Cited by 301Fungal and yeast genetics researchBiofuel production and bioconversionMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.

Engineering of yeast hexose transporters to transport d -xylose without inhibition by d -glucose

Open access

Farwick, Alexander, Bruder, Stefan, Schadeweg, Virginia, Oreb, Mislav, Boles, Eckhard·2014·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1323464111PMC PMC3986176

Open this paper in CoChat — free account

Abstract

All known D-xylose transporters are competitively inhibited by D-glucose, which is one of the major reasons hampering simultaneous fermentation of D-glucose and D-xylose, two primary sugars present in lignocellulosic biomass. We have set up a yeast growth-based screening system for mutant D-xylose transporters that are insensitive to the presence of D-glucose. All of the identified variants had a mutation at either a conserved asparagine residue in transmembrane helix 8 or a threonine residue in transmembrane helix 5. According to a homology model of the yeast hexose transporter Gal2 deduced from the crystal structure of the D-xylose transporter XylE from Escherichia coli, both residues are found in the same region of the protein and are positioned slightly to the extracellular side of the central sugar-binding pocket. Therefore, it is likely that alterations sterically prevent D-glucose but not D-xylose from entering the pocket. In contrast, changing amino acids that are supposed to directly interact with the C6 hydroxymethyl group of D-glucose negatively affected transport of both D-glucose and D-xylose. Determination of kinetic properties of the mutant transporters revealed that Gal2-N376F had the highest affinity for D-xylose, along with a moderate transport velocity, and had completely lost the ability to transport hexoses. These transporter versions should prove valuable for glucose-xylose cofermentation in lignocellulosic hydrolysates by Saccharomyces cerevisiae and other biotechnologically relevant organisms. Moreover, our data contribute to the mechanistic understanding of sugar transport because the decisive role of the conserved asparagine residue for determining sugar specificity has not been recognized before.

Integrity signals

Registry

Crossref · 10.1073/pnas.1323464111

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling
Arnold, K., Bordoli, L., Kopp, J., & Schwede, T. F. (2005) · Bioinformatics · doi:10.1093/bioinformatics/bti770 · Cited by 7,031
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
Boeke, J. D., Lacroute, F., & Fink, G. R. (1984) · Molecular and General Genetics MGG · doi:10.1007/bf00330984 · Cited by 2,354
A new efficient gene disruption cassette for repeated use in budding yeast
Güldener, U. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.13.2519 · Cited by 1,657
Hemicelluloses for fuel ethanol: A review
Gírio, F. M., Fonseca, C., Carvalheiro, F., Duarte, L. C., Marques, S., & Bogel‐Łukasik, R. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.088 · Cited by 1,648
Xylose fermentation by Saccharomyces cerevisiae
K�tter, P., & Ciriacy, M. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00167144 · Cited by 507
Crystal structure of a bacterial homologue of glucose transporters GLUT1–4
Sun, L., Zeng, X. W., Yan, C., Sun, X., Gong, X., Rao, Y., & Yan, N. (2012) · Nature · doi:10.1038/nature11524 · Cited by 483
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
Ha, S.-J., Galazka, J. M., Kim, S. R., Choi, J.-H., Yang, X., Seo, J., Glass, N. L., Cate, J. H. D., & Jin, Y. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1010456108 · Cited by 468
Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries
Hong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451
Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
Kuyper, M., HARTOG, M., Toirkens, M. J., Almering, M. J. H., Winkler, A. A., VANDIJKEN, J., & Pronk, J. T. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.09.010 · Cited by 404
High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by ?
Kuyper, M., Harhangi, H. R., STAVE, A., Winkler, A. A., Jetten, M. S. M., DELAAT, W., DENRIDDER, J., den Camp, H. J. M. O., VANDIJKEN, J., & Pronk, J. T. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(03)00141-7 · Cited by 366

Show all 54 references

Research works printed but not linked: 1

Schrödinger (2010) The PyMOL Molecular Graphics System (Schrödinger Cambridge MA) Version 1.3r1.

