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THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEIN
Open accessAbstract
The nerve growth factor protein was purified over 100-fold from adult mouse salivary glands. The first step was a gel filtration on Sephadex G-100 at pH 7.5 of the aqueous gland extract. After gel filtration, most of the NGF activity was eluted in the 80,000-90,000-molecular-weight region (G-100 pool). The G-100 pool was dialyzed at pH 5.0 and fractionated by CM52 cellulose chromatography at pH 5.0. Recovery from CM52 cellulose columns was quantitative for protein and ranged 80-100 per cent for the nerve growth factor activity; the latter was almost completely carried by a protein which did not show any heterogeneity when examined by several analytical tests. The purified nerve growth factor showed an S 20, w = 2.43, a D 20, w = 7.30 and a 30,000 molecular weight. The over-all recovery was about 45 per cent.
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Citations
Cited by
Purification of a new neurotrophic factor from mammalian brain.
Barde, Y. A., Edgar, D., & Thoenen, H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01207.x · Cited by 1,822
Trophic Mechanisms in the Peripheral Nervous System
Varon, S. S., & Bunge, R. P. (1978) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.01.030178.001551 · Cited by 326
Topical treatment with nerve growth factor for neurotrophic keratitis
Bonini, S. (2000) · Ophthalmology · doi:10.1016/s0161-6420(00)00163-9 · Cited by 320
Hartikka, J., & Hefti, F. (1988) · The Journal of Neuroscience · doi:10.1523/jneurosci.08-08-02967.1988 · Cited by 317
Satoh, T., Sakai, N., Enokido, Y., Uchiyama, Y., & Hatanaka, H. (1996) · Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a021447 · Cited by 146
Metadata
- Authors
- Bocchini, V., & Angeletti, P. U.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1969-10
- Volume
- 64
- Issue
- 2
- Pages
- 787-794
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2027243420
- Cited by
- 906
- References
- 0
- FWCI
- 5.09
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEIN. https://app.cochat.ai/papers/10.1073/pnas.64.2.787
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- Bocchini, V., & Angeletti, P. U. (1969). THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEIN. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.64.2.787
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
