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Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix
Open accessAbstract
Significance: Exopolysaccharides and extracellular DNA are important structural components that contribute to the self-assembly of large aggregates or microcolonies that are characteristic of biofilms. Pseudomonas aeruginosa is capable of producing multiple exopolysaccharides, including alginate, Psl, and Pel. At present, little is known about Pel’s chemical structure and its role in microcolony formation. Our results demonstrate that Pel is composed of cationic amino sugars. Using this knowledge, we have developed a Pel-specific lectin stain to directly visualize Pel in biofilms. We show that the positive charge on Pel facilitates its binding to extracellular DNA in the biofilm stalk, and that Pel can compensate for lack of Psl in the biofilm periphery.
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Cited by
Biofilms: an emergent form of bacterial life
Flemming, H.-C., Wingender, J., Szewzyk, U., Steinberg, P., Rice, S. A., & Kjelleberg, S. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.94 · Cited by 5,979
Gunn, J. S., Bakaletz, L. O., & Wozniak, D. J. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.707547 · Cited by 211
Self-citationVandana, & Das, S. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119536 · Cited by 199
Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity
Snarr, B. D., Baker, P., Bamford, N. C., Sato, Y., Liu, H., Lehoux, M., Gravelat, F. N., Ostapska, H., Baistrocchi, S. R., Cerone, R. P., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1702798114 · Cited by 95
Self-citationBiofilms deform soft surfaces and disrupt epithelia
Cont, A., Rossy, T., Al-Mayyah, Z., & Persat, A. (2020) · eLife · doi:10.7554/elife.56533 · Cited by 70
Metadata
- Authors
- Jennings, L. K., Storek, K. M., Ledvina, H. E., Coulon, C., Marmont, L. S., Sadovskaya, I., Secor, P. R., Tseng, B. S., Scian, M., Filloux, A., Wozniak, D. J., Howell, P. L., & Parsek, M. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-08-26
- Volume
- 112
- Issue
- 36
- Pages
- 11353-11358
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2124439712
- Cited by
- 624
- References
- 0
- FWCI
- 14.80
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix. https://app.cochat.ai/papers/10.1073/pnas.1503058112
- The paper
- Jennings, L. K., Storek, K. M., Ledvina, H. E., Coulon, C., Marmont, L. S., Sadovskaya, I., Secor, P. R., Tseng, B. S., Scian, M., Filloux, A., Wozniak, D. J., Howell, P. L., & Parsek, M. R. (2015). Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1503058112
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
