.1093/femsyr/foy062 · Cited by 24
Experimental evolution: its principles and applications in developing stress-tolerant yeasts
Swamy, K. B. S., & Zhou, N. (2019) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-09616-2 · Cited by 25
Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola
Claus, S., Jezierska, S., Elbourne, L. D. H., & Bogaert, I. V. (2022) · BMC Genomics · doi:10.1186/s12864-021-08177-x · Cited by 24
Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast
Fiamenghi, M. B., Bueno, J. G. R., Camargo, A. P., Borelli, G., Carazzolle, M. F., Pereira, G. A. G., dos Santos, L. V., & José, J. (2022) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-022-02153-7 · Cited by 23
Identification of an important motif that controls the activity and specificity of sugar transporters
Wang, M., Yu, C., & Zhao, H. (2016) · Biotechnology and Bioengineering · doi:10.1002/bit.25926 · Cited by 23
Fueling the future with biomass: Processes and pathways for a sustainable supply of hydrocarbon fuels and biogas
Junne, S. G., & Kabisch, J. (2016) · Engineering in Life Sciences · doi:10.1002/elsc.201600112 · Cited by 24
De novo production of aromatic m-cresol in Saccharomyces cerevisiae mediated by heterologous polyketide synthases combined with a 6-methylsalicylic acid decarboxylase
Hitschler, J., & Boles, E. (2019) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00093 · Cited by 23
Self-citation
Secretion of 2,3-dihydroxyisovalerate as a limiting factor for isobutanol production in Saccharomyces cerevisiae
Generoso, W. C., Brinek, M., Dietz, H., Oreb, M., & Boles, E. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox029 · Cited by 22
Self-citation
A Growth-Based Screening System for Hexose Transporters in Yeast
Boles, E., & Oreb, M. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7507-5_10 · Cited by 22
Self-citation
Influence of glucose on xylose metabolization by Spathaspora passalidarum
Ribeiro, L. E., Albuini, F. M., de Castro, A. G., Campos, V. J., de Souza, G. B., Mendonça, J. G. P., Rosa, C. A., Mendes, T. A. D. O., Santana, M. F., da Silveira, W. B., et al. (2021) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2021.103624 · Cited by 22
Fermentation performance of a Mexican native Clavispora lusitaniae strain for xylitol and ethanol production from xylose, glucose and cellobiose
Ochoa-Chacón, A., Ramos-Valdivia, A. C., Poggi-Varaldo, H. M., Villa‐Tanaca, L., Martı́nez, A., & Ponce‐Noyola, T. (2022) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2022.110094 · Cited by 21
Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts
Coradetti, S. T., Adamczyk, P., Liu, D., Gao, Y., Otoupal, P. B., Geiselman, G. M., Webb‐Robertson, B., Burnet, M., Kim, Y., Burnum-Johnson, K., et al. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02148-5 · Cited by 21
Optimisation of trans-cinnamic acid and hydrocinnamyl alcohol production with recombinant Saccharomyces cerevisiae and identification of cinnamyl methyl ketone as a by-product
Gottardi, M., Grün, P., Bode, H. B., Hoffmann, T., Schwab, W. G., Oreb, M., & Boles, E. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox091 · Cited by 21
Self-citation
Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity
Rojas, S., Schadeweg, V., Kirchner, F., Boles, E., & Oreb, M. (2021) · Scientific Reports · doi:10.1038/s41598-021-03822-7 · Cited by 21
Self-citation
Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei
Havukainen, S., Pujol‐Giménez, J., Valkonen, M. J., Hediger, M. A., & Landowski, C. P. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01666-4 · Cited by 20
Key amino acid residues of the AGT1 permease required for maltotriose consumption and fermentation by Saccharomyces cerevisiae
Trichez, D., Knychala, M. M., de Figueiredo, C. M., Júnior, S. L. A., Silva, M. A., Miletti, L. C., Araujo, P. S. D., & Stambuk, B. U. (2018) · Journal of Applied Microbiology · doi:10.1111/jam.14161 · Cited by 20
Evolutionary Engineering Improved d -Glucose/Xylose Cofermentation of Yarrowia lipolytica
Zhou, L., Wen, Z., Wang, Z., Zhang, Y., Ledesma‐Amaro, R., & Jin, M. (2020) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.0c00896 · Cited by 20
Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation
Taveira, I. C., Carraro, C. B., Nogueira, K. M. V., Pereira, L. M. S., Bueno, J. G. R., Fiamenghi, M. B., dos Santos, L. V., & Silva, R. N. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1452240 · Cited by 19
Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering
Geng, B., Jia, X., Peng, X., & Han, Y. (2022) · Metabolic Engineering Communications · doi:10.1016/j.mec.2022.e00211 · Cited by 19
Reducing Carbon Intensity of Food and Fuel Production Whilst Lowering Land-Use Impacts of Biofuels
Attfield, P. V., Bell, P., & Grobler, A. S. (2023) · Fermentation · doi:10.3390/fermentation9070633 · Cited by 19
Heterologous transporters from anaerobic fungi bolster fluoride tolerance in Saccharomyces cerevisiae
Seppälä, S., Yoo, J. I., Yur, D., & O’Malley, M. (2019) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00091 · Cited by 20
Xylose and yeasts: A story beyond xylitol production
Estrada-Ávila, A. K., González‐Hernández, J. C., Calahorra, M., Sánchez, N. S., & Peña, A. (2022) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2022.130154 · Cited by 19
Increasing carbon source uptake rates to improve chemical productivity in metabolic engineering
Yan, Q., & Fong, S. S. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.06.001 · Cited by 19
Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production
Pereira, L. M. S., Taveira, I. C., Maués, D. B., de Paula, R. G., & Silva, R. N. (2025) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-025-13408-2 · Cited by 18
Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae
Zhang, C., Acosta‐Sampson, L., Yu, V. Y., & Cate, J. H. D. (2017) · PLoS ONE · doi:10.1371/journal.pone.0184730 · Cited by 17
Directing enzyme devolution for biosynthesis of alkanols and 1,n-alkanediols from natural polyhydroxy compounds
Dai, L., Tao, F., Tang, H., Guo, Y., Shen, Y., & Xu, P. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.09.005 · Cited by 17
Bio-Products from Sugar-Based Fermentation Processes
Kádár, Z., & Fonseca, C. (2019) · Springer eBooks · doi:10.1007/978-3-030-10961-5_12 · Cited by 17
Characterization and engineering of the xylose-inducible xylP promoter for use in mold fungal species
Yap, A., Glarcher, I., Misslinger, M., & Haas, H. (2022) · Metabolic Engineering Communications · doi:10.1016/j.mec.2022.e00214 · Cited by 17
Efficient, D-glucose insensitive, growth on D-xylose by an evolutionary engineered Saccharomyces cerevisiae strain
Nijland, J. G., Li, X., Shin, H. Y., de Waal, P. P., & Driessen, A. J. M. (2019) · FEMS Yeast Research · doi:10.1093/femsyr/foz083 · Cited by 17
Engineering transport systems for microbial production
Onyeabor, M., Martinez, R., Kurgan, G., & Wang, X. (2020) · Advances in applied microbiology · doi:10.1016/bs.aambs.2020.01.002 · Cited by 17
De novo biosynthesis of 8-hydroxyoctanoic acid via a medium-chain length specific fatty acid synthase and cytochrome P450 in Saccharomyces cerevisiae
Wernig, F., Boles, E., & Oreb, M. (2019) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00111 · Cited by 16
Self-citation
Assessment of the ability of Meyerozyma guilliermondii P14 to produce second-generation bioethanol from giant reed (Arundo donax) biomass
Sidana, A., Kaur, S., & Yadav, S. K. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-02211-4 · Cited by 16
Sugar transport for enhanced xylose utilization in Ashbya gossypii
Díaz-Fernández, D., Muñoz‐Fernández, G., Martín, V. I., Revuelta, J. L., & Jiménez, A. (2020) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-020-02320-5 · Cited by 15
Fermentation of D-xylose to Ethanol by Saccharomyces cerevisiae CAT-1 Recombinant Strains
Coimbra, L., Malán, A. K., Fagúndez, A., Guigou, M. D., Lareo, C., Fernández, B., Pratto, M., & Batista, S. B. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10514-1 · Cited by 15
Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose
Vasylyshyn, R. V., Dmytruk, O. V., Sibirny, A. А., & Ruchała, J. (2024) · FEMS Yeast Research · doi:10.1093/femsyr/foae007 · Cited by 15
Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
Zhang, W., Zhang, J., Zhao, X., Zhang, Z., He, S., Bian, X., Wang, H., Zhang, C., & Lü, W. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.153120 · Cited by 15
Directed evolution of Zymomonas mobilis sugar facilitator Glf to overcome glucose inhibition
Kurgan, G., Onyeabor, M., Holland, S. C., Taylor, E., Schneider, A., Kurgan, L., Billings, T., & Wang, X. (2021) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuab066 · Cited by 15
d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae
Nijland, J. G., Zhang, X., & Driessen, A. J. M. (2023) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-023-02320-4 · Cited by 15
Yeast as a tool to express sugar acid transporters with biotechnological interest
