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Astrocytes locally translate transcripts in their peripheral processes
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Abstract
Significance: Cellular compartments are specialized for particular functions. In astrocytes, the peripheral, perisynaptic processes contain proteins specialized for reuptake of neurotransmitters and ions, and have been shown to alter their morphology in response to activity. Regulated transport of a specific subset of nuclear-derived mRNAs to specific compartments is thought to support the specialization of these compartments and allow for local regulation of translation. In neurons, local translation near activated synapses is thought to generate the proteins needed for the synaptic alterations that constitute memory. We demonstrate that astrocytes also have sequence-dependent local translation in their peripheral processes, including transcripts with roles in regulating synapses, and identify one mechanism regulating this translation. These findings suggest local translation in astrocyte processes may play a role in synapse modulation.
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“signifying local translation, a scenario described for Gfap and other key astrocyte genes35, 36.”
From Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer’s disease (2024) · cites this paper as [35]
Experimental — accuracy not yet measuredIn the citing paper
“We further confirmed that HA-tagged ribosomes are present throughout the astrocyte, including distal processes where local translation occurs (Figure 1G; Sakers et al., 2017).”
From The aging astrocyte transcriptome from multiple regions of the mouse brain. (2018) · cites this paper as Sakers et al., 2017
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“Therefore, the spatial arrangement of PAPs relative to the synapse (for instance distance or astrocyte surface per volume) is expected to shape perisynaptic glutamate signaling as much as the transporter density in these PAPs.”
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Spatially resolved single-cell translatomics at molecular resolution
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Mattioli, C. C., Rom, A., Franke, V., Imami, K., Arrey, G., Terne, M., Woehler, A., Akalin, A., Ulitsky, I., & Chekulaeva, M. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky1270 · Cited by 125
“Interestingly, among identified Cdc42E7-bound RBPs is Quaking (QKI), previously suggested to play a role in RNA localization and translation in perisynaptic astrocyte processes (43), and polypyrimidine tract binding protein 2 (PTBP2), which is involved in Cdc42 splicing (20).”
BackgroundA Paranigral VTA Nociceptin Circuit that Constrains Motivation for Reward
Parker, K. E., Pedersen, C. E., Gomez, A. M., Spangler, S. M., Walicki, M. C., Feng, S., Stewart, S., Otis, J. M., Al‐Hasani, R., McCall, J. G., et al. (2019) · Cell · doi:10.1016/j.cell.2019.06.034 · Cited by 113
“We validated TRAP isolation with robust enrichment of Pnoc as well as a host of other GPCR and neuropeptide mRNAs in these neurons (Figures 4N and S4N; Table S2).”
MethodInfluentialSelf-citationSuper-resolving Microscopy in Neuroscience
Werner, C. D., Sauer, M., & Geis, C. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01174 · Cited by 113
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“We marked the site of injection using AAV9 expressing cMyc-tagged Cyan Fluorescent Protein (CFP) under the Gfap promoter (Sakers et al., 2017).”
MethodSelf-citationMartins, D., Giacomel, A., Williams, S., Turkheimer, F., Dipasquale, O., & Veronese, M. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.110173 · Cited by 97
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“Moreover, recent work has shown that local translation also occurs in other classes of cells, such as astrocytes (Boulay et al., 2017; Sakers et al., 2017) and GABAergic neurons (Ouwenga et al.”
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Lia, A., Henriques, V. J., Zonta, M., Chiavegato, A., Carmignoto, G., Gómez‐Gonzalo, M., & Losi, G. (2021) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2021.673433 · Cited by 105
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“Indeed, the previous attempt to characterize PAP ribosome-bound mRNAs used indirect subtractive approaches to remove contaminating neuronal mRNAs, which suggested that only astrocyte-enriched or astrocyte-specific transcripts were considered (Sakers et al., 2017).”
InfluentialNo Longer Underappreciated: The Emerging Concept of Astrocyte Heterogeneity in Neuroscience
Pestana, F., Edwards‐Faret, G., Belgard, T. G., Martirosyan, A., & Holt, M. G. (2020) · Brain Sciences · doi:10.3390/brainsci10030168 · Cited by 91
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BackgroundDistribution and Function of the Bestrophin-1 (Best1) Channel in the Brain
Oh, S., & Lee, C. J. (2017) · Experimental Neurobiology · doi:10.5607/en.2017.26.3.113 · Cited by 77
“Perhaps the inconsistency is came from the fact that astrocytes locally translate some of the gene transcripts such as Best1 mRNA preferentially in the distal processes and microdomains as recently described [23].”
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Shan, L., Zhang, T., Fan, K. S., Cai, W., & Liu, H. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.680301 · Cited by 77
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“The glutamate transporters that are locally synthesized at PAPs are inserted into the PAP membrane for glutamate uptake.”
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Forrest, S. L., Kril, J. J., & Halliday, G. M. (2019) · Acta Neuropathologica · doi:10.1007/s00401-019-02035-7 · Cited by 74
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“Interestingly, EAATs 1 and 2 can be synthesized locally in the astrocytic processes [30], which allows for the fast upregulation of glutamate reuptake if necessary.”
BackgroundTranscriptomic Analysis of Ribosome-Bound mRNA in Cortical Neurites In Vivo
Ouwenga, R., Lake, A. M., O'Brien, D., Mogha, A., Dani, A., & Dougherty, J. D. (2017) · Journal of Neuroscience · doi:10.1523/jneurosci.3044-16.2017 · Cited by 65
“Using a variation of the SynapTRAP approach we have also now just shown that astrocytes also have potential for local translation as well (Sakers et al., 2017).”
MethodSelf-citationDroplet-based transcriptome profiling of individual synapses
Niu, M., Cao, W., Wang, Y., Zhu, Q., Luo, J., Wang, B., Zheng, H., Weitz, D. A., & Zong, C. C. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01635-1 · Cited by 62
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“Similar to neurons, mammalian protoplasmic astrocytes carry out local translation of mRNA in their fine processes (82), raising the question of whether astrocytic fine processes may act as semiautonomous subdomains when they interact with synapses or neural circuits.”
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Holt, M. G. (2023) · Essays in Biochemistry · doi:10.1042/ebc20220136 · Cited by 60
Aqp4 stop codon readthrough facilitates amyloid-β clearance from the brain
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Self-citationAstrocyte structural heterogeneity in the mouse hippocampus
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“Similar to what has been reported for neurons (100), local translation of transcripts in the peripheral processes of astrocytes has recently been described (101).”
ResultSubcellular and regional localization of mRNA translation in midbrain dopamine neurons
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“Astrocytic processes containing mRNA and ribosomes are also present in synaptosome preparations (Chicurel et al., 1993; Mazaré et al., 2020; Sakers et al., 2017) and are a likely source of mRNA in our FASS samples.”
Activity-dependent translation dynamically alters the proteome of the perisynaptic astrocyte process
Sapkota, D., Kater, M. S. J., Sakers, K., Nygaard, K. R., Liu, Y., Koester, S. K., Fass, S. B., Lake, A. M., Khazanchi, R., Khankan, R. R., et al. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111474 · Cited by 49
“Finally, regulation of translation allows localized production of protein in specific subcellular compartments, such as near synapses, as observed in neurons (Cajigas et al., 2012; Hafner et al., 2019; Ouwenga et al., 2017, 2018; Steward et al., 2015), and in peripheral processes of astroglia, including the perisynapt…”
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Astrocyte-synapse interactions during brain development
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The Quaking RNA ‐binding proteins as regulators of cell differentiation
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“are locally translated at the peripheral processes and that these mRNAs had higher occurrence of the QKI motif in their 30 UTRs, with QKI-6 binding many of these mRNAs (Sakers et al., 2017; Sakers et al., 2021).”
BackgroundThe Nanoworld of the Tripartite Synapse: Insights from Super-Resolution Microscopy
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“Interestingly, Plxnb1 mRNA puncta were not only detected in cell somas, but also in hypertrophic processes of reactive astrocytes, signifying local translation, which has been also described for Gfap and other key astrocyte genes 35,36 .”
Astrocyte Molecular Clock Function in the Nucleus Accumbens Is Important for Reward-Related Behavior
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Astrocytes functionally integrate multiple synapses via specialized leaflet domains
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Translational control in aging and neurodegeneration
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“A study applying TRAP in Astrocytes identified local translation in the peripheral processes of these cells that could alter adjacent synapses (Sakers et al., 2017).”
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Georgiou, L., Echeverría, A., Georgiou, A., & Kühn, B. (2022) · Science Advances · doi:10.1126/sciadv.abe5371 · Cited by 38
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Essential Role of Astrocytes in Learning and Memory
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“More in detail, these molecules are synthesized in PAPs, near the synapses where they will be released [32].”
BackgroundDisruption of Glutamate Transport and Homeostasis by Acute Metabolic Stress
Passlick, S., Rose, C. R., Petzold, G. C., & Henneberger, C. (2021) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2021.637784 · Cited by 39
“Intriguingly, it was also shown more recently that EAAT1 and EAAT2 mRNAs are found in astrocyte processes along with the machinery for local translation and that the distribution of mRNAs was altered after fear conditioning (Sakers et al., 2017; Mazaré et al., 2020).”
BackgroundThe Expanding Toolkit of Translating Ribosome Affinity Purification
Dougherty, J. D. (2017) · Journal of Neuroscience · doi:10.1523/jneurosci.1929-17.2017 · Cited by 37
“It was also clear that non-neuronal cells contributed to these biochemical fractions as we discovered sequence-specific localized translation in astrocytes as well (Sakers et al., 2017).”
BackgroundSelf-citationmRNA Transport and Local Translation in Glia
Meservey, L. M., Topkar, V. V., & Fu, M. (2021) · Trends in Cell Biology · doi:10.1016/j.tcb.2021.03.006 · Cited by 37
Local translation in microglial processes is required for efficient phagocytosis
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GFAP-isoforms in the nervous system: Understanding the need for diversity
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Imaging tripartite synapses using super-resolution microscopy
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“For example, local translation of mRNA in astrocytic processes is suggested to be important for shaping the repertoire of astrocytic plasma membrane and secreted proteins warranting support to neurons [14, 15].”
BackgroundAstrocytic TRPV4 Channels and Their Role in Brain Ischemia
Turečková, J., Heřmanová, Z., Marchetti, V., & Anděrová, M. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087101 · Cited by 34
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Vitronectin from brain pericytes promotes adult forebrain neurogenesis by stimulating CNTF
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“Moreover, astrocytes can translate transcripts in their peripheral processes (Sakers et al., 2017), raising the possibility that pericytes affect astrocyte protein expression locally, in addition to retrograde signaling to their nuclei, in order to regulate neurogenesis.”
Díaz, A., Merino, P., Manrique, L. G., Cheng, L., & Yepes, M. (2018) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x18783653 · Cited by 30
“Astrocytes interact with the synapse via highly motile elongations known as PAPs.20 Significantly, although the interaction of PAPs with the synapse is crucial for the processing of neuronal information,34 induction of synaptic plasticity,41 synapse formation and maturation,42 and maintenance of synaptic stability,4 t…”
BackgroundResultInfluentialThe computational power of the human brain
Gebicke‐Haerter, P. J. (2023) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2023.1220030 · Cited by 35
“Ensheathment of synaptic spines by perisynaptic astrocytic processes (PAPs) can change over time.”
BackgroundInfluentialAstrocyte diversity and subtypes: aligning transcriptomics with multimodal perspectives
Hennes, M., Richter, M. L., Fischer-Sternjak, J., & Götz, M. (2025) · EMBO Reports · doi:10.1038/s44319-025-00529-y · Cited by 35
“Moreover, they also locally translate cytoskeletal proteins possibly involved in morphological remodeling of their processes (Sakers et al, 2017).”
