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Cited by 223

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Open access
Terekhov, Stanislav S., Smirnov, Ivan V., Stepanova, Anastasiya V., Bobik, Tatyana V., Mokrushina, Yuliana A., Ponomarenko, Natalia A., Belogurov, Alexey A., Rubtsova, Maria P., Kartseva, Olga V., Gomzikova, Marina O., Moskovtsev, Alexey A., Bukatin, Anton S., Dubina, Michael V., Kostryukova, Elena S., Babenko, Vladislav V., Vakhitova, Maria T., Manolov, Alexander I., Malakhova, Maja V., Kornienko, Maria A., Tyakht, Alexander V., Vanyushkina, Anna A., Ilina, Elena N., Masson, Patrick, Gabibov, Alexander G., Altman, Sidney·2017·Proceedings of the National Academy of Sciences

Abstract

Significance: Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. The MDE–FACS platform we developed enabled highly sensitive single-cell selection of predesigned activity and exploration of pairwise interactions between target and effector cells without interference from other microbiota species.

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Metadata

Authors
Terekhov, S. S., Smirnov, I. V., Stepanova, A. V., Bobik, T. V., Mokrushina, Y. A., Ponomarenko, N. A., Belogurov, A. A., Rubtsova, M. P., Kartseva, O. V., Gomzikova, M. O., Moskovtsev, A. A., Bukatin, A. S., Dubina, M. V., Kostryukova, E. S., Babenko, V. V., Vakhitova, M. T., Manolov, A. I., Malakhova, M. V., Kornienko, M. A., Tyakht, A. V., Vanyushkina, A. A., Ilina, E. N., Masson, P., Gabibov, A. G., & Altman, S.
Venue
Proceedings of the National Academy of Sciences
Published
2017-02-15
Volume
114
Issue
10
Pages
2550-2555
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2588524741
Cited by
223
References
1
FWCI
10.48
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity. https://app.cochat.ai/papers/10.1073/pnas.1621226114
The paper
Terekhov, S. S., Smirnov, I. V., Stepanova, A. V., Bobik, T. V., Mokrushina, Y. A., Ponomarenko, N. A., Belogurov, A. A., Rubtsova, M. P., Kartseva, O. V., Gomzikova, M. O., Moskovtsev, A. A., Bukatin, A. S., Dubina, M. V., Kostryukova, E. S., Babenko, V. V., Vakhitova, M. T., Manolov, A. I., Malakhova, M. V., Kornienko, M. A., . . . Altman, S. (2017). Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1621226114

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.