Ribas, D. M. N., Sá‐Pessoa, J., Soares‐Silva, I., Paiva, S., Nygård, Y., Ruohonen, L., Penttilä, M. E., & Casal, M. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox005 · Cited by 15
Nicotinic acid availability impacts redox cofactor metabolism in Saccharomyces cerevisiae during alcoholic fermentation
Duncan, J. D., Setati, M. E., & Divol, B. (2024) · FEMS Yeast Research · doi:10.1093/femsyr/foae015 · Cited by 14
Genetic Improvement of Conventional and Nonconventional Yeasts for the Production of First- and Second-Generation Ethanol
Dmytruk, K. V., Kurylenko, O. O., Ruchała, J., Abbas, C. A., & Sibirny, A. А. (2017) · Springer eBooks · doi:10.1007/978-3-319-58829-2_1 · Cited by 14
Engineering xylose fermentation in an industrial yeast: continuous cultivation as a tool for selecting improved strains
Basso, T. P., Procópio, D. P., Petrin, T. H. C., Giacon, T. G., Jin, Y., Basso, T. O., & Basso, L. C. (2023) · Letters in Applied Microbiology · doi:10.1093/lambio/ovad077 · Cited by 13
Transporter engineering promotes the co-utilization of glucose and xylose by Candida glycerinogenes for d-xylonate production
Qiao, Y., Li, C., Lu, X., Zong, H., & Zhuge, B. (2021) · Biochemical Engineering Journal · doi:10.1016/j.bej.2021.108150 · Cited by 15
Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic RegionsPreprint
Ellis, H. J., Chan, M. C., Selvam, B., Walter, E., Devlin, C. A., Szymanski, S. K., Henry, L. K., Shukla, D., & Procko, E. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.04.19.440442 · Cited by 16
2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity
Laussel, C., Albanèse, V., García-Rodríguez, F. J., Ballin, A., Defenouillère, Q., & Sébastien Léon, A. (2022) · PLoS Genetics · doi:10.1371/journal.pgen.1010169 · Cited by 13
Formation and resolution of meiotic chromosome entanglements and interlocks
Olaya, I., Burgess, S. M., & Rog, O. (2024) · Journal of Cell Science · doi:10.1242/jcs.262004 · Cited by 12
Microaerobic conversion of xylose to ethanol in recombinant Saccharomyces cerevisiae SX6 MUT expressing cofactor-balanced xylose metabolic enzymes and deficient in ALD6
Jo, S.-E., Seong, Y., Lee, H.-S., Lee, S. M., Kim, S.-J., Park, K., & Park, Y. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.04.005 · Cited by 12
Enhanced Production of Bioethanol by Fermentation of Autohydrolyzed and C4mimOAc-Treated Sugarcane Bagasse Employing Various Yeast Strains
Hashmi, M., Shah, A. A., Hameed, A., & Ragauskas, A. J. (2017) · Energies · doi:10.3390/en10081207 · Cited by 12
l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
Oehling, V., Klaassen, P., Frick, O., Dusny, C., & Schmid, A. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1231-8 · Cited by 12
Sequential Production of ᴅ-xylonate and Ethanol from Non-Detoxified Corncob at Low-pH by Pichia kudriavzevii via a Two-Stage Fermentation Strategy
Ji, H., Xu, K., Dong, X., Sun, D., & Jin, L. (2021) · Journal of Fungi · doi:10.3390/jof7121038 · Cited by 12
Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition
Queiroz, S. D. S., Oliva, B., Silva, T. D. F., Segato, F., & Felipe, M. G. A. (2022) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-022-12005-x · Cited by 12
Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts
Trichez, D., Steindorff, A. S., Morais, W. G., Vilela, N., Bergmann, J. C., Formighieri, E. F., Gonçalves, S. B., & de Almeida, J. R. M. (2023) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12362-1 · Cited by 12

Authors: Farwick, A., Bruder, S., Schadeweg, V., Oreb, M., & Boles, E.
Affiliations: Goethe University Frankfurt (DE)
ORCID: Farwick, Alexander
Bruder, Stefan
Oreb, Mislav
Boles, Eckhard
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2014-03-24
Volume: 111
Issue: 14
Pages: 5159-5164
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1323464111
PMC: PMC3986176
OpenAlex: W2118069954
Cited by: 298
References: 54
Citations by year: 2026: 20 · 2025: 12 · 2024: 17 · 2023: 20 · 2022: 25 · 2021: 35 · 2020: 28 · 2019: 26 · 2018: 26 · 2017: 35 · 2016: 26 · 2015: 22 · 2014: 6
FWCI: 12.16
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: hexose transporters, GAL2, XylE, sugar specificity, competitive inhibition, lignocellulosic biomass, Saccharomyces cerevisiae
MeSH terms: Biological Transport, Glucose, Hydrolysis, Models, Molecular, Monosaccharide Transport Proteins, Saccharomyces cerevisiae, Xylose, Protein Engineering, Mutagenesis, Site-Directed

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Engineering of yeast hexose transporters to transport d -xylose without inhibition by d -glucose

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks