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Cited by 24Lipid metabolism and biosynthesisAlgal biology and biofuel productionMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Quantitative and Qualitative Analyses of Triacylglycerol Production in the Wild-Type Cyanobacterium Synechocystis sp. PCC 6803 and the Strain Expressing AtfA from Acinetobacter baylyi ADP1

Tanaka, Motoki, Ishikawa, Toshiki, Tamura, So, Saito, Yujiro, Kawai-Yamada, Maki, Hihara, Yukako·2020·Plant and Cell Physiology
DOI 10.1093/pcp/pcaa069
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Abstract

Although cyanobacteria do not possess wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT), the bacterial enzyme for triacylglycerol (TAG) production, there have been several studies reporting the accumulation of TAG-like compounds in cyanobacteria. In this study, we aimed to evaluate TAG productivity of the ΔrecJ::atfA strain of Synechocystis sp. PCC 6803 generated by inserting atfA encoding WS/DGAT from Acinetobacter baylyi ADP1 into recJ (sll1354), together with the wild type (WT) and the gene-disrupted strain of slr2103 having homology with eukaryotic DGAT2 gene family (Δ2103). Thin-layer chromatography (TLC) of neutral lipids or isolation of the neutral lipid-enriched fraction followed by gas chromatography or liquid chromatography-tandem mass spectrometry was employed for analyses. The ΔrecJ::atfA strain accumulated 0.508 nmol ml-1OD730-1 of TAG after a week of incubation at 100 μmol photons m-2 s-1. The saturated fatty acids C16:0 and C18:0 accounted for about 50% and 20% of the TAG fatty acids, respectively, suggesting that de novo-synthesized fatty acids were preferentially incorporated into TAG molecules. When the neutral lipid profile of the lipid extracts was examined by TLC, a spot located in a slightly lower position compared with the TAG standard was detected in WT but not in the Δ2103 strain. TAG accumulation levels of both strains was only 0.01-0.03 nmol ml-1OD730-1, but the fatty acid composition was substantially different from that of the background. These results suggest that trace amounts of TAG can be produced in Synechocystis cells by enzymes other than Slr2103, and major constituents of the TAG-like spot are unknown lipid species produced by Slr2103.

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Crossref · 10.1093/pcp/pcaa069

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  • TAG-like molecules (lipid X) have been detected in picomol concentrations in the model Synechocystis.

    “TAG-like molecules (lipid X) have been detected in picomol concentrations in the model Synechocystis [68,69].”

    From Spirulina/Arthrospira/Limnospira—Three Names of the Single Organism (2024) · cites this paper as [69]

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  1. Microbiology, 989

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Metadata

Authors
Tanaka, M., Ishikawa, T., Tamura, S., Saito, Y., Kawai-Yamada, M., & Hihara, Y.
Affiliations
  • Saitama University (JP)
  • Department of Environmental Science and Technology, Graduate School of Science and Engineering, Saitama University, Saitama, 338-8570 Japan
Corresponding author
Hihara, Yukako
ORCID
Venue
Plant and Cell Physiology
Publisher
Oxford University Press (OUP)
Published
2020-05-20
Volume
61
Issue
9
Pages
1537-1547
ISSN
  • 0032-0781 (ISSN-L)
  • 1471-9053
DOI
10.1093/pcp/pcaa069
OpenAlex
W3027087034
Cited by
24
References
35
Citations by year
2026: 2 · 2025: 6 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 1
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Plant and Cell Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • The Japan Science and Technology Agency (JST) PRESTO
  • CREST
  • Japan Society for the Promotion of Science (JSPS) KAKENHI (15K07096, 19K06717)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
triglyceride synthesis, Synechocystis sp. PCC 6803, Acinetobacter baylyi ADP1, AtfA, diacylglycerol acyltransferase, DGAT2, RecJ, fatty acid composition, neutral lipids, heterologous expression, de novo lipogenesis
MeSH terms
Acinetobacter, Chromatography, Gas, Chromatography, Thin Layer, Lipids, Gas Chromatography-Mass Spectrometry, Triglycerides, Organisms, Genetically Modified, Synechocystis, Diacylglycerol O-Acyltransferase

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