Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803

Aizouq, Mohammed; Peisker, Helga; Gutbrod, Katharina; Melzer, Michael; Hölzl, Georg; Dörmann, Peter

doi:10.1073/pnas.1915930117

Cited by 49Lipid metabolism and biosynthesisAlgal biology and biofuel productionMicrobial Metabolic Engineering and Bioproduction

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Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803

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Aizouq, Mohammed, Peisker, Helga, Gutbrod, Katharina, Melzer, Michael, Hölzl, Georg, Dörmann, Peter·2020·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1915930117PMC PMC7084069

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Abstract

Significance: Cyanobacteria harbor a photosynthetic apparatus related to plant chloroplasts. The lipid compositions of the thylakoids that harbor the photosynthetic complexes in cyanobacteria and chloroplasts are highly similar. Chloroplasts contain triacylglycerol (storage oil) and wax esters; the latter are composed of phytol derived from chlorophyll and fatty acids (phytyl esters). However, the existence of these lipids in cyanobacteria in general remained unclear. Here we show that the cyanobacterium Synechocystis contains triacylglycerol and phytyl esters. A mutant, Δslr2103, was generated, which lacked these two lipids but showed no obvious growth defect. The slr2103 gene encodes a diacylglycerol acyltransferase different from known enzymes of triacylglycerol synthesis in bacteria. This pathway can be employed to produce oil for biotechnological applications in cyanobacteria.

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TAG-like molecules (lipid X) have been detected in picomol concentrations in the model Synechocystis.
“TAG-like molecules (lipid X) have been detected in picomol concentrations in the model Synechocystis [68,69].”
From Spirulina/Arthrospira/Limnospira—Three Names of the Single Organism (2024) · cites this paper as [68]
The Results report detecting TAG species in Synechocystis, supporting the detection claim. They quantify total TAG in wild-type cells at approximately 5 nmol per OD750 unit, not in picomoles; the approximately 0.3 nmol value is for a mutant. The paper also identifies the molecules as bona fide TAG rather than merely TAG-like. (LLM, conf=0.88)
Experimental — accuracy not yet measured
TAG content in cyanobacteria is generally low.
“While a recent study challenges the notion that natural cyanobacteria lack neutral lipid TAG, it remains a fact that TAG content in cyanobacteria is generally low [37, 38].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [38]

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Authors: Aizouq, M., Peisker, H., Gutbrod, K., Melzer, M., Hölzl, G., & Dörmann, P.
Affiliations: University of Bonn (DE)
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) (DE)
Corresponding author: Dörmann, Peter
ORCID: Peisker, Helga
Gutbrod, Katharina
Melzer, Michael
Hölzl, Georg
Dörmann, Peter
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2020-03-02
Volume: 117
Issue: 11
Pages: 6216-6222
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1915930117
PMC: PMC7084069
OpenAlex: W3009593311
Cited by: 49
References: 39
Citations by year: 2026: 5 · 2025: 11 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 8 · 2020: 6
FWCI: 2.89
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.pnas.org/site/aboutpnas/licenses.xhtml (vor, 184 d)
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Rheinische Friedrich-Wilhelms-Universität Bonn (Az 3.322-00050381)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: triglyceride synthesis, wax esters, Synechocystis sp. PCC 6803, cyanobacteria, diacylglycerol acyltransferase, thylakoid lipids, phytol, storage lipids
MeSH terms: Bacterial Proteins, Esters, Phytol, Triglycerides, Waxes, Synechocystis, Diacylglycerol O-Acyltransferase, Gene Knockout Techniques

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Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803

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