The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Directed evolution of a genetic circuit
Open accessAbstract
The construction of artificial networks of transcriptional control elements in living cells represents a new frontier for biological engineering. However, biological circuit engineers will have to confront their inability to predict the precise behavior of even the most simple synthetic networks, a serious shortcoming and challenge for the design and construction of more sophisticated genetic circuitry in the future. We propose a combined rational and evolutionary design strategy for constructing genetic regulatory circuits, an approach that allows the engineer to fine-tune the biochemical parameters of the networks experimentally in vivo . By applying directed evolution to genes comprising a simple genetic circuit, we demonstrate that a nonfunctional circuit containing improperly matched components can evolve rapidly into a functional one. In the process, we generated a library of genetic devices with a range of behaviors that can be used to construct more complex circuits.
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Citations
Cites
Quantitative model for gene regulation by lambda phage repressor.
Ackers, G. K., Johnson, A. D., & Shea, M. A. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.4.1129 · Cited by 590
Cited by
Automated design of synthetic ribosome binding sites to control protein expression
Salis, H. M., Mirsky, E. A., & Voigt, C. A. (2009) · Nature Biotechnology · doi:10.1038/nbt.1568 · Cited by 1,844
The second wave of synthetic biology: from modules to systems
Purnick, P. E. M., & Weiss, R. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2698 · Cited by 1,181
Self-citationExploring protein fitness landscapes by directed evolution
Romero, P. A., & Arnold, F. H. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2805 · Cited by 1,161
Self-citationSynthetic biology: new engineering rules for an emerging discipline
Andrianantoandro, E., Basu, S., Karig, D. K., & Weiss, R. (2006) · Molecular Systems Biology · doi:10.1038/msb4100073 · Cited by 1,081
Self-citationGenetic circuit design automation
Nielsen, A. A. K., Der, B. S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E. A., Ross, D., Densmore, D., & Voigt, C. A. (2016) · Science · doi:10.1126/science.aac7341 · Cited by 1,020
Metadata
- Authors
- Yokobayashi, Y., Weiss, R., & Arnold, F. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-25
- Volume
- 99
- Issue
- 26
- Pages
- 16587-16591
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1979159575
- Cited by
- 455
- References
- 1
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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