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Cited by 516

The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.

A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites Cells

Open access
Lee, Se Yong, Mendecki, Jozef, Brawerman, George·1971·Proceedings of the National Academy of Sciences

Abstract

The rapidly-labeled polyribosomal RNA component from mouse sarcoma 180 cells is retained on nitrocellulose (Millipore) membrane-filters at high ionic strength. This property is due to the presence of a polynucleotide sequence rich in adenylic acid that resists both T 1 and pancreatic RNase digestion. The resistant material shows sedimentation characteristics close to those of transfer RNA. The RNA molecules that contain this material can be separated from the rest of the polysomal RNA by differential phenol extraction with neutral and alkaline Tris buffers. Synthetic poly(A) exhibits the same behavior as the rapidly-labeled polysomal RNA with respect to Millipore binding and phenol fractionation. The characteristics of the rapidly-labeled polysomal RNA component permit its isolation free of ribosomal RNA.

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Crossref · 10.1073/pnas.68.6.1331

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Metadata

Authors
Lee, S. Y., Mendecki, J., & Brawerman, G.
Venue
Proceedings of the National Academy of Sciences
Published
1971-06
Volume
68
Issue
6
Pages
1331-1335
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1977208035
Cited by
516
References
14
FWCI
41.83
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites Cells. https://app.cochat.ai/papers/10.1073/pnas.68.6.1331
The paper
Lee, S. Y., Mendecki, J., & Brawerman, G. (1971). A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites Cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.68.6.1331

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.