Sorted by citations

Items 201–300 of 301

Glucose-induced internalization of the S. cerevisiae galactose permease Gal2 is dependent on phosphorylation and ubiquitination of its aminoterminal cytoplasmic tail
Rojas, S., Schmidl, S., Boles, E., & Oreb, M. (2021) · FEMS Yeast Research · doi:10.1093/femsyr/foab019 · Cited by 12
Self-citation
Identification of key residues for efficient glucose transport by the hexose transporter CgHxt4 in high sugar fermentation yeast Candida glycerinogenes
Qiao, Y., Li, C., Lu, X., Zong, H., & Zhuge, B. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-021-11567-6 · Cited by 11
A previously unidentified sugar transporter for engineering of high-yield Streptomyces
Dong, Z., Li, L., Du, G., Zhang, Y., Wang, X., Li, S., & Xiang, W. (2024) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12964-9 · Cited by 11
The Renaissance of Neurospora crassa: How a Classical Model System is Used for Applied Research
Seibert, T., Thieme, N., & Benz, J. P. (2016) · Fungal biology · doi:10.1007/978-3-319-27951-0_3 · Cited by 11
Construction of artificial membrane transport metabolons – an emerging strategy in metabolic engineering
Oreb, M. (2020) · FEMS Microbiology Letters · doi:10.1093/femsle/fnaa027 · Cited by 11
Self-citation
An overview of xylose valorization through its conversion into high-value chemicals by yeast
Queiroz, S. D. S., Felipe, M. D. G. D. A., & Mussatto, S. I. (2024) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2024.107428 · Cited by 10
Process optimization for saccharification and fermentation of the Organic Fraction of Municipal Solid Waste (OFMSW) to maximize ethanol production performance
Varghese, S., Demeke, M. M., Verhé, R., Redant, E., Cruyssen, C. V., & Thevelein, J. M. (2023) · Bioresource Technology Reports · doi:10.1016/j.biteb.2023.101681 · Cited by 10
An atlas of rational genetic engineering strategies for improved xylose metabolism in Saccharomyces cerevisiae
Vargas, B. D. O., dos Santos, J. R., Pereira, G. A. G., & de Mello, F. D. S. B. (2023) · PeerJ · doi:10.7717/peerj.16340 · Cited by 10
Online monitoring of the redox potential in microaerobic and anaerobic Scheffersomyces stipitis fermentations
Bonan, C. I. D. G., Biazi, L. E., Santos, S. C., Soares, L. B., Dionísio, S. R., Hoffmam, Z. B., da Costa, A. C., & Ienczak, J. L. (2019) · Biotechnology Letters · doi:10.1007/s10529-019-02674-6 · Cited by 10
Datamining and functional environmental genomics reassess the phylogenetics and functional diversity of fungal monosaccharide transporters
Barbi, F., Vallon, L., Guerrero‐Galán, C., Zimmermann, S., Melayah, D., Abrouk, D., Doré, J., Lemaire, M., Fraissinet‐Tachet, L., Luis, P., et al. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-11076-y · Cited by 10
Development of the Thermotolerant Methylotrophic Yeast Hansenula polymorpha as Efficient Ethanol Producer
Dmytruk, K. V., Kurylenko, O. O., Ruchała, J., Ishchuk, O. P., & Sibirny, A. А. (2017) · Springer eBooks · doi:10.1007/978-981-10-2621-8_11 · Cited by 9
Improving the productivity of Candida glycerinogenes in the fermentation of ethanol from non‐detoxified sugarcane bagasse hydrolysate by a hexose transporter mutant
Qiao, Y., Zhou, J., Lu, X., Zong, H., & Zhuge, B. (2021) · Journal of Applied Microbiology · doi:10.1111/jam.15059 · Cited by 9
Recent technologies in second-generation bioethanol production from biomass: A review
Abd, A. N., Shakor, Z. M., Al-Zuhairi, F. K., & Al–Sheikh, F. (2024) · International Journal of Green Energy · doi:10.1080/15435075.2024.2432572 · Cited by 8
