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Dissociation of Mammalian Polyribosomes into Subunits by Puromycin
Open accessAbstract
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37°C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.
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Cites
Amino acid metabolism in Mammalian cell cultures
Eagle, H. (1959) · Science · doi:10.1126/science.130.3373.432 · Cited by 4,011
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A transfer-RNA-derived small RNA regulates ribosome biogenesis
Kim, H. K., Fuchs, G., Wang, S., Wei, W., Zhang, Y., Park, H., Roy-Chaudhuri, B., Li, P., Xu, J., Chu, K., et al. (2017) · Nature · doi:10.1038/nature25005 · Cited by 574
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Gerashchenko, M. V., & Gladyshev, V. N. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku671 · Cited by 262
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Metadata
- Authors
- Blobel, G., & Sabatini, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1971-02
- Volume
- 68
- Issue
- 2
- Pages
- 390-394
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1980600234
- Cited by
- 437
- References
- 1
- FWCI
- 13.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Blobel, G., & Sabatini, D. (1971). Dissociation of Mammalian Polyribosomes into Subunits by Puromycin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.68.2.390
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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