The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein
Open accessAbstract
Recently we purified and identified a previously uncharacterized transcription factor from rat liver binding to the carbohydrate responsive element of the L-type pyruvate kinase (L-PK) gene. This factor was named carbohydrate responsive element binding protein (ChREBP). ChREBP, essential for L-PK gene transcription, is activated by high glucose and inhibited by cAMP. Here, we demonstrated that ( i ) nuclear localization signal and basic helix-loop-helix/leucine-zipper domains of ChREBP were essential for the transcription, and ( ii ) these domains were the targets of regulation by cAMP and glucose. Among three cAMP-dependent protein kinase phosphorylation sites, Ser 196 and Thr 666 were the target sites. Phosphorylation of the former resulted in inactivation of nuclear import, and that of the latter resulted in loss of the DNA-binding activity and L-PK transcription. On the other hand, glucose activated the nuclear import by dephosphorylation of Ser 196 in the cytoplasm and also stimulated the DNA-binding activity by dephosphorylation of Thr 666 in the nucleus. These results thus reveal mechanisms for regulation of ChREBP and the L-PK transcription by excess carbohydrate and cAMP.
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A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver
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Self-citationRegulation of protein transport to the nucleus: central role of phosphorylation
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Proteins binding to the liver-specific pyruvate kinase gene promoter
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Glucose Regulation of Mouse S14 Gene Expression in Hepatocytes
Koo, S., & Towle, H. C. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.7.5200 · Cited by 77
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Self-citationHasegawa, J.-I., Osatomi, K., Wu, R. F., & Uyeda, K. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.2.1100 · Cited by 54
Self-citationTranscriptional Activities of the Myc and Max Proteins in Mammalian Cells
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Metadata
- Authors
- Kawaguchi, T., Takenoshita, M., Kabashima, T., & Uyeda, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-11-06
- Volume
- 98
- Issue
- 24
- Pages
- 13710-13715
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2083819344
- Cited by
- 373
- References
- 10
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Kawaguchi, T., Takenoshita, M., Kabashima, T., & Uyeda, K. (2001). Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.231370798
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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