Xylose-Driven Metabolic Reprogramming in Saccharomyces cerevisiae for Enhancing p-Coumaric Acid Production
Zhao, Y., Xiao, Z., Wang, Y., Tan, X., Zhang, S., Lu, Q., Hu, F., Zuo, S., Shan, Y., Liu, J., et al. (2025) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00792 · Cited by 8
Development of Synthetic Microbial Platforms to Convert Lignocellulosic Biomass to Biofuels
(2017) · Advances in bioenergy · doi:10.1016/bs.aibe.2016.12.001 · Cited by 8
Tiny bugs play big role
Yi, Y. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-818862-0.00007-8 · Cited by 8
Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase
Du, C., Li, Y., He, Y., Su, L., Wang, H., Yuan, W., & Bai, F. (2022) · Green Chemistry · doi:10.1039/d2gc02311c · Cited by 8
Characterisation of a catalytic triad and reaction selectivity in the dual mechanism of the catalyse hydride transfer in xylitol phosphate dehydrogenase
Razali, S. A., & Shamsir, M. S. (2020) · Journal of Molecular Graphics and Modelling · doi:10.1016/j.jmgm.2020.107548 · Cited by 8
The novel properties of Kluyveromyces marxianus glucose sensor/receptor repressor pathway and the construction of glucose repression-released strains
Wang, L., Wang, A., Wang, D., & Hong, J. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02138-7 · Cited by 7
Specialization Restricts the Evolutionary Paths Available to Yeast Sugar Transporters
Crandall, J. G., Zhou, X., Rokas, A., & Hittinger, C. T. (2024) · Molecular Biology and Evolution · doi:10.1093/molbev/msae228 · Cited by 7
Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on d-glucose and d-xylose sensing
Borgström, C., Persson, V. C., Rogova, O., Osiro, K. O., Lundberg, E., Spégel, P., & Gorwa‐Grauslund, M. F. (2022) · Microbial Cell Factories · doi:10.1186/s12934-022-01978-z · Cited by 7
D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain
Nijland, J. G., Shin, H. Y., Dore, E., Rudinatha, D., de Waal, P. P., & Driessen, A. J. M. (2020) · FEMS Yeast Research · doi:10.1093/femsyr/foaa062 · Cited by 7
Principles and practice of designing microbial biocatalysts for fuel and chemical production
Shanmugam, K. T., & Ingram, L. O. (2021) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuab016 · Cited by 7
Strategies on simultaneous fermentation of pentose and hexose to bioethanol
Zhou, M., & Lü, X. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-818862-0.00010-8 · Cited by 7
Promising approaches in the extraction, characterization, and biotechnological applications of ursolic acid: a review
Vijayalakshmi, K., Sonali, L., Kanimozhi, N. V., Pavithra, R., Drisya Raj, M. P., Suneetha, C., Raja, R., & Sukumar, M. (2025) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2025.2475094 · Cited by 6
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production
Lou, J., Wang, X., Li, R., Yao, J., Hu, J., Qin, X., Jin, M., & Yang, S. (2025) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2025.04.009 · Cited by 7
Optimizing the coordinated transcription of central xylose-metabolism genes in Saccharomyces cerevisiae
Zhang, X., Wang, J., Zhang, W., Hou, J., Xiao, W., & Cao, L. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9172-5 · Cited by 6
Hemicelluloses Role in Biorefinery Systems of Cellulosic Bioethanol, Particleboard, and Pulp and Paper Industries
Zamora, H. D. Z., Olayiwola, H. O., Jacobus, A. P., Gross, J., Tyhoda, L., & Brienzo, M. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-16-3682-0_1 · Cited by 6
Overexpression of Mutant Galactose Permease (ScGal2_N376F) Effective for Utilization of Glucose/Xylose or Glucose/ Galactose Mixture by Engineered Kluyveromyces marxianus
