The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness
Open accessAbstract
Significance: Human cardiomyocytes differentiated from pluripotent stem cells (hPSC-CMs) have potential as in vitro models of cardiac health and disease but differ from mature cardiomyocytes. In single live engineered hPSC-CMs with physiological shapes, we assayed the mechanical output and activity of sarcomeres and myofibrils in a nondestructive, noninvasive manner. Substrates with physiological stiffness improved contractile activity of patterned hPSC-CMs, as well as calcium flow, mitochondrial organization, electrophysiology, and transverse-tubule formation. The mechanical output and activity of sarcomeres and myofibrils varied as a function of mechanical cues and disrupted cell tension. This study establishes a high-throughput platform for modeling single-cell cardiac contractile activity and yields insight into environmental factors that drive maturation and sarcomere function in hPSC-CMs.
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Cites
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
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Engineering Adolescence: Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes
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Actin stress fibers – assembly, dynamics and biological roles
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Small and large animal models in cardiac contraction research: advantages and disadvantages
Milani-Nejad, N., & Janssen, P. M. L. (2014) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2013.10.007 · Cited by 484
Differential expression of cardiac titin isoforms and modulation of cellular stiffness
Cazorla, O., Freiburg, A., Helmes, M., Centner, T., McNabb, M., Wu, Y., Trombitás, K., Labeit, S., & Granzier, H. (2000) · Circulation Research · doi:10.1161/01.res.86.1.59 · Cited by 418
Cited by
Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine
Karbassi, E., Fenix, A., Marchiano, S., Muraoka, N., Nakamura, K., Yang, X., & Murry, C. E. (2020) · Nature Reviews Cardiology · doi:10.1038/s41569-019-0331-x · Cited by 755
Bioengineering strategies to accelerate stem cell therapeutics
Madl, C. M., Heilshorn, S. C., & Blau, H. M. (2018) · Nature · doi:10.1038/s41586-018-0089-z · Cited by 425
Sala, L., van Meer, B. J., Tertoolen, L. G. J., Bakkers, J., Bellin, M., Davis, R. P., Denning, C., Dieben, M. A. E., Eschenhagen, T., Giacomelli, E., et al. (2018) · Circulation Research · doi:10.1161/circresaha.117.312067 · Cited by 416
Ma, X., Liu, J., Zhu, W., Tang, M., Lawrence, N., Yu, C., Gou, M., & Chen, S. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.06.011 · Cited by 401
Tuning the Range of Polyacrylamide Gel Stiffness for Mechanobiology Applications
Denisin, A. K., & Pruitt, B. L. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b09344 · Cited by 313
Self-citation
Metadata
- Authors
- Ribeiro, A. J. S., Ang, Y.-S., Fu, J.-D., Rivas, R. N., Mohamed, T. M. A., Higgs, G. C., Srivastava, D., & Pruitt, B. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-09-28
- Volume
- 112
- Issue
- 41
- Pages
- 12705-12710
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2200325631
- Cited by
- 479
- References
- 6
- FWCI
- 13.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Ribeiro, A. J. S., Ang, Y.-S., Fu, J.-D., Rivas, R. N., Mohamed, T. M. A., Higgs, G. C., Srivastava, D., & Pruitt, B. L. (2015). Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1508073112
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