AstroDot – a new method for studying the spatial distribution of mRNA in astrocytes
Oudart, M., Tortuyaux, R., Mailly, P., Mazaré, N., Boulay, A.-C., & Cohen‐Salmon, M. (2020) · Journal of Cell Science · doi:10.1242/jcs.239756 · Cited by 32
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“Our recent refinement of the TRAP protocol now makes it possible to compare ribosome-bound mRNAs in PAPs and PvAPs from a given region of the brain (Mazaré et al., 2020).”
BackgroundMethodInfluentialBlanco‐Urrejola, M., Gaminde‐Blasco, A., Gamarra, M., de la Cruz, A., Vecino, E., Alberdi, E. M., & Baleriola, J. (2021) · Cells · doi:10.3390/cells10030632 · Cited by 24
Cellular neurometabolism: a tentative to connect cell biology and metabolism in neurology
García‐Cazorla, À., & Saudubray, J. (2018) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-018-0226-8 · Cited by 28
“Additionally there is an endosome-free route through mRNA binding proteins for local translation (Boulay et al. 2017; Sakers et al. 2017).”
BackgroundDvorzhak, A., Helassa, N., Török, K., Schmitz, D., & Grantyn, R. (2019) · Journal of Neuroscience · doi:10.1523/jneurosci.2865-18.2019 · Cited by 27
Arizono, M., & Nägerl, U. V. (2021) · Glia · doi:10.1002/glia.24103 · Cited by 28
“The super-resolution technique STORM was recently used to reveal nanoscale distributions of ribosomal proteins and glutamate transporters in astrocytes (Heller et al., 2020; Sakers et al., 2017).”
MethodHeterogeneity of the astrocytic AMPA‐receptor transcriptome
Mölders, A., Koch, A., Menke, R., & Klöcker, N. (2018) · Glia · doi:10.1002/glia.23514 · Cited by 27
“Dissociated cells were harvested by centrifugation and cells of 3–4 littermates were pooled for FACS.”
BackgroundInfluentialIntracellular spatial transcriptomic analysis toolkit (InSTAnT)
Kumar, A., Schrader, A. W., Aggarwal, B., Ebrahimpour-Boroojeny, A., Asadian, M., Lee, J., Song, Y. J., Zhao, S. D., Han, H., & Sinha, S. (2024) · Nature Communications · doi:10.1038/s41467-024-49457-w · Cited by 26
Local gene regulation in radial glia: Lessons from across the nervous system
D’Arcy, B. R., & Silver, D. L. (2020) · Traffic · doi:10.1111/tra.12769 · Cited by 22
“To expand upon this discovery and identify transcripts likely to be locally translated, the authors performed in-depth characterization of ribosome-bound transcripts in PAPs.107 To do so, they developed and employed the PAP-TRAP method, similar to other TRAP methods, to isolate and sequence ribosome-bound mRNAs in ast…”
BackgroundMethodInfluentialDe novo proteomic methods for examining the molecular mechanisms underpinning long-term memory
Evans, H. T., Blackmore, D. G., Götz, J., & Bodea, L. (2021) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2020.12.015 · Cited by 25
“Furthermore, local protein synthesis has recently been observed to occur in glial cells (Sakers et al., 2017).”
A cAMP-Related Gene Network in Microglia Is Inversely Regulated by Morphine Tolerance and Withdrawal
Coffey, K. R., Lesiak, A. J., Marx, R. E., Vo, E. K., Garden, G. A., & Neumaier, J. F. (2021) · Biological Psychiatry Global Open Science · doi:10.1016/j.bpsgos.2021.07.011 · Cited by 27
“Microglial ribosomes are expressed in fine processes where they are presumed to translate messenger RNA (mRNA) locally, similar to astrocytes (25).”
BackgroundAyana, R., Zandecki, C., houcke, J. V., Mariën, V., Seuntjens, E., & Arckens, L. (2024) · Aging Cell · doi:10.1111/acel.14251 · Cited by 24
“The detection of SLC1A2B and CX43 transcripts in glial fibers extending into the parenchyma further confirmed the astroglia-like properties (Sakers et al., 2017) of these two RG populations ( Figure 3c,d ).”