Kwon, D.-H., Kim, S.-B., Park, J., & Ha, S. (2020) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2008.08035 · Cited by 5
A label-free real-time method for measuring glucose uptake kinetics in yeast
Schmidl, S., Iancu, C. V., Reifenrath, M., Choe, J., & Oreb, M. (2020) · FEMS Yeast Research · doi:10.1093/femsyr/foaa069 · Cited by 5
Self-citation
Metabolic engineering of Saccharomyces cerevisiae for squalene production from lignocellulosic hydrolysates
Zhu, P., Sun, X., Fang, Y., & Ren, J. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107224 · Cited by 4
Potential Role of Xylose Transporters in Industrial Yeast for Bioethanol Production: A Perspective Review
Sharma, N. K., Behera, S., Arora, R., & Kumar, S. (2016) · Springer proceedings in energy · doi:10.1007/978-81-322-2773-1_6 · Cited by 4
Microbial activity and productivity enhancement strategies
Bhatia, S. K., Kumar, V., Kumar, V., Bhatia, R. K., & Yang, Y. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-816109-8.00004-0 · Cited by 4
Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum
Fiamenghi, M. B., Prodonoff, J. S., Borelli, G., Carazzolle, M. F., Pereira, G. A. G., & José, J. (2024) · Extremophiles · doi:10.1007/s00792-023-01327-x · Cited by 4
Metabolite transport and its impact on metabolic engineering approaches
Agrimi, G., & Steiger, M. G. (2020) · FEMS Microbiology Letters · doi:10.1093/femsle/fnaa211 · Cited by 4
Mechanistic insight into substrate specificity of plant glucosinolate transportersPreprint
Kanstrup, C., Wulff, N., Peña-Varas, C., Jørgensen, M. E., Bang-Sørensen, R., Crocoll, C., Jørgensen, F. S., Ramírez, D., Drèyer, I., Mirza, O., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.01.06.522984 · Cited by 4
Docking-guided and phosphomimetic rational engineering of glucose-insensitive xylose transporters from Trichoderma reesei
Taveira, I. C., Fier, Í., Nogueira, K. M. V., Maués, D. B., dos Santos, L. V., & Silva, R. N. (2026) · Microbial Cell Factories · doi:10.1186/s12934-026-02953-8 · Cited by 3
Engineering robust Saccharomyces cerevisiae for high-yield sabinene production from lignocellulosic hydrolysate
Meng, D., Wang, S., Li, X., & Wang, Y. (2026) · Metabolic Engineering · doi:10.1016/j.ymben.2026.03.006 · Cited by 3
A two-step mechanism for sugar translocation
Ahn, D.-H., Alleva, C., Reichenbach, T., Gulati, A., Ruda, A., Bonaccorsi, M., Silberberg, J. M., Claesson, M., Suades, A., Delemotte, L., et al. (2026) · Nature Structural & Molecular Biology · doi:10.1038/s41594-026-01784-w · Cited by 4
Improved Utility of Pentoses from Lignocellulolytic Hydrolysate: Challenges and Perspectives for Enabling Saccharomyces cerevisiae
da Silva, R. R., Prista, C., Loureiro-Dias, M. C., Boscolo, M., da Silva, R., & Gomes, E. (2019) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.9b02809 · Cited by 3
Modulated Arabinose Uptake and cAMP Signaling Synergistically Improve Glucose and Arabinose Consumption in Recombinant Yeast
Liu, J., Yang, J., Yuan, L., Wu, C., Jiang, Y., Zhuang, W., Ying, H., & Yang, S. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c04386 · Cited by 3
Enhancement of Simultaneous Xylose and Glucose Utilization by Regulating ZWF1 and PGI1 in Saccharomyces Cerevisiae
Liu, G., Li, B., Li, C., & Yuan, Y. (2017) · Transactions of Tianjin University · doi:10.1007/s12209-017-0048-z · Cited by 3
Engineering mixed sugar metabolic channels in Pseudomonas putida to produce vanillic acid
Wu, P., Ding, H., Xu, Z., Zhang, Y., Dang, Y., Gao, B., & Zhang, G. (2025) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2025.10.002 · Cited by 2
