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Cited by 112Neuroscience and Neuropharmacology ResearchReceptor Mechanisms and SignalingAdvanced Fluorescence Microscopy Techniques

Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses

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Goncalves, Julia, Bartol, Tomas M., Camus, Côme, Levet, Florian, Menegolla, Ana Paula, Sejnowski, Terrence J., Sibarita, Jean-Baptiste, Vivaudou, Michel +2 more·2020·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1922563117PMC PMC7321977
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Abstract

Significance: Neuron-to-neuron communication is based on synapse activity where presynapses release neurotransmitters, which activate postsynaptic neurotransmitter receptors. The various families of glutamate receptors at the excitatory synapses are responsible of the fast synaptic transmission as well as the regulation of the long-term signaling implicated in information storage and memory. The organization at the nanometer scale of these postsynaptic receptors is a key determinant for synaptic transmission efficiency. Here, we combined dual-color superresolution imaging with electrophysiology and modeling to determine how the various glutamate receptors are co-organized at the nanoscale and to what extent this organization regulates the receptor activation by a single vesicle release.

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Crossref · 10.1073/pnas.1922563117

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  • When the CTD of neuroligin 1 is included, it partitions with AMPAR/TARPs but not with NMDARs, reminiscent of nanocolumns in the PSD.

    “Remarkably, when the CTD of neuroligin 1 is included, it partitions with the AMPAR/TARPs but not with the NMDARs, reminiscent of nanocolumns in the PSD (251–253)”

    From Synaptic memory and CaMKII (2023) · cites this paper as [253]

    The Discussion (page not specified) reports that AMPAR and NMDAR clusters barely colocalize at the nanoscale, supporting the comparison to distinct PSD domains. It does not report that the CTD of neuroligin 1 partitions with AMPAR/TARPs rather than NMDARs. (LLM, conf=0.83)

    Experimental — accuracy not yet measured

  • NMDARs tend to be concentrated in the middle of the PSD with little overlap with AMPARs.

    “NMDARs tend to be concentrated in the middle of the PSD with little overlap with AMPARs (253–255).”

    From Synaptic memory and CaMKII (2023) · cites this paper as [253]

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Research works printed but not linked: 34

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Authors
Goncalves, J., Bartol, T. M., Camus, C., Levet, F., Menegolla, A. P., Sejnowski, T. J., Sibarita, J.-B., Vivaudou, M., Choquet, D., & Hosy, E.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Salk Institute for Biological Studies (US)
  • Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037;
Corresponding author
Hosy, Eric
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2020-06-08
Volume
117
Issue
25
Pages
14503-14511
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1922563117
PMC
PMC7321977
OpenAlex
W3033163957
Cited by
112
References
5
Citations by year
2026: 7 · 2025: 13 · 2024: 21 · 2023: 16 · 2022: 16 · 2021: 35 · 2020: 4
FWCI
8.14
Type
journal article
Language
en
Open access
Licence
www.pnas.org/site/aboutpnas/licenses.xhtml (vor, 183 d)
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • HAL Portal Artxiker (Hindustan Aeronautics Limited (India)) (submittedVersion)
Funders
  • Agence Nationale de la Recherche (ANR-16-CE13-0018, ANR-15-CE11-0029, ANR-11-LABX-0015, ANR-17-EURE-0028)
  • National Institute of Mental Health (R01MH115556)
  • National Institute of General Medical Sciences (P41GM103712)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
AMPA receptors, NMDA receptor, mGluR5, metabotropic glutamate receptors, excitatory synapses, postsynaptic density, receptor coactivation, glutamate release, synaptic transmission, receptor clustering
MeSH terms
Intravital Microscopy, Single Molecule Imaging, Animals, Cells, Cultured, Embryo, Mammalian, Female, Hippocampus, Models, Neurological, Synaptic Transmission, Neurons, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Receptors, AMPA, Patch-Clamp Techniques, Glutamic Acid, Rats, Primary Cell Culture, Receptor, Metabotropic Glutamate 5

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