BackgroundA transcriptomic atlas of astrocyte heterogeneity across space and time in mouse and marmoset
Schroeder, M. E., McCormack, D. M., Metzner, L. R., Kang, J., Li, K. X., Yu, E., Melamed, L., Levandowski, K. M., Zaniewski, H., Zhang, Q., et al. (2025) · Neuron · doi:10.1016/j.neuron.2025.09.011 · Cited by 30
Kuo, Y.-M., Hsu, P.-C., Hung, C., Hu, Y.-Y., Huang, Y., Gan, Y., Lin, C.-H., Shie, F., Chang, W., Kao, L., et al. (2019) · Molecular Neurobiology · doi:10.1007/s12035-019-01669-8 · Cited by 22
Rurak, G. M., Woodside, B. C., Aguilar‐Valles, A., & Salmaso, N. (2020) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2020.100897 · Cited by 24
Monitoring regional astrocyte diversity by cell type‐specific proteomic labeling in vivo
Prabhakar, P., Pielot, R., Landgraf, P., Wissing, J. B., Bayrhammer, A., van Ham, M., Gundelfinger, E. D., Jänsch, L., Dieterich, D. C., & Müller, A. (2022) · Glia · doi:10.1002/glia.24304 · Cited by 23
Ultrastructural Analysis of Thalamus Damages in a Mouse Model of Osmotic-Induced Demyelination
Bouchat, J., Gilloteaux, J., Suain, V., Vlaender, D. V., Brion, J., & Nicaise, C. (2019) · Neurotoxicity Research · doi:10.1007/s12640-019-00041-x · Cited by 23
Diacylglycerol lipase alpha in astrocytes is involved in maternal care and affective behaviors
Schüle, L., Glasmacher, S., Gertsch, J., Roggan, M. D., Transfeld, J. L., Bîndilă, L., Lutz, B., Kolbe, C., Bilkei‐Gorzó, A., Zimmer, A., et al. (2020) · Glia · doi:10.1002/glia.23903 · Cited by 25
“…astrocytes was low, only about 10% of that of neurons. mRNA signals were often observed outside the cell body, indicating that DAGLa may belong to proteins that are locally translated in distal processes, as it has been shown for other astrocytic proteins (Boulay et al., 2017; Sakers et al., 2017).”
BackgroundPRDM16-DT is a novel lncRNA that regulates astrocyte function in Alzheimer’s disease
Schröder, S., Fuchs, U., Gisa, V., Peña, T., Krüger, D. M., Hempel, N., Burkhardt, S., Salinas, G., Schütz, A., Delalle, I., et al. (2024) · Acta Neuropathologica · doi:10.1007/s00401-024-02787-x · Cited by 27
“…specific conditions, astrocytes express genes typically associated with neurons such as ion channels and genes associated with synaptic function including neurotransmitter receptors, cell adhesion molecules, and other neuromodulatory genes, thus contributing to synaptic modulation [28, 56, 59, 61].”
BackgroundA viral toolkit for recording transcription factor–DNA interactions in live mouse tissues
Cammack, A. J., Moudgil, A., Chen, J., Vasek, M. J., Shabsovich, M., McCullough, K. B., Yen, A., Lagunas, T., Maloney, S. E., He, J., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1918241117 · Cited by 20
“As a positive control, we also cloned the canonical GFAP promoter (pGFAP) (45) into the same location upstream of hsp68::dsRed (Fig.”