Transporter engineering in microbial cell factories
Cheng, Y., Xie, X., Guo, L., Ji, H., Madadi, M., & Qiao, Y. (2026) · Trends in biotechnology · doi:10.1016/j.tibtech.2026.06.010 · Cited by 3
Construction of the Improved Producers of First- and Second-Generation Ethanol in Conventional and Non-conventional Yeasts
Vasylyshyn, R. V., Dmytruk, K. V., Ruchała, J., & Sibirny, A. А. (2025) · Grand challenges in biology and biotechnology · doi:10.1007/978-3-031-74726-7_1 · Cited by 2
On predicting transport proteins and their substrates for the reconstruction of metabolic networks
Aplop, F., & Butler, G. (2015) · IEEE Conference on Computational Intelligence in Bioinformatics and Computational Biology (CIBCB) · doi:10.1109/cibcb.2015.7300297 · Cited by 2
Strain Improvement Methods for Enhanced Xylitol Production
Gupta, R., Mthembu, L. D., & Deenadayalu, N. (2022) · Springer eBooks · doi:10.1007/978-3-031-04942-2_4 · Cited by 2
Maltose accumulation-induced cell death in Saccharomyces cerevisiae
Zhang, X., Nijland, J. G., & Driessen, A. J. M. (2024) · FEMS Yeast Research · doi:10.1093/femsyr/foae012 · Cited by 2
A yeast-based in vivo assay system for analyzing efflux of sugars mediated by glucose and xylose transporters
Rojas, S., Boles, E., & Oreb, M. (2022) · FEMS Yeast Research · doi:10.1093/femsyr/foac038 · Cited by 2
Self-citation
The uptake of carbon sources by Aspergillus niger
Sloothaak, J. (2017) · doi:10.18174/414746 · Cited by 2
Elucidating the mutation-induced conformational changes in hexose transporter for co-utilization of glucose and xylose
Patra, P., & Ghosh, A. (2026) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2026.153302 · Cited by 1
Engineering xylose catabolism in the yeast Komagataella phaffii
Zhang, K., Ni, X., Cai, P., & Zhou, Y. J. (2026) · Metabolic Engineering · doi:10.1016/j.ymben.2026.02.001 · Cited by 1
All You Can Eat Yeast: Substituting Hexose Transporters With AtSWEET7 Alleviates Glucose Repression, Enabling Simultaneous Utilization of Sugars in Renewable Feedstocks
Kuanyshev, N., Dai, D., Kim, J., Kang, N. K., Cheng, M., Singh, V., & Jin, Y. (2026) · Biotechnology and Bioengineering · doi:10.1002/bit.70188 · Cited by 1
Engineering Saccharomyces cerevisiae for α-terpineol production from corn stover hydrolysate via UV mutagenesis and PCL1-mediated tolerance enhancement
Meng, D., Wang, S., Wang, X., Li, X., & Wang, Y. (2026) · Bioresource Technology · doi:10.1016/j.biortech.2026.135026 · Cited by 1
Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose
Donzella, L., Belloch-Molina, C., Morrissey, J. P., & Sousa, M. J. (2026) · FEMS Yeast Research · doi:10.1093/femsyr/foag035 · Cited by 1
The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum
Karapanagioti, F., Obermaier, S., Slotboom, D. J., & Poolman, B. (2025) · FEBS Letters · doi:10.1002/1873-3468.70044 · Cited by 1
Glucose receptor deletion and engineering: impact on xylose sensing and utilization in Saccharomyces cerevisiae
Bolzico, B. C., Persson, V. C., Comelli, R. N., & Gorwa‐Grauslund, M. F. (2025) · FEMS Yeast Research · doi:10.1093/femsyr/foaf040 · Cited by 1
Observation of Cellodextrin Accumulation Resulted from Non-Conventional Secretion of Intracellular β-Glucosidase by Engineered Saccharomyces cerevisiae Fermenting Cellobiose
Lee, W.-H., & Jin, Y. (2021) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2105.05018 · Cited by 1
Microbial fermentation via genetically engineered microorganisms for production of bioenergy and biochemicals
