The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Daisy-chain gene drives for the alteration of local populations
Open accessAbstract
If they are able to spread in wild populations, CRISPR-based gene-drive elements would provide new ways to address ecological problems by altering the traits of wild organisms, but the potential for uncontrolled spread tremendously complicates ethical development and use. Here, we detail a self-exhausting form of CRISPR-based drive system comprising genetic elements arranged in a daisy chain such that each drives the next. “Daisy-drive” systems can locally duplicate any effect achievable by using an equivalent self-propagating drive system, but their capacity to spread is limited by the successive loss of nondriving elements from one end of the chain. Releasing daisy-drive organisms constituting a small fraction of the local wild population can drive a useful genetic element nearly to local fixation for a wide range of fitness parameters without self-propagating spread. We additionally report numerous highly active guide RNA sequences sharing minimal homology that may enable evolutionarily stable daisy drive as well as self-propagating CRISPR-based gene drive. Especially when combined with threshold dependence, daisy drives could simplify decision-making and promote ethical use by enabling local communities to decide whether, when, and how to alter local ecosystems.
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Citations
Cites
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature · doi:10.1038/nature16526 · Cited by 2,673
Rationally engineered Cas9 nucleases with improved specificity
Slaymaker, I. M., Gao, L., Zetsche, B., Scott, D. A., Yan, W. X., & Zhang, F. (2016) · Science · doi:10.1126/science.aad5227 · Cited by 2,414
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila
Port, F., Chen, H.-M., Lee, T., & Bullock, S. L. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405500111 · Cited by 1,046
Cited by
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
Port, F., & Bullock, S. L. (2016) · Nature Methods · doi:10.1038/nmeth.3972 · Cited by 437
Evolution of Resistance Against CRISPR/Cas9 Gene Drive
Unckless, R. L., Clark, A. G., & Messer, P. W. (2017) · Genetics · doi:10.1534/genetics.116.197285 · Cited by 294
Metadata
- Authors
- Noble, C., Min, J., Olejarz, J., Buchthal, J., Chavez, A., Smidler, A. L., DeBenedictis, E. A., Church, G. M., Nowak, M. A., & Esvelt, K. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2019-04-02
- Volume
- 116
- Issue
- 17
- Pages
- 8275-8282
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2952260018
- Cited by
- 233
- References
- 3
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Noble, C., Min, J., Olejarz, J., Buchthal, J., Chavez, A., Smidler, A. L., DeBenedictis, E. A., Church, G. M., Nowak, M. A., & Esvelt, K. M. (2019). Daisy-chain gene drives for the alteration of local populations. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1716358116
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