BackgroundMethodSelf-citationNG2 Glia: Novel Roles beyond Re-/Myelination
Parolisi, R., & Boda, E. (2018) · Neuroglia · doi:10.3390/neuroglia1010011 · Cited by 23
“Moreover, since these cells establish contacts with neurons and other cell types at specific sites along processes and somata, and local mRNA localization and translation may be instrumental for their crosstalk (as recently shown for astrocytes, [210]), we envisage that novel approaches of spatial transcriptomics may…”
BackgroundRǎdulescu, A., Todd, G. C., Williams, C., Bennink, B., Lemus, A. A., Chesbro, H. E., Bourgeois, J. R., Kopec, A. M., Zuloaga, D. G., & Scimemi, A. (2022) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1009845 · Cited by 24
Makarava, N., Mychko, O., Molesworth, K., Chang, J. C., Henry, R. J., Tsymbalyuk, N., Gerzanich, V., Simard, J. M., Loane, D. J., & Baskakov, I. V. (2023) · Cells · doi:10.3390/cells12172172 · Cited by 19
“Astrocytes translate mRNAs in their peripheral processes that are adjacent to synapses and enriched with mRNAs associated with biological functions, including regulators of synaptic plasticity, GABA, glutamate metabolism, and lipid synthesis [57].”
BackgroundMultifarious astrocyte–neuron dialog in shaping neural circuit architecture
Ngoc, K. H., Jeon, Y., Ko, J., & Um, J. W. (2024) · Trends in Cell Biology · doi:10.1016/j.tcb.2024.05.002 · Cited by 25
Megat, S., & Price, T. J. (2018) · Neurobiology of Pain · doi:10.1016/j.ynpai.2018.02.001 · Cited by 19
“Until now, TRAP technology was mostly used for a comprehensive profiling of the mRNA translation landscape in the targeted cell type (Doyle et al., 2008; Morel et al., 2017; Ouwenga et al., 2017; Sakers et al., 2017).”
MethodOuwenga, R., Lake, A. M., Aryal, S., Lagunas, T., & Dougherty, J. D. (2018) · eNeuro · doi:10.1523/eneuro.0320-18.2018 · Cited by 21
“…indicating that the GFPRpl10a fusion protein was being incorporated into the large subunit in all fractions, and that these subunits were being recruited to 18S bound mRNA (data not shown), consistent with prior studies using this method on other cells (Ouwenga et al., 2017; Sakers et al., 2017).”
ResultSelf-citationLearning and memory formation in zebrafish: Protein dynamics and molecular tools
Reemst, K., Shahin, H., & Shahar, O. D. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1120984 · Cited by 42
“This is important because both in neurons and glial cells, it has been demonstrated that protein synthesis occurs locally, which likely plays an important role in synaptic plasticity (Sutton and Schuman, 2006; Sakers et al., 2017; Spaulding and Burgess, 2017).”
BackgroundFoster, J. B., Zhao, F., Wang, X., Xu, Z., Lin, K. J., Askwith, C. C., Hodgetts, K. J., & Lin, C. G. (2018) · Neuroscience · doi:10.1016/j.neuroscience.2018.07.028 · Cited by 20
“, 2017), only recently have astrocytes been shown to locally translate proteins in distal processes (Boulay et al., 2017; Sakers et al., 2017).”
BackgroundRNA-binding protein quaking: a multifunctional regulator in tumour progression
Zhu, W., Yang, W., Sun, G., & Huang, J. (2024) · Annals of Medicine · doi:10.1080/07853890.2024.2443046 · Cited by 19
Negro, S., Stazi, M., Marchioretto, M., Tebaldi, T., Rodella, U., Duregotti, E., Gerke, V., Quattrone, A., Montecucco, C., Rigoni, M., et al. (2018) · RNA · doi:10.1261/rna.064816.117 · Cited by 19
“918 RNA, Vol. 24, No. 7 PAPs (peripheral astrocytic processes) (Sakers et al. 2017), we hypothesized that Anxa2 mRNA might be transported at the cell borders to be locally translated.”
