Discovery of a small protein factor involved in the coordinated degradation of phycobilisomes in cyanobacteria
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Abstract
Significance: During genome analysis, genes encoding small proteins are frequently neglected. Accordingly, small proteins have remained underinvestigated in all domains of life. Based on a previous systematic search for such genes, we present the functional analysis of the 66 amino acids protein NblD in a photosynthetic cyanobacterium. We show that NblD plays a crucial role during the coordinated dismantling of phycobilisome light-harvesting complexes. This disassembly is triggered when the cells become starved for nitrogen, a condition that frequently occurs in nature. Similar to NblA that tags phycobiliproteins for proteolysis, NblD binds to phycocyanin polypeptides but has a different function. The results show that, even in a well-investigated process, crucial new players can be discovered if small proteins are taken into consideration.
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- Authors
- Krauspe, V., Fahrner, M., Spät, P., Steglich, C., Frankenberg-Dinkel, N., Maček, B., Schilling, O., & Hess, W. R.
- Affiliations
- University of Freiburg (DE)
- University of Tübingen (DE)
- Department of Microbiology, Faculty of Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany
- Corresponding author
- Hess, Wolfgang R.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2021-01-28
- Volume
- 118
- Issue
- 5
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC7865187
- OpenAlex
- W3123609947
- Cited by
- 54
- References
- 4
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 8 · 2023: 10 · 2022: 7 · 2021: 6 · 2020: 1
- FWCI
- 2.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (HE2544/12-1, FOR2816, 322977937/GRK2344, SCHI 871/11-1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Keywords
- small proteins, phycobilisome degradation, phycocyanin, cyanobacteria, nitrogen starvation, protein degradation, photosynthesis
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Models, Biological, Mutation, Nitrogen, Phenotype, Photosynthesis, Phylogeny, Protein Binding, RNA, Messenger, Gene Expression Regulation, Bacterial, Conserved Sequence, Phycobilisomes, Synechocystis, Transcriptome
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