The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
RNA-binding protein Musashi family: Roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation
Open accessAbstract
Homologues of the Musashi family of RNA-binding proteins are evolutionarily conserved across species. In mammals, two members of this family, Musashi1 (Msi1) and Musashi2 (Msi2), are strongly coexpressed in neural precursor cells, including CNS stem cells. To address the in vivo roles of msi in neural development, we generated mice with a targeted disruption of the gene encoding Msi1. Homozygous newborn mice frequently developed obstructive hydrocephalus with aberrant proliferation of ependymal cells in a restricted area surrounding the Sylvius aqueduct. These observations indicate a vital role for msi1 in the normal development of this subpopulation of ependymal cells, which has been speculated to be a source of postnatal CNS stem cells. On the other hand, histological examination and an in vitro neurosphere assay showed that neither the embryonic CNS development nor the self-renewal activity of CNS stem cells in embryonic forebrains appeared to be affected by the disruption of msi1 , but the diversity of the cell types produced by the stem cells was moderately reduced by the msi1 deficiency. Therefore, we performed antisense ablation experiments to target both msi1 and msi2 in embryonic neural precursor cells. Administration of the antisense peptide-nucleotides, which were designed to specifically down-regulate msi2 expression, to msi1 −/− CNS stem cell cultures drastically suppressed the formation of neurospheres in a dose-dependent manner. Antisense-treated msi1 −/− CNS stem cells showed a reduced proliferative activity. These data suggest that msi1 and msi2 are cooperatively involved in the proliferation and maintenance of CNS stem cell populations.
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Cites
Subventricular zone astrocytes are neural stem cells in the adult mammalian brain
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Mouse-Musashi-1, a Neural RNA-Binding Protein Highly Enriched in the Mammalian CNS Stem Cell
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Self-citationAsymmetric Localization of a Mammalian Numb Homolog during Mouse Cortical Neurogenesis
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Self-citationStem cells in the adult mammalian central nervous system
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Musashi: a translational regulator of cell fate
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Self-citationThe bHLH Gene Hes1 as a Repressor of the Neuronal Commitment of CNS Stem Cells
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Identification of a putative intestinal stem cell and early lineage marker; musashi-1
Potten, C. S., Booth, C., Tudor, G. L., Booth, D., Brady, G., Hurley, P., Ashton, G., Clarke, R., Sakakibara, S.-I., & Okano, H. (2003) · Differentiation · doi:10.1046/j.1432-0436.2003.700603.x · Cited by 462
Self-citationMusashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia
Kharas, M. G., Lengner, C. J., Al-Shahrour, F., Bullinger, L., Ball, B., Zaidi, S., Morgan, K., Tam, W., Paktinat, M., Okabe, R., et al. (2010) · Nature Medicine · doi:10.1038/nm.2187 · Cited by 379
The RNA-binding protein HuD regulates neuronal cell identity and maturation
Akamatsu, W., Fujihara, H., Mitsuhashi, T., Yano, M., Shibata, S., Hayakawa, Y., Okano, H. J., Sakakibara, S.-I., Takano, H., Takano, T., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407523102 · Cited by 222
Self-citationCnidarians and the evolutionary origin of the nervous system
Watanabe, H., Fujisawa, T., & Holstein, T. W. (2009) · Development, Growth & Differentiation · doi:10.1111/j.1440-169x.2009.01103.x · Cited by 206
Nova2 Regulates Neuronal Migration through an RNA Switch in Disabled-1 Signaling
Yano, M., Hayakawa-Yano, Y., Mele, A., & Darnell, R. B. (2010) · Neuron · doi:10.1016/j.neuron.2010.05.007 · Cited by 176
Metadata
- Authors
- Sakakibara, S.-I., Nakamura, Y., Yoshida, T., Shibata, S., Koike, M., Takano, H., Ueda, S., Uchiyama, Y., Noda, T., & Okano, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-10-29
- Volume
- 99
- Issue
- 23
- Pages
- 15194-15199
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2018657730
- Cited by
- 354
- References
- 20
- FWCI
- 2.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Sakakibara, S.-I., Nakamura, Y., Yoshida, T., Shibata, S., Koike, M., Takano, H., Ueda, S., Uchiyama, Y., Noda, T., & Okano, H. (2002). RNA-binding protein Musashi family: Roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.232087499
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