BackgroundInfluentialAstrocyte-Synapse Structural Plasticity in Neurodegenerative and Neuropsychiatric Diseases
Badia‐Soteras, A., Mak, A., Blok, T. M., Boers-Escuder, C., van den Oever, M. C., Min, R., Smit, A. B., & Verheijen, M. H. G. (2025) · Biological Psychiatry · doi:10.1016/j.biopsych.2025.04.011 · Cited by 21
Oudart, M., Avila‐Gutierrez, K., Moch, C., Dossi, E., Milior, G., Boulay, A.-C., Gaudey, M., Moulard, J., Lombard, B., Loew, D., et al. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112456 · Cited by 18
Charron, A. J., Ward, S. L., North, B. J., Cerón, S., & Leib, D. A. (2019) · Journal of Virology · doi:10.1128/jvi.02246-18 · Cited by 18
SCD Inhibition Protects from α-Synuclein-Induced Neurotoxicity But Is Toxic to Early Neuron Cultures
Nicholatos, J. W., de Groot, J. C. W., Dhokai, S., Tran, D. T., Hrdlicka, L., Carlile, T. M., Bennion, M., Dalkilic-Liddle, I., Hirst, W. D., & Weihofen, A. (2021) · eNeuro · doi:10.1523/eneuro.0166-21.2021 · Cited by 19
“Astrocytes are also reported to have enhanced local translation of SCD and other fatty acid synthesis genes in their peripheral processes (Sakers et al., 2017).”
BackgroundAdenosine A1 Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain
Hackett, T. A. (2018) · The Anatomical Record · doi:10.1002/ar.23907 · Cited by 17
“In addition, it is well established that the transcripts of perhaps thousands of protein coding genes are localized in the neuropil, where local translation may occur (Steward and Levy, 1982; Tiruchinapalli et al., 2003; Bockers et al., 2004; Poon et al., 2006; Zhong et al., 2006; Lein et al., 2007; Giuditta et al., 2…”
BackgroundThe brain's dark transcriptome: Sequencing RNA in distal compartments of neurons and glia
Ament, S. A., & Poulopoulos, A. (2023) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2023.102725 · Cited by 14
Boulay, A.-C., Mazaré, N., Saubaméa, B., & Cohen‐Salmon, M. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9068-9_8 · Cited by 16
Photonics tools begin to clarify astrocyte calcium transients
Gorzo, K. A., & Gordon, G. R. (2022) · Neurophotonics · doi:10.1117/1.nph.9.2.021907 · Cited by 14
Could an Impairment in Local Translation of mRNAs in Glia be Contributing to Pathogenesis in ALS?
Barton, S. K., Gregory, J. M., Chandran, S., & Turner, B. J. (2019) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2019.00124 · Cited by 18
“Other mRNAs known to be translated locally include Aqp4 (encoding the main water channel protein in the CNS responsible for maintaining water homeostasis), Aldh1a1 (encoding an enzyme that depicts mature astrocytes; Boulay et al., 2017), Slc1a2, Slc1a3 and GluI (encoding enzymes important for glutamate metabolism), Fa…”
BackgroundInfluential
Metadata
- Authors
- Sakers, K., Lake, A. M., Khazanchi, R., Ouwenga, R., Vasek, M. J., Dani, A., & Dougherty, J. D.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Dougherty, Joseph D.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-04-24
- Volume
- 114
- Issue
- 19
- Pages
- E3830-E3838
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5441704
- OpenAlex
- W2951270532
- Cited by
- 202
- References
- 72
- Citations by year
- 2026: 24 · 2025: 17 · 2024: 20 · 2023: 25 · 2022: 22 · 2021: 27 · 2020: 20 · 2019: 20 · 2018: 19 · 2017: 8
- FWCI
- 2.26
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Children's Discovery Institute (MD-II-2013-269)
- HHS | National Institutes of Health (DA038458-01, MH099798-01)
- WUSTL Interface of Psychology Neuroscience and Genetics Training Grant (5T32 GM081739)
- WUSTL Neurosciences Program Training Grant (T32 GM008151)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- astrocytes, local translation, mRNA transport, synaptic regulation
- MeSH terms
- Animals, Astrocytes, Cell Nucleus, Humans, Memory, Neurons, RNA, Messenger, Synapses, Protein Biosynthesis
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