Cited by 420Cellular Mechanics and InteractionsMicrotubule and mitosis dynamicsAdvanced Fluorescence Microscopy Techniques

Microscopic analysis of polymerization dynamics with individual actin filaments

Fujiwara, Ikuko, Takahashi, Shin, Tadakuma, Hisashi, Funatsu, Takashi, Ishiwata, Shin'ichi·2002·Nature Cell Biology

Abstract

The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

Integrity signals

Registry

Crossref · 10.1038/ncb841

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T., & Ishiwata, S.
Affiliations
Waseda University (JP)
Corresponding author
Ishiwata, Shin'ichi
Venue
Nature Cell Biology
Published
2002-08-19
Volume
4
Issue
9
Pages
666-673
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
OpenAlex
W2117223239
Cited by
420
References
30
Citations by year
2026: 6 · 2025: 8 · 2024: 1 · 2023: 4 · 2022: 7 · 2021: 9 · 2020: 12 · 2019: 11 · 2018: 6 · 2017: 24 · 2016: 28 · 2015: 22 · 2014: 14 · 2013: 15 · 2012: 24
FWCI
6.08
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Waseda University
  • Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
actin polymerization, actin depolymerization, treadmilling, diffusion, polymerization dynamics, steady-state phases
MeSH terms
Actins, Animals, Biopolymers, Diffusion, Kinetics, Microscopy, Fluorescence, Models, Biological, Rabbits, Stochastic Processes, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review