The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
A conditional tissue-specific transgene expression system using inducible GAL4
Open accessAbstract
In Drosophila , the most widely used system for generating spatially restricted transgene expression is based on the yeast GAL4 protein and its target upstream activating sequence (UAS). To permit temporal as well as spatial control over UAS-transgene expression, we have explored the use of a conditional RU486-dependent GAL4 protein (GeneSwitch) in Drosophila . By using cloned promoter fragments of the embryonic lethal abnormal vision gene or the myosin heavy chain gene, we have expressed GeneSwitch specifically in neurons or muscles and show that its transcriptional activity within the target tissues depends on the presence of the activator RU486 (mifepristone). We used available UAS-reporter lines to demonstrate RU486-dependent tissue-specific transgene expression in larvae. Reporter protein expression could be detected 5 h after systemic application of RU486 by either feeding or “larval bathing.” Transgene expression levels were dose-dependent on RU486 concentration in larval food, with low background expression in the absence of RU486. By using genetically altered ion channels as reporters, we were able to change the physiological properties of larval bodywall muscles in an RU486-dependent fashion. We demonstrate here the applicability of GeneSwitch for conditional tissue-specific expression in Drosophila , and we provide tools to control pre- and postsynaptic expression of transgenes at the larval neuromuscular junction during postembryonic life.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
Brand, A. H., & Perrimon, N. (1993) · Development · doi:10.1242/dev.118.2.401 · Cited by 9,612
Genetic Transformation of Drosophila with Transposable Element Vectors
Rubin, G. M., & Spradling, A. C. (1982) · Science · doi:10.1126/science.6289436 · Cited by 3,014
Transcriptional Activation by Tetracyclines in Mammalian Cells
Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H. (1995) · Science · doi:10.1126/science.7792603 · Cited by 2,488
The embryonic development of Drosophila melanogaster
(1938) · Protoplasma · doi:10.1007/bf01613769 · Cited by 2,142
Luo, L., Liao, Y. J., Jan, L. Y., & Jan, Y. N. (1994) · Genes & Development · doi:10.1101/gad.8.15.1787 · Cited by 983
Sweeney, S. T., Broadie, K., Keane, J., Niemann, H., & O'Kane, C. J. (1995) · Neuron · doi:10.1016/0896-6273(95)90290-2 · Cited by 938
Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter
Li, X., Zhao, X., Fang, Y., Jiang, X., Duong, T., Fan, C., Huang, C.-C., & Kain, S. R. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.52.34970 · Cited by 846
Cited by
Sir2 mediates longevity in the fly through a pathway related to calorie restriction
Rogina, B., & Helfand, S. L. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404184101 · Cited by 1,361
Refinement of Tools for Targeted Gene Expression in Drosophila
Pfeiffer, B. D., Ngo, T.-T. B., Hibbard, K. L., Murphy, C., Jenett, A., Truman, J. W., & Rubin, G. M. (2010) · Genetics · doi:10.1534/genetics.110.119917 · Cited by 1,153
The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.
Zhang, K., Donnelly, C. J., Haeusler, A. R., Grima, J. C., Machamer, J. B., Steinwald, P., Daley, E. L., Miller, S. J., Cunningham, K. M., Vidensky, S., et al. (2015) · Nature · doi:10.1038/nature14973 · Cited by 982
C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins
Mizielinska, S., Grönke, S., Niccoli, T., Ridler, C. E., Clayton, E. L., Devoy, A., Moens, T., Norona, F. E., Woollacott, I. O. C., Pietrzyk, J., et al. (2014) · Science · doi:10.1126/science.1256800 · Cited by 740
Luan, H., Peabody, N. C., Vinson, C. R., & White, B. H. (2006) · Neuron · doi:10.1016/j.neuron.2006.08.028 · Cited by 500
Self-citation
Metadata
- Authors
- Osterwalder, T., Yoon, K. S., White, B. H., & Keshishian, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-10-23
- Volume
- 98
- Issue
- 22
- Pages
- 12596-12601
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2056558785
- Cited by
- 773
- References
- 35
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Osterwalder, T., Yoon, K. S., White, B. H., & Keshishian, H. (2001). A conditional tissue-specific transgene expression system using inducible GAL4. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.221303298
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