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Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

Bardy, Cedric, van den Hurk, Mark, Eames, Tameji, Marchand, Cynthia, Hernandez, Ruben V., Kellogg, Mariko, Gorris, Mark, Galet, Ben +8 more·2015·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1504393112
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Abstract

Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiological medium also improved the proportion of neurons that were synaptically active. The medium was designed to culture human neurons but also proved adequate for rodent neurons. The improvement in BrainPhys basal medium to support neurophysiological activity is an important step toward reducing the gap between brain physiological conditions in vivo and neuronal models in vitro.

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Authors
Bardy, C., van den Hurk, M., Eames, T., Marchand, C., Hernandez, R. V., Kellogg, M., Gorris, M., Galet, B., Palomares, V., Brown, J., Bang, A. G., Mertens, J., Böhnke, L., Boyer, L., Simon, S., & Gage, F. H.
Affiliations
  • Salk Institute for Biological Studies, Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037;
  • Conrad Prebys Center for Chemical Genomics, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2015-04-13
Volume
112
Issue
20
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.1504393112
Cited by
428
References
7
Language
en
Update notices
Licence
www.pnas.org/site/misc/userlicense.xhtml (vor)
Funders
  • Marie Curie International Outgoing Fellowship For Career Development (HND (FP7-IOF))
  • IPSEN PHARMA S.A.S (-)
  • Annette C. Merle-Smith (-)
  • The Leona M. and Harry B. Hemsley Charitable Trust (#2012-PG-MED002)
  • Bob and Mary Jane Engman (-)
  • JPB Foundation (#113)
  • LGMD2i Research Fund, Erna and Andrew Viterbi and the Viterbi Family of the Jewish Community Foundation San Diego Neuroscience Research Initiative (-)
  • G Harold and Leila Y. Mathers Foundation (-)
  • HHS | NIH | National Institute of Mental Health (MH095741)

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