Zhou, K., & Zhou, J. F. J. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-90633-3.00015-8 · Cited by 1
Cellular Engineering of Yarrowia lipolytica for Biomanufacturing of High-Value Products from Oils and Fats
Liu, N., Soong, Y. V., Olson, A., & Xie, D. (2022) · Springer eBooks · doi:10.1007/978-3-030-89680-5_3 · Cited by 1
Non‐refined Carbon Sources and Microbial Performance
Kaur, G. (2018) · Wiley eBooks · doi:10.1002/9781119248002.ch4 · Cited by 1
Metabolic Engineering of Yeast
Pereira, R., Ishchuk, O. P., Li, X., Liu, Q., Liu, Y., Otto, M., Chen, Y., Siewers, V., & Nielsen, J. (2021) · Metabolic Engineering · doi:10.1002/9783527823468.ch18 · Cited by 1
Metabolic engineering of Saccharomyces cerevisiae for second generation ethanol from Xylo-Oligosaccharides.
Procópio, D. P. (2021) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.3.2021.tde-16032022-134640 · Cited by 1
Synthetic Yarrowia lipolytica consortium for efficient conversion of lignocellulosic oligosaccharides into lipidsPreprint
Crespo-Roche, D., Gonzalez-Garcia, D., Méndez-Líter, J. A., Ledesma‐Amaro, R., Prieto, A., & Barriuso, J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.19.695487 · Cited by 1
Engineering synthetic promoters as dynamic controllers in saccharomyces cerevisiae
Teo, W. S. (2014) · doi:10.32657/10356/61754
Engineering Hemicellulose-Derived Xylose Utilization in Saccharomyces cerevisiae for Biotechnological Applications
Malán, A. K., Fagúndez, A., Gill, P. R., & Batista, S. B. (2016) · Springer eBooks · doi:10.1007/978-981-10-2555-6_3
Systems Metabolic Engineering of Saccharomyces cerevisiae for Production of Biochemicals from Biomass
Caspeta, L., & Castillo, T. (2017) · Springer eBooks · doi:10.1007/978-3-319-51729-2_2
Development of xylose reductase isozyme system for enhancing xylose metabolism in Saccharomyces cerevisiae
조정현 (2017) · Seoul National University Open Repository (Seoul National University)
Transcriptional factor Cat8 is involved in regulation of xylose fermentation in engineered Saccharomyces cerevisiae
Dzanaieva, L. S., Dmytruk, K. V., & Sybirny, A. A. (2018) · Faktori eksperimental noi evolucii organizmiv · doi:10.7124/feeo.v22.970
Genetic Engineering of Yeast with Novel Properties Using Localized Hypermutation
Xhialli, B. (2020) · uO Research (University of Ottawa) · doi:10.20381/ruor-24765
Systematic Metabolic Engineering of Saccharomyces cerevisiae for Efficient Utilization of Xylose
Han, J., Gong, G., Wu, X., & Zha, J. (2021) · Springer eBooks · doi:10.1007/978-3-030-65584-6_7
Novel Evolutionary Engineering Approach to Alter Substrate Specificity of Disaccharide Transporter Mal11 in Saccharomyces cerevisiae
de Valk, S. C., & Mans, R. (2022) · Journal of Fungi · doi:10.3390/jof8040358
Advances in bio-manufacturing of food functional factors based on regulating cell growth of model microorganism
Bin, W. A. N. G., Lv, X., JiangHua, L. I., Long, L. I. U., Guocheng, D., Jian, C. H. E. N., & YanFeng, L. I. U. (2023) · Scientia Sinica Vitae · doi:10.1360/ssv-2023-0031
Metabolic engineering of yeast for xylose utilization via the Weimberg pathway and for xylitol production
Regmi, P. (2023) · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) · doi:10.21248/gups.74819
Influência da glicose na regulação do metabolismo de xilose em Spathaspora passalidarum
Ribeiro, L. E. (2022) · doi:10.47328/ufvbbt.2022.202
Additional file 1 of Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts
Coradetti, S. T., Adamczyk, P., Liu, D., Gao, Y., Otoupal, P. B., Geiselman, G. M., Webb‐Robertson, B., Burnet, M., Kim, Y., Burnum-Johnson, K., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849384
