The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana
Open accessAbstract
The plant hormone abscisic acid (ABA), synthesized in response to water-deficit stress, induces stomatal closure via activation of complex signaling cascades. Recent work has established that nitric oxide (NO) is a key signaling molecule mediating ABA-induced stomatal closure. However, the biosynthetic origin of NO in guard cells has not yet been resolved. Here, we provide pharmacological, physiological, and genetic evidence that NO synthesis in Arabidopsis guard cells is mediated by the enzyme nitrate reductase (NR). Guard cells of wild-type Arabidopsis generate NO in response to treatment with ABA and nitrite, a substrate for NR. Moreover, NR-mediated NO synthesis is required for ABA-induced stomatal closure. However, in the NR double mutant, nia1 , nia2 that has diminished NR activity, guard cells do not synthesize NO nor do the stomata close in response to ABA or nitrite, although stomatal opening is still inhibited by ABA. Furthermore, by using the ABA-insensitive (ABI) abi1–1 and abi2–1 mutants, we show that the ABI1 and ABI2 protein phosphatases are downstream of NO in the ABA signal-transduction cascade. These data demonstrate a previously uncharacterized signaling role for NR, that of mediating ABA-induced NO synthesis in Arabidopsis guard cells.
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Cites
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Pei, Z.-M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G. J., Grill, E., & Schroeder, J. I. (2000) · Nature · doi:10.1038/35021067 · Cited by 2,109
Nitric oxide functions as a signal in plant disease resistance
Delledonne, M., Xia, Y., Dixon, R. A., & Lamb, C. (1998) · Nature · doi:10.1038/29087 · Cited by 1,857
Hydrogen peroxide and nitric oxide as signalling molecules in plants
Neill, S. J. (2002) · Journal of Experimental Botany · doi:10.1093/jexbot/53.372.1237 · Cited by 1,225
Self-citationG UARD C ELL S IGNAL T RANSDUCTION
Schroeder, J. I., Allen, G. J., Hugouvieux, V., Kwak, J. M., & Waner, D. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.627 · Cited by 1,153
Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro
Rockel, P., Strube, F., Rockel, A., Wildt, J., & Kaiser, W. M. (2002) · Journal of Experimental Botany · doi:10.1093/jexbot/53.366.103 · Cited by 797
Leung, J., Merlot, S., & Giraudat, J. (1997) · The Plant Cell · doi:10.1105/tpc.9.5.759 · Cited by 755
Nitric oxide: comparative synthesis and signaling in animal and plant cells
Wendehenne, D., Pugin, A., Klessig, D. F., & Durner, J. (2001) · Trends in Plant Science · doi:10.1016/s1360-1385(01)01893-3 · Cited by 574
Murata, Y., Pei, Z.-M., Mori, I. C., & Schroeder, J. (2001) · The Plant Cell · doi:10.1105/tpc.010210 · Cited by 572
Yamasaki, H., & Sakihama, Y. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01203-5 · Cited by 548
Cited by
Identification of a Plant Nitric Oxide Synthase Gene Involved in Hormonal Signaling
Guo, F.-Q., Okamoto, M., & Crawford, N. M. (2003) · Science · doi:10.1126/science.1086770 · Cited by 889
Nitric oxide signalling in plants
Neill, S. J., Desikan, R., & Hancock, J. T. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00804.x · Cited by 874
Self-citationSLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling
Vahisalu, T., Kollist, H., Wang, Y.-F., Nishimura, N., Chan, W.-Y., Valerio, G., Lamminmäki, A., Brosché, M., Moldau, H., Desikan, R., et al. (2008) · Nature · doi:10.1038/nature06608 · Cited by 853
Self-citationZhang, Y., Zhu, H., Zhang, Q., Li, M., Yan, M., Wang, R., Wang, L., Welti, R., Zhang, W., & Wang, X. (2009) · The Plant Cell · doi:10.1105/tpc.108.062992 · Cited by 580
Relay and control of abscisic acid signaling
Himmelbach, A., Yang, Y., & Grill, E. (2003) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(03)00090-6 · Cited by 438
Metadata
- Authors
- Desikan, R., Griffiths, R., Hancock, J., & Neill, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-21
- Volume
- 99
- Issue
- 25
- Pages
- 16314-16318
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1990292989
- Cited by
- 710
- References
- 26
- FWCI
- 29.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Desikan, R., Griffiths, R., Hancock, J., & Neill, S. (2002). A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.252461999
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