Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

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The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations

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Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

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A genome-wide resource for the analysis of protein localisation in Drosophila

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RNAi screening comes of age: improved techniques and complementary approaches

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Safeguarding CRISPR-Cas9 gene drives in yeast

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Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

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Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations

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Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans

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Safeguarding gene drive experiments in the laboratory

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CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis

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Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila

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The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila

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Genome organization controls transcriptional dynamics during development

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Daisy-chain gene drives for the alteration of local populations

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A CRISPR view of developmentPreprint

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CRISPR‐Cas9 Genome Editing in Drosophila

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Identification of Happyhour/MAP4K as Alternative Hpo/Mst-like Kinases in the Hippo Kinase Cascade

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Interorgan Communication Pathways in Physiology: Focus on Drosophila

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Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis

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Energy Homeostasis Control in <i>Drosophila</i> Adipokinetic Hormone Mutants

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Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators

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Drosophila Lgr3 Couples Organ Growth with Maturation and Ensures Developmental Stability

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Panoramix enforces piRNA-dependent cotranscriptional silencing

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Genome editing retraces the evolution of toxin resistance in the monarch butterfly

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Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods

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Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing

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A sleep-inducing gene, nemuri , links sleep and immune function in Drosophila

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Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi

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Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti

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Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges

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More than one rabbit out of the hat: Radiation, transgenic and symbiont-based approaches for sustainable management of mosquito and tsetse fly populations

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Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

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Functional validation of cadherin as a receptor of Bt toxin Cry1Ac in Helicoverpa armigera utilizing the CRISPR/Cas9 system

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Loss of Parkinson’s disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c

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Efficient CRISPR-mediated gene targeting and transgene replacement in the beetleTribolium castaneum

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A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard

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An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

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Patronin/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis

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Overcoming evolved resistance to population-suppressing homing-based gene drives

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Exploring the potential of genome editing CRISPR-Cas9 technology

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A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila

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Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive

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Advances in Engineering the Fly Genome with the CRISPR-Cas System

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Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

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CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

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Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo

Barckmann, B., Pierson, S., Dufourt, J., Papin, C., Armenise, C., Port, F., Grentzinger, T., Chambeyron, S., Baronian, G., Desvignes, J., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.07.030 · Cited by 142 · see citing works

Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

Task, D., Lin, C.-C., Vulpe, A., Afify, A., Ballou, S., Brbic, M., Schlegel, P., Raji, J., Jefferis, G. S., Li, H., et al. (2022) · eLife · doi:10.7554/elife.72599 · Cited by 141 · see citing works

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

Song, L., Ouedraogo, J., Kolbusz, M. A., Nguyen, T. T. M., & Tsang, A. (2018) · PLoS ONE · doi:10.1371/journal.pone.0202868 · Cited by 139 · see citing works

Glypicans shield the Wnt lipid moiety to enable signalling at a distance

McGough, I. J., Vecchia, L., Bishop, B., Malinauskas, T., Beckett, K., Joshi, D., O’Reilly, N., Siebold, C., Jones, E. Y., & Vincent, J. (2020) · Nature · doi:10.1038/s41586-020-2498-z · Cited by 139 · see citing works

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

Thomas, H. R., Percival, S. M., Yoder, B. K., & Parant, J. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0114632 · Cited by 137 · see citing works

An Insulin-Sensitive Circular RNA that Regulates Lifespan in Drosophila

Weigelt, C. M., Sehgal, R., Tain, L. S., Cheng, J., Eßer, J., Pahl, A., Dieterich, C., Grönke, S., & Partridge, L. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.06.011 · Cited by 134 · see citing works

In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila

Lin, S., Ewen‐Campen, B., Ni, X., Housden, B. E., & Perrimon, N. (2015) · Genetics · doi:10.1534/genetics.115.181065 · Cited by 136 · see citing works

Large-Scale Transgenic Drosophila Resource Collections for Loss- and Gain-of-Function Studies

Zirin, J., Hu, Y.-H., Liu, L.-P., Yang-Zhou, D., Colbeth, R., Yan, D., Ewen-Campen, B., Tao, R., Vogt, E., VanNest, S., et al. (2019) · Genetics · doi:10.1534/genetics.119.302964 · Cited by 123 · see citing works

