The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy
Open accessAbstract
Existing methods for studying actin filament dynamics have allowed analysis only of bulk samples or individual filaments after treatment with the drug phalloidin, which perturbs filament dynamics. Total internal reflection fluorescence microscopy with rhodamine-labeled actin allowed us to observe polymerization in real time, without phalloidin. Direct measurements of filament growth confirmed the rate constants measured by electron microscopy and established that rhodamine actin is a kinetically inactive tracer for imaging. In the presence of activated Arp2/3 complex, growing actin filaments form branches at random sites along their sides, rather than preferentially from their barbed ends.
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Cites
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Molecular Mechanisms Controlling Actin Filament Dynamics in Nonmuscle Cells
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Self-citationRate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments.
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Self-citationActin microfilament dynamics in locomoting cells
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Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
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Self-citationBlanchoin, L., Amann, K. J., Higgs, H. N., Marchand, J.-B., Kaiser, D. A., & Pollard, T. D. (2000) · Nature · doi:10.1038/35010008 · Cited by 586
Self-citationCrystal Structure of Arp2/3 Complex
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Cited by
Actin Dynamics, Architecture, and Mechanics in Cell Motility
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C., & Plastino, J. (2014) · Physiological Reviews · doi:10.1152/physrev.00018.2013 · Cited by 1,457
Cellular Control of Actin Nucleation
Welch, M. D., & Mullins, R. D. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.040202.112133 · Cited by 517
Microscopic analysis of polymerization dynamics with individual actin filaments
Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T., & Ishiwata, S. (2002) · Nature Cell Biology · doi:10.1038/ncb841 · Cited by 420
Formin proteins: a domain-based approach
Higgs, H. N. (2005) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2005.04.014 · Cited by 386
Kovar, D. R., Kuhn, J. R., Tichy, A. L., & Pollard, T. D. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200211078 · Cited by 337
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Metadata
- Authors
- Amann, K. J., & Pollard, T. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-12-11
- Volume
- 98
- Issue
- 26
- Pages
- 15009-15013
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2108262896
- Cited by
- 263
- References
- 26
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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