Cited by 111Parvovirus B19 Infection StudiesVirus-based gene therapy researchVirology and Viral Diseases

Characterization of adenovirus-associated virus-induced polypeptides in KB cells

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Buller, R M, Rose, J A·1978·Journal of Virology

Abstract

Electrophoretic analysis of KB cells coinfected with adenovirus-associated virus (AAV) type 2, a defective parvovirus, and adenovirus type 5 (as helper) have revealed the synthesis in vivo of at least five AAV-specific polypeptides. The three largest polypeptides, with molecular weights of 90,700, 71,600, and 60,000 comigrated in polyacrylamide gels with the three AAV structural polypeptides. The remaining two polypeptides had molecular weights of 24,900 and 15,800. The concentrations of the AAV-induced polypeptides relative to one another remained approximately constant during the infectious cycle, and the structural components were present in proportions similar to those found in purified virions. As determined by pulse-chase experiments, all polypeptides were generated at the level of protein synthesis and not by posttranslational proteolytic processing. Although inhibitors of proteolytic enzymes failed to influence the pattern of AAV-induced polypeptides, and amino acid analog, L-canavanine, blocked the appearances of both the major structural polypeptide (60,000 daltons) and the larger nonstructural polypeptide (24,900 daltons). Taken in conjunction with pulse-chase data, this result supports a model whereby the major virion polypeptide is produced by proteolytic cleavage of the nascent polypeptide chain.

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Crossref · 10.1128/jvi.25.1.331-338.1978

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  • The AAV capsid is composed of 60 subunits of overlapping VP1:VP2:VP3 in an approximate ratio of 1:1:10, assembled with T=1 icosahedral symmetry and a diameter of approximately 260 Å.

    “The AAV capsid is composed of 60 subunits of the overlapping VP1:VP2:VP3, in an approximate ratio of 1:1:10, assembled with T = 1 icosahedral symmetry and a diameter of approximately 260 Å [13,14].”

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Authors
Buller, R. M., & Rose, J. A.
Venue
Journal of Virology
Published
1978-01
Volume
25
Issue
1
Pages
331-338
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
OpenAlex
W1517847098
Cited by
111
References
24
Citations by year
2025: 2 · 2024: 6 · 2023: 11 · 2022: 2 · 2021: 8 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 2
FWCI
4.92
Type
journal article
Language
en
Open access
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
KB cells, proteolytic processing, L-canavanine, pulse-chase labeling, viral protein synthesis, infectious cycle
MeSH terms
Adenoviruses, Human, Canavanine, Cell Line, Humans, Molecular Weight, Parvoviridae, Peptide Biosynthesis, Phenylmethylsulfonyl Fluoride, Satellite Viruses, Tosyllysine Chloromethyl Ketone, Tosylphenylalanyl Chloromethyl Ketone, Viral Proteins, Virus Replication

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