The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging
Open accessAbstract
Significance: Biofilms are surface-associated bacterial communities embedded in an extracellular matrix. Connections between biofilm architectural, material, and mechanical features have never been systematically studied at the individual cell level due to inadequate optical resolution. Here, we develop imaging, experimental, and modeling tools to analyze living, growing bacterial biofilms at single-cell resolution. We discover that Vibrio cholerae biofilms undergo a 2D-to-3D transition as a consequence of directional cell division and anisotropic pressure caused by cell-to-surface adhesion. Moreover, deletion of a single gene responsible for cell-to-cell adhesion changes the biofilm growth mode from directional cell growth to expansion caused by the extracellular matrix. The technology reported here enables future studies of single-cell gene expression in bacterial communities.
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Hobley, L., Harkins, C., MacPhee, C. E., & Stanley-Wall, N. R. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv015 · Cited by 581 · cited 2×
“Matrix proteins, extracellular DNA, lipids, and bacteriophages have also been shown to influence the formation of the overall biofilm structure (8).”
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Cited by
Surviving as a Community: Antibiotic Tolerance and Persistence in Bacterial Biofilms
Yan, J., & Bassler, B. L. (2019) · Cell Host & Microbe · doi:10.1016/j.chom.2019.06.002 · Cited by 747
Self-citationA Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision
Silpe, J. E., & Bassler, B. L. (2019) · Cell · doi:10.1016/j.cell.2018.10.059 · Cited by 363
Self-citationEmergence of three-dimensional order and structure in growing biofilms
Hartmann, R., Singh, P. K., Pearce, P., Mok, R., Song, B., Díaz-Pascual, F., Dunkel, J., & Drescher, K. (2019) · Nature Physics · doi:10.1038/s41567-018-0356-9 · Cited by 283
Dynamic biofilm architecture confers individual and collective mechanisms of viral protection
Vidakovic, L., Singh, P. K., Hartmann, R., Nadell, C. D., & Drescher, K. (2017) · Nature Microbiology · doi:10.1038/s41564-017-0050-1 · Cited by 265
Biofilms deform soft surfaces and disrupt epithelia
Cont, A., Rossy, T., Al-Mayyah, Z., & Persat, A. (2020) · eLife · doi:10.7554/elife.56533 · Cited by 70
Metadata
- Authors
- Yan, J., Sharo, A. G., Stone, H. A., Wingreen, N. S., & Bassler, B. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2016-08-23
- Volume
- 113
- Issue
- 36
- Pages
- E5337-43
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2507231691
- Cited by
- 161
- References
- 1
- FWCI
- 7.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yan, J., Sharo, A. G., Stone, H. A., Wingreen, N. S., & Bassler, B. L. (2016). Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1611494113
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