EVIDENCE FOR A SINGLE-STRANDED ADENOVIRUS-ASSOCIATED VIRUS GENOME: FORMATION OF A DNA DENSITY HYBRID ON RELEASE OF VIRAL DNA
Open accessAbstract
Extracted adenovirus-associated virus DNA is known to be double-stranded, and, therefore, it has been assumed that these virus particles contain a double-stranded genome. Recent findings, however, have suggested that the DNA in virus particles is equivalent to only half the molecular weight of extracted molecules. A density analysis of DNA extracted from a mixture of virus particles containing either bromodeoxyuridine-substituted or unsubstituted DNA shows that virions contain single-stranded DNA which, when released, forms duplex structures. A similar circumstance is as yet unknown among other viruses.
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The maximum packaging capacity of members of the Parvoviridae family is approximately 4.5-5.5 kb.
“This suggests that the maximum packaging capacity of members of the Parvoviridae family is approximately 4.5-5.5 kb.”
From Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids (2016) · cites this paper as [8]
The Introduction reports that AAV-1 DNA in a particle should not exceed 1.7 × 10⁶ daltons, and the paper describes one DNA strand per AAV virion. These passages support an approximate genome size for AAV, but they do not establish a 4.5–5.5 kb maximum packaging capacity for the Parvoviridae family. No page number is supplied in our copy. (LLM, conf=0.84)
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- 1 Rose, J. A., M. D. Hoggan, and A. J. Shatkin, these PRocEEDINGs, 56, 86 (1966).
- 3 Rose, J. A., M. D. Hoggan, F. Koczot, and A. J. Shatkin, J. Virology, 2, 999 (1968).
- 2 Parks, W. P., M. Green, M. Piña, and J. L. Melnick, J. Virology, 1, 980 (1967).
- 4 Mayor, H. D., and J. L. Melnick, Nature, 210, 331 (1966).
- Crawford, L. V., E. A. C. Follett, M. G. Burdon, and D. J. McGeoch, J. Gen. Virol., 4, 37 (1969).
- 7 Hoggan, M. D., N. R. Blacklow, and W. P. Rowe, these PRocEEDINGs, 55, 1467 (1966).
- 9 Burgi, E., and A. D. Hershey, Biophys. J., 3, 309 (1963).
- 8 Patterson, M. S., and R. C. Greene, Analyt. Chem., 37, 854 (1965).
- 10 Studier, F. W., J. Mol. Biol., 11, 373 (1965).
- 12 Rolfe, R., J. Mol. Biol., 4, 22 (1962).
- 11 Baldwin, R. L., and E. M. Shooter, J. Mol. Biol., 7, 511 (1963).
- 13 Hershey, A. D., E. Burgi, and L. Ingraham, these PRoCEEDINGs, 49, 748 (1963).
- 15 Piña, M., and M. Green, these PRoCEEDINGs, 54, 547 (1965).
- 14 Mac Hattie, L. A., and C. A. Thomas, Science, 144, 1142 (1964).
- 16 Atchison, R. W., B. Casto, and W. Mc D). Hammon, Science, 149, 754 (1965).
- 18 Smith, K. O., W. D. Gehle, and J. F. Thiel, J. Immunol., 97, 754 (1966).
- 17 Hoggan, M. D., A. J. Shatkin, N. R. Blacklow, F. Koczot, and J. A. Rose, J. Virology, 2, 850 (1968).
- 19 Parks, W. P., J. L. Melnick, R. Rongey, and H. D. Mayor, J. Virol., 1, 171 (1967).
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- Authors
- Rose, J. A., Berns, K. I., Hoggan, M. D., & Koczot, F. J.
- Affiliations
- National Institutes of Health (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1969-11
- Volume
- 64
- Issue
- 3
- Pages
- 863-869
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC223314
- OpenAlex
- W2169910348
- Cited by
- 179
- References
- 0
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 3 · 2021: 6 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 5 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 7.51
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- single-stranded DNA, viral genome, DNA duplex formation, virus particles
- MeSH terms
- Adenoviridae, Bromodeoxyuridine, Centrifugation, Density Gradient, Centrifugation, Zonal, DNA, Viral, Hybridization, Genetic, Satellite Viruses, Specific Gravity
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