RNA Interference (RNAi) Screening in <i>Drosophila</i>

Heigwer, F., Port, F., & Boutros, M. (2018) · Genetics · doi:10.1534/genetics.117.300077 · Cited by 125 · see citing works

Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs

Champer, S. E., Oh, S. Y., Liu, C., Wen, Z., Clark, A. G., Messer, P. W., & Champer, J. (2020) · Science Advances · doi:10.1126/sciadv.aaz0525 · Cited by 135 · see citing works

A toxin-antidote CRISPR gene drive system for regional population modification

Champer, J., Lee, E., Yang, E., Liu, C., Clark, A. G., & Messer, P. W. (2020) · Nature Communications · doi:10.1038/s41467-020-14960-3 · Cited by 134 · see citing works

Connectomics Analysis Reveals First-, Second-, and Third-Order Thermosensory and Hygrosensory Neurons in the Adult Drosophila Brain

Marin, E. C., Büld, L., Theiss, M., Sarkissian, T., Roberts, R. J. V., Turnbull, R., Tamimi, I. F. M., Pleijzier, M. W., Laursen, W. J., Drummond, N., et al. (2020) · Current Biology · doi:10.1016/j.cub.2020.06.028 · Cited by 121 · see citing works

Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System

Armstrong, G. A. B., Liao, M., You, Z., Lissouba, A., Chen, B. E., & Drapeau, P. (2016) · PLoS ONE · doi:10.1371/journal.pone.0150188 · Cited by 131 · see citing works

A CRISPR homing gene drive targeting a haplolethal gene removes resistance alleles and successfully spreads through a cage population

Champer, J., Yang, E., Lee, E., Liu, J., Clark, A. G., & Messer, P. W. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2004373117 · Cited by 130 · see citing works

RNP-Granule Assembly via Ataxin-2 Disordered Domains Is Required for Long-Term Memory and Neurodegeneration

Bakthavachalu, B., Huelsmeier, J., Sudhakaran, I. P., Hillebrand, J., Singh, A., Petrauskas, A., Thiagarajan, D., Sankaranarayanan, M., Mizoue, L. S., Anderson, E. N., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.04.032 · Cited by 121 · see citing works

Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems

Khambhati, K., Bhattacharjee, G., Gohil, N., Braddick, D., Kulkarni, V. V., & Singh, V. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00248 · Cited by 127 · see citing works

Drosophila melanogaster Oogenesis: An Overview

McLaughlin, J. M., & Bratu, D. P. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2851-4_1 · Cited by 125 · see citing works

A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production

ElMaghraby, M. F., Andersen, P. R., Pühringer, F., Hohmann, U., Meixner, K., Lendl, T., Tirián, L., & Brennecke, J. (2019) · Cell · doi:10.1016/j.cell.2019.07.007 · Cited by 116 · see citing works

A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila

Zhang, X., Koolhaas, W. H., & Schnorrer, F. (2014) · G3 Genes Genomes Genetics · doi:10.1534/g3.114.013979 · Cited by 122 · see citing works

A mutation in the PSST homologue of complex I (NADH:ubiquinone oxidoreductase) from Tetranychus urticae is associated with resistance to METI acaricides

Bajda, S., Dermauw, W., Panteleri, R., Sugimoto, N., Douris, V., Tirry, L., Osakabe, M., VONTAS, J. G., & Leeuwen, T. V. (2016) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2016.11.010 · Cited by 119 · see citing works

An alternative mode of epithelial polarity in the Drosophila midgut

Chen, J., Sayadian, A.-C., Lowe, N., Lovegrove, H. E., & St Johnston, D. (2018) · PLOS Biology · doi:10.1371/journal.pbio.3000041 · Cited by 118 · see citing works

A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity

King, A. N., Barber, A. F., Smith, A. E., Dreyer, A. P., Sitaraman, D., Nitabach, M. N., Cavanaugh, D. J., & Sehgal, A. (2017) · Current Biology · doi:10.1016/j.cub.2017.05.089 · Cited by 117 · see citing works

Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila

Gokcezade, J., Sienski, G., & Duchek, P. (2014) · G3 Genes Genomes Genetics · doi:10.1534/g3.114.014126 · Cited by 116 · see citing works

A CRISPR/Cas9 mediated point mutation in the alpha 6 subunit of the nicotinic acetylcholine receptor confers resistance to spinosad in Drosophila melanogaster

Zimmer, C. T., Garrood, W. T., Puinean, A. M., Manuela, E.-Z., Williamson, M. S., Davies, T. G. E., & Bass, C. (2016) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2016.04.007 · Cited by 114 · see citing works

Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs

Oberhofer, G., Ivy, T., & Hay, B. A. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1805278115 · Cited by 111 · see citing works

Molecular safeguarding of CRISPR gene drive experiments

Champer, J., Chung, J., Lee, Y. L., Liu, C., Yang, E., Wen, Z., Clark, A. G., & Messer, P. W. (2019) · eLife · doi:10.7554/elife.41439 · Cited by 111 · see citing works

Ankle2, a Target of Zika Virus, Controls Asymmetric Cell Division of Neuroblasts and Uncovers a Novel Microcephaly Pathway

Link, N., Chung, H.-L., Jolly, A., Withers, M. A., Tepe, B., Arenkiel, B. R., Shah, P. S., Krogan, N. J., Aydın, H., Geçkinli, B. B., et al. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.10.009 · Cited by 108 · see citing works

A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila

Huynh, N., Depner, N., Larson, R. T., & King‐Jones, K. (2020) · Genome biology · doi:10.1186/s13059-020-02193-y · Cited by 110 · see citing works

Neural Evolution of Context-Dependent Fly Song

Ding, Y., Lillvis, J. L., Cande, J., Berman, G. J., Arthur, B. J., Long, X., Xu, M., Dickson, B. J., & Stern, D. L. (2019) · Current Biology · doi:10.1016/j.cub.2019.02.019 · Cited by 109 · see citing works

Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster

Lin, C., & Potter, C. J. (2016) · Genetics · doi:10.1534/genetics.116.191783 · Cited by 108 · see citing works

Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons.

Task, D., Lin, C., Vulpe, A., Afify, A., Ballou, S., Brbić, M., Schlegel, P., Raji, J. I., Jefferis, G. S. X. E., Li, H., et al. (2021) · Apollo (University of Cambridge) · doi:10.17863/cam.83721 · Cited by 108 · see citing works

Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution

Gilles, A. F., & Averof, M. (2014) · EvoDevo · doi:10.1186/2041-9139-5-43 · Cited by 110 · see citing works

Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Meltzer, H., Marom, E., Alyagor, I., Mayseless, O., Berkun, V., Segal-Gilboa, N., Unger, T., Luginbuhl, D. J., & Schuldiner, O. (2019) · Nature Communications · doi:10.1038/s41467-019-10140-0 · Cited by 97 · see citing works

CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions

Kaushal, A., Mohana, G., Dorier, J., Özdemir, İ., Omer, A., Cousin, P., Semenova, A. I., Taschner, M., Dergai, O., Marzetta, F., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-21366-2 · Cited by 99 · see citing works

Environmentally sustainable pest control options forDrosophila suzukii

Schetelig, M. F., Lee, K., Otto, S., Talmann, L., Stökl, J., Degenkolb, T., Vilcinskas, A., & Halitschke, R. (2017) · Journal of Applied Entomology · doi:10.1111/jen.12469 · Cited by 104 · see citing works

CRISPR/Cas9-mediated mutagenesis of the white and Sex lethal loci in the invasive pest, Drosophila suzukii

Li, F., & Scott, M. J. (2015) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2015.12.081 · Cited by 103 · see citing works

The dawn of active genetics

Gantz, V. M., & Bier, E. (2015) · BioEssays · doi:10.1002/bies.201500102 · Cited by 103 · see citing works

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

Zhang, J., Li, X., Neises, A., Chen, W., Hu, L., Ji, G., Yu, J.-Y., Xu, J., Yuan, W., Cheng, T., et al. (2016) · Scientific Reports · doi:10.1038/srep28566 · Cited by 104 · see citing works