Additional file 1 of Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila
Li, J., Chen, B., Gu, S., Zhao, Z., Liu, Q., Sun, T., Zhang, Y., Wu, T., Liu, D., Sun, W., et al. (2021) · Figshare · doi:10.6084/m9.figshare.16674905
Additional file 2 of Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts
Coradetti, S. T., Adamczyk, P., Liu, D., Gao, Y., Otoupal, P. B., Geiselman, G. M., Webb‐Robertson, B., Burnet, M., Kim, Y., Burnum-Johnson, K., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849387
Understanding xylose transport in yeasts
Chattopadhyay, A., Mitra, M., & Maiti, M. K. (2024) · Vitamins and hormones · doi:10.1016/bs.vh.2024.10.005
Caracterização de genes oriundos de Candida tropicalis potencialmente envolvidos no transporte de xilose
Queiroz, S. D. S. (2023) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.97.2023.tde-11122024-154626
Additional file 6 of Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast
Fiamenghi, M. B., Bueno, J. G. R., Camargo, A. P., Borelli, G., Carazzolle, M. F., Pereira, G. A. G., dos Santos, L. V., & José, J. (2022) · Figshare · doi:10.6084/m9.figshare.19808014
Additional file 2 of Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
Vasylyshyn, R. V., Kurylenko, O., Ruchala, J., Shevchuk, N., Kuliešienė, N., Khroustalyova, G., Rapoport, A. I., Daugelavičius, R., Kostyantyn, D., & Sibirny, A. (2020) · Figshare · doi:10.6084/m9.figshare.12197541
Additional file 1 of Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
Vasylyshyn, R. V., Kurylenko, O., Ruchala, J., Shevchuk, N., Kuliešienė, N., Khroustalyova, G., Rapoport, A. I., Daugelavičius, R., Dmytruk, K., & Sibirny, A. (2020) · Figshare · doi:10.6084/m9.figshare.12197538
Additional file 3 of Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts
Coradetti, S. T., Adamczyk, P. A., Liu, D., Gao, Y., Otoupal, P. B., Geiselman, G. M., Webb-Robertson, B.-J. M., Burnet, M. C., Kim, Y.-M., Burnum-Johnson, K. E., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849393
Additional file 2 of Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila
Li, J., Chen, B., Gu, S., Zhao, Z., Liu, Q., Sun, T., Zhang, Y., Wu, T., Liu, D., Sun, W., et al. (2021) · Figshare · doi:10.6084/m9.figshare.16674908
Additional file 1 of d-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae
Nijland, J. G., Zhang, X., & Driessen, A. J. M. (2024) · Figshare · doi:10.6084/m9.figshare.26583006
Additional file 1 of Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei
Havukainen, S., Pujol‐Giménez, J., Valkonen, M. J., Hediger, M. A., & Landowski, C. P. (2021) · Figshare · doi:10.6084/m9.figshare.16590803
Additional file 7 of Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast
Fiamenghi, M. B., Bueno, J. G. R., Camargo, A. P., Borelli, G., Carazzolle, M. F., Pereira, G. A. G., dos Santos, L. V., & José, J. (2022) · Figshare · doi:10.6084/m9.figshare.19808017
Using wheat-rye 1BL.1RS translocations to disclose the effects of subtelomeric tandem repeats on meiotic behavior of homologues
Zou, Y., Xiong, Z., Yang, J., Lv, H., Guo, J., Fu, S., & Tang, Z. (2026) · BMC Plant Biology · doi:10.1186/s12870-025-08088-z
Current status and new avenues for first- and second-generation ethanol: Integrating processes and yeasts
dos Santos, Â. A., Rüntzel, C. L., Klanovicz, N., Minussi, G. D. A., Schutz, D., da Fontoura, I. C. C., Treichel, H., Stambuk, B. U., & Alves, S. L. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-36567-6.00008-0
Reprogramming xylose metabolism for sustainable cadaverine production in engineered Escherichia coliPreprint
