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Cited by 17Contact Dermatitis and AllergiesDermatology and Skin DiseasesT-cell and B-cell Immunology

Selective AhR knockout in langerin-expressing cells abates Langerhans cells and polarizes Th2/Tr1 in epicutaneous protein sensitization

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Hong, Chien-Hui, Lin, Shang-Hung, Clausen, Björn E., Lee, Chih-Hung·2020·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1917479117PMC PMC7293700
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Abstract

Significance: AhR, which is expressed in many epithelial, mucosal, and immune cells, represents a vital cellular sensor of environmental factors. Immune regulation by AhR can be cell, tissue, and ligand specific. Langerhans cells (LC), professional antigen presenting cells in the epidermis, govern the immunological barrier against environmental threats. How the AhR in LC regulates epicutaneous immune responses remains unclear. Experimenting with Langerin-specific AhR −/− mice, we discovered that AhR-deficient epidermal LC were reduced in number and, following epicutaneous protein (Ova) immunization, promoted T helper type-2 (Th2) and T regulatory type-1 (Tr1) responses, leading to increased Ova-specific IgE in the blood. Thus, AhR represents a critical sensor regulating LC activation and LC-mediated T cell polarization.

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  • AHR has cell-specific roles in lymphocytes, intestinal epithelial cells, keratinocytes, Langerhans cells, and hematopoietic stem cells.

    “Subsequent examination of this mouse crossed to various Cre models has unveiled a cell specific role for AHR in lymphocytes, intestinal epithelial cells, keratinocytes, Langerhans cells, and hematopoietic stem cells to name a few (Bennett et al., 2018; Haas et al., 2016; Hong et al., 2020; Li et al., 2018; Metidji et al., 2018)”

    From Rodent Genetic Models of Ah Receptor Signaling (2021) · cites this paper as Hong et al., 2020

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Cited by

Sorted by citations

  • Rodent Genetic Models of Ah Receptor Signaling

    Wilson, R. H., & Bradfield, C. A. (2021) · Drug Metabolism Reviews · doi:10.1080/03602532.2021.1955916 · Cited by 16 · see citing works

    “…intestinal epithelial cells, keratinocytes, Langerhans cells, and hematopoietic stem cells, to name a few (Haas et al. 2016; Li et al. 2017; Bennett et al. 2018; Metidji et al. 2018; Hong et al. 2020) This mouse has been a vital tool in untangling the complicated cell-specific role of AHR.”
    Method

Authors
Hong, C.-H., Lin, S.-H., Clausen, B. E., & Lee, C.-H.
Affiliations
  • Kaohsiung Veterans General Hospital (TW)
  • Chang Gung University (TW)
  • Johannes Gutenberg University Mainz (DE)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2020-05-27
Volume
117
Issue
23
Pages
12980-12990
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1917479117
PMC
PMC7293700
OpenAlex
W3030163031
Cited by
17
References
3
Citations by year
2025: 5 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 1
FWCI
2.01
Type
journal article
Language
en
Open access
Licence
www.pnas.org/site/aboutpnas/licenses.xhtml (vor, 184 d)
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Science and Technology, Taiwan (107-2314-B-182A-082-MY3, 108-2314-B-010-045-MY3)
  • Chang Gung Medical Foundation (CMRPG8K0291, CMRPG8I0361)
  • Kaohsiung Veterans General Hospital (VGHKS108-139)
Topics
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
aryl hydrocarbon receptor, Langerhans cells, Langerin, epicutaneous sensitization, ovalbumin, Th2 polarization, regulatory T cells, IgE, T cell polarization
MeSH terms
Administration, Cutaneous, Animals, Antigens, Surface, Epidermis, Immunoglobulin E, Langerhans Cells, Mice, Transgenic, Ovalbumin, Receptors, Aryl Hydrocarbon, Th2 Cells, Lectins, C-Type, Mannose-Binding Lectins, T-Lymphocytes, Regulatory, Mice, Basic Helix-Loop-Helix Transcription Factors, Basic Helix-Loop-Helix Proteins, Gene Knockout Techniques

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