The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Synthetic CRISPR RNA-Cas9–guided genome editing in human cells
Open accessAbstract
Significance: Genome editing with nucleases that recognize specific DNA sequences is a powerful technology for manipulating genomes. This is especially true for the Cas9 nuclease, the site specificity of which is determined by a bound RNA, called a CRISPR RNA (crRNA). Here we develop a chemically modified, 29-nucleotide synthetic CRISPR RNA (scrRNA) and show that it can functionally replace the natural crRNA, producing enhanced cleavage activity at a target DNA site with apparently reduced off-target cleavage. scrRNAs can be synthesized in a commercially feasible manner today and provide a platform for therapeutic applications.
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Citations
Cites
RNA-Guided Human Genome Engineering via Cas9
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DNA targeting specificity of RNA-guided Cas9 nucleases
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High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
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Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.029 · Cited by 2,728
In vivo genome editing using Staphylococcus aureus Cas9
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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
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“It has been previously shown that CRISPR-Cas9 specificity can be enhanced by reduction of the 20-nucleotide DNA specificity sequence to 17 nucleotides without affecting overall ontarget activity (36).”
BackgroundInfluentialRNA-guided genetic silencing systems in bacteria and archaea
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Cited by
Applications of CRISPR technologies in research and beyond
Barrangou, R., & Doudna, J. A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3659 · Cited by 1,005
Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
Yin, H., Song, C.-Q., Suresh, S., Wu, Q., Walsh, S., Rhym, L. H., Mintzer, E., Bolukbasi, M. F., Zhu, L. J., Kauffman, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4005 · Cited by 499
DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing
Xue, C., & Greene, E. C. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.02.008 · Cited by 431
Genome-Editing Technologies: Principles and Applications
Gaj, T., Sirk, S. J., Shui, S.-L., & Liu, J. (2016) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023754 · Cited by 381
Metadata
- Authors
- Rahdar, M., McMahon, M. A., Prakash, T. P., Swayze, E. E., Bennett, C. F., & Cleveland, D. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-11-16
- Volume
- 112
- Issue
- 51
- Pages
- E7110-7
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2175485670
- Cited by
- 186
- References
- 15
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- green
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- Rahdar, M., McMahon, M. A., Prakash, T. P., Swayze, E. E., Bennett, C. F., & Cleveland, D. W. (2015). Synthetic CRISPR RNA-Cas9–guided genome editing in human cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1520883112
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