Shan, G., Zhao, Y., Han, H., Xue, B., Wang, S., & Su, H. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.6588268
Functional characterization of sugar transporters in Candida tropicalis using Saccharomyces cerevisiae as a host
Jiang, L., Zhang, S., Zhang, Z.-W., Ali, S., Shen, J., Xin, W., Feng, L., Hu, J., & Ma, M. (2026) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2026.106355
Progresses of transposon research in Saccharomyces cerevisiae
Lin, W., Lv, Y., Zhang, Y., Jin, Z., Liu, L., & Zhang, D. (2026) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2026.05.004
Ashbya gossypii : From Riboflavin Workhorse to a Next-Generation Cell Factory
Jiménez, A., López-Rubio, M., Martín‐González, J., Hernández‐Gómez, A., & Buey, R. M. (2026) · Critical Insights in Biotechnology · doi:10.1080/29932238.2026.2673683
Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology
Bueno, J. G. R., Fier, Í., Kell, D. B., & dos Santos, L. V. (2026) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2026.2680416
Genetic Improvement of a Wild-Type Saccharomyces cerevisiae Strain for Enhanced Xylitol Production
Lagos, M. A. P., Pinzón, D. C. T., Caviativa, J. A. C., Velásquez-Lozano, M. E., & Stambuk, B. U. (2026) · Fermentation · doi:10.3390/fermentation12080354
Metabolic Engineering of Saccharomyces cerevisiae for Glucaric Acid Production from Rice Straw HydrolysatePreprint
Sun, J., Wu, A., Ma, W., Shen, F., 吳竹松, Yin, D., Liu, Q., & Zhu, T. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7207394
Genome-scale overexpression screening identifies product tolerance and efflux transport as key determinants of high-level L-tryptophan production in Escherichia coli
Jiang, Y., Zhou, Q., Xu, M., Meng, G., Zhao, C., Wei, A., Wang, Z., 贾慧萍, Yang, J., Liu, J., et al. (2026) · Metabolic Engineering · doi:10.1016/j.ymben.2026.102547
Engineering the acid-tolerant yeast Issatchenkia orientalis for efficient lactic acid production via optimized glucose and xylose utilization
Lin, Y., Kim, A., Lee, D., & Kang, N. K. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1951033
Advances in Engineered Strain Development to Improve the Carbon Atom Economy of Lignocellulosic Bioethanol Production
Li, Y., Gao, Y., Wu, Y., Liu, Y., Zhao, P., Zhao, S., Ji, H., Zhang, H., Qin, P., & Cai, D. (2026) · Green Carbon · doi:10.1016/j.greenca.2026.07.005

Authors: Farwick, A., Bruder, S., Schadeweg, V., Oreb, M., & Boles, E.
Affiliations: Goethe University Frankfurt (DE)
ORCID: Farwick, Alexander
Bruder, Stefan
Oreb, Mislav
Boles, Eckhard
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2014-03-24
Volume: 111
Issue: 14
Pages: 5159-5164
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1323464111
PMC: PMC3986176
OpenAlex: W2118069954
Cited by: 298
References: 54
Citations by year: 2026: 20 · 2025: 12 · 2024: 17 · 2023: 20 · 2022: 25 · 2021: 35 · 2020: 28 · 2019: 26 · 2018: 26 · 2017: 35 · 2016: 26 · 2015: 22 · 2014: 6
FWCI: 12.16
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: hexose transporters, GAL2, XylE, sugar specificity, competitive inhibition, lignocellulosic biomass, Saccharomyces cerevisiae
MeSH terms: Biological Transport, Glucose, Hydrolysis, Models, Molecular, Monosaccharide Transport Proteins, Saccharomyces cerevisiae, Xylose, Protein Engineering, Mutagenesis, Site-Directed

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Engineering of yeast hexose transporters to transport d -xylose without inhibition by d -glucose

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks