Cited by 202Neuroscience and Neuropharmacology ResearchRNA Research and SplicingRNA and protein synthesis mechanisms

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Astrocytes locally translate transcripts in their peripheral processes

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Sakers, Kristina, Lake, Allison M., Khazanchi, Rohan, Ouwenga, Rebecca, Vasek, Michael J., Dani, Adish, Dougherty, Joseph D.·2017·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1617782114PMC PMC5441704

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Abstract

Significance: Cellular compartments are specialized for particular functions. In astrocytes, the peripheral, perisynaptic processes contain proteins specialized for reuptake of neurotransmitters and ions, and have been shown to alter their morphology in response to activity. Regulated transport of a specific subset of nuclear-derived mRNAs to specific compartments is thought to support the specialization of these compartments and allow for local regulation of translation. In neurons, local translation near activated synapses is thought to generate the proteins needed for the synaptic alterations that constitute memory. We demonstrate that astrocytes also have sequence-dependent local translation in their peripheral processes, including transcripts with roles in regulating synapses, and identify one mechanism regulating this translation. These findings suggest local translation in astrocyte processes may play a role in synapse modulation.

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“From the Dougherty lab’s earlier work, we have learned that translation exists in distal astrocytic processes (Sakers et al., 2017), and that line of research has been extended to microglia (Vasek et al.”
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“Given that local mRNA translation occurs in axons 65 , dendrites 71 and astrocytic processes 72 , and that we were imaging transcripts in 2 dimensions on tissue sections that were 6 µm thick, it is simply not possible to definitively assign any individual transcript to a specific cell with a high…”
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“…to our knowledge, all published mammalian localized and synaptic transcriptomic datasets derived from multiple types of glia (Thomsen et al., 2013; Thakurela et al., 2016; Boulay et al., 2017; Sakers et al., 2017; Azevedo et al., 2018; Mazaré et al., 2020; Vasek et al., 2021), outlined in Table 4.”
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“8) 15 .”
InfluentialSelf-citation
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“…in the imaging field excluding the astrocytic ROIs (astrocytic n = 507 and non-astrocytic n = 4823 blinking events from 11 images from 3 animals, two-sample low-copy transcripts may also remain undetected, particularly those that are locally translated in distal processes (Sakers et al. 2017).”
Condensates on the Move: Midbody Remnants as Large, Translation‐Competent Extracellular Vesicles
Skop, A. R. (2026) · BioEssays · doi:10.1002/bies.70168
“This idea is consistent with the broader importance of local translation in the nervous system [84, 85, 50], although no direct evidence currently demonstrates that MBRs play a defined role in synaptic plasticity, learning, repair, or degeneration.”

Authors: Sakers, K., Lake, A. M., Khazanchi, R., Ouwenga, R., Vasek, M. J., Dani, A., & Dougherty, J. D.
Affiliations: Washington University in St. Louis (US)
Corresponding author: Dougherty, Joseph D.
ORCID: Sakers, Kristina
Lake, Allison M.
Khazanchi, Rohan
Ouwenga, Rebecca
Vasek, Michael J.
Dani, Adish
Dougherty, Joseph D.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2017-04-24
Volume: 114
Issue: 19
Pages: E3830-E3838
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1617782114
PMC: PMC5441704
OpenAlex: W2951270532
Cited by: 202
References: 72
Citations by year: 2026: 24 · 2025: 17 · 2024: 20 · 2023: 25 · 2022: 22 · 2021: 27 · 2020: 20 · 2019: 20 · 2018: 19 · 2017: 8
FWCI: 2.26
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.pnas.org/site/misc/userlicense.xhtml (vor, 183 d)
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Children's Discovery Institute (MD-II-2013-269)
HHS | National Institutes of Health (DA038458-01, MH099798-01)
WUSTL Interface of Psychology Neuroscience and Genetics Training Grant (5T32 GM081739)
WUSTL Neurosciences Program Training Grant (T32 GM008151)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords: astrocytes, local translation, mRNA transport, synaptic regulation
MeSH terms: Animals, Astrocytes, Cell Nucleus, Humans, Memory, Neurons, RNA, Messenger, Synapses, Protein Biosynthesis

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Astrocytes locally translate transcripts in their peripheral processes

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