Doublesex Regulates the Connectivity of a Neural Circuit Controlling Drosophila Male Courtship Song

Shirangi, T. R., Wong, A. M., Truman, J. W., & Stern, D. L. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.05.012 · Cited by 101 · see citing works

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans

Au, V., Li-Leger, E., Raymant, G., Flibotte, S., Chen, G., Martin, K., Fernando, L., Doell, C., Rosell, F. I., Wang, S., et al. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200778 · Cited by 95 · see citing works

Xio is a component of the Drosophila sex determination pathway and RNA N 6 -methyladenosine methyltransferase complex

Guo, J., Tang, H.-W., Li, J., Perrimon, N., & Yan, D. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1720945115 · Cited by 100 · see citing works

Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9

van Tienen, L. M., Mieszczanek, J., Fiedler, M., Rutherford, T. J., & Bienz, M. (2017) · eLife · doi:10.7554/elife.20882 · Cited by 98 · see citing works

Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila

Evantal, N., Anduaga, A. M., Bartok, O., Patop, I. L., Weiss, R., & Kadener, S. (2019) · eLife · doi:10.7554/elife.44642 · Cited by 98 · see citing works

The gut hormone Allatostatin C/Somatostatin regulates food intake and metabolic homeostasis under nutrient stress

Kubrak, O. I., Koyama, T., Ahrentløv, N., Jensen, L., Malita, A., Naseem, M. T., Lassen, M., Nagy, S., Texada, M. J., Halberg, K. A., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-28268-x · Cited by 97 · see citing works

Oxidative stress induces stem cell proliferation via TRPA1/RyR-mediated Ca2+ signaling in the Drosophila midgut

Xu, C., Luo, J., He, L., Montell, C., & Perrimon, N. (2017) · eLife · doi:10.7554/elife.22441 · Cited by 96 · see citing works

Investigation of the contribution of RyR target-site mutations in diamide resistance by CRISPR/Cas9 genome modification in Drosophila

Douris, V., Papapostolou, K. M., Ilias, A., Roditakis, E. N., Kounadi, S., Riga, M., Nauen, R., & VONTAS, J. G. (2017) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2017.06.013 · Cited by 95 · see citing works

Apical stress fibers enable a scaling between cell mechanical response and area in epithelial tissue

López-Gay, J. M., Nunley, H., Spencer, M., di Pietro, F., Guirao, B., Bosveld, F., Markova, O., Gaugué, I., Pelletier, S., Lubensky, D. K., et al. (2020) · Science · doi:10.1126/science.abb2169 · Cited by 95 · see citing works

Precise genome engineering in Drosophila using prime editing

Bosch, J. A., Birchak, G., & Perrimon, N. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2021996118 · Cited by 94 · see citing works

C9orf72 -derived arginine-containing dipeptide repeats associate with axonal transport machinery and impede microtubule-based motility

Fumagalli, L., Young, F. L., Boeynaems, S., Decker, M. D., Mehta, A. R., Swijsen, A., Fazal, R., Guo, W., Moisse, M., Beckers, J., et al. (2021) · Science Advances · doi:10.1126/sciadv.abg3013 · Cited by 89 · see citing works

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

Gratz, S. J., Cummings, A. M., Nguyen, J. N., Hamm, D. C., Donohue, L. K., Harrison, M. M., Wildonger, J., & O’Connor-Giles, K. M. (2013) · Genetics · doi:10.1534/genetics.113.152710 · Cited by 959 · see citing works

CRISPR‐Cas9 Genome Editing in Drosophila

Gratz, S. J., Rubinstein, C. D., Harrison, M. M., Wildonger, J., & O’Connor-Giles, K. M. (2015) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb3102s111 · Cited by 214 · see citing works

CRISPR-/Cas9-Mediated Precise and Efficient Genome Editing in Drosophila

Nyberg, K. G., & Carthew, R. W. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2541-5_6 · Cited by 7 · see citing works

Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila

Koreman, G. T., Xu, Y., Hu, Q., Zhang, Z., Allen, S. E., Wolfner, M. F., Wang, B., & Han, C. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014255118 · Cited by 29 · see citing works

Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila

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