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Cited by 296

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.

Open access
Ramos, S, Schuldiner, S, Kaback, H R·1976·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.73.6.1892
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Abstract

Membrane vesicles isolated from E. coli generate a trans-membrane proton gradient of 2 pH units under appropriate conditions when assayed by flow dialysis. Using the distribution of weak acids to measure the proton gradient (deltapH) and the distribution of the lipophilic cation triphenyl-methylphosphonium to measure the electrical potential across the membrane (delta psi), the vesicles are shown to generate an electrochemical proton gradient (deltamuH+) of approximately-180 mV at pH 5.5 in the presence of ascorbate and phenazine methosulfate, the major component of which is a deltapH of about -110mV. As external pH is increased, deltapH decreases, reaching 0 at pH 7.5 and above, while delta psi remains at about-75 mV and internal pH remains at pH 7.5. Moreover, the ability of various electron donors to drive transport is correlated with their ability to generate deltamuH+. In addition, deltapH and delta psi can be varied reciprocally in the presence of valinomycin and nigericin. These data and others (manuscript in preparation) provide convincing support for the role of chemiosmotic phenomena in active transport.

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Metadata

Authors
Ramos, S., Schuldiner, S., & Kaback, H. R.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
1976-06
Volume
73
Issue
6
Pages
1892-1896
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.73.6.1892
OpenAlex
W2064033773
Cited by
296
References
17
FWCI
16.25
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. https://app.cochat.ai/papers/10.1073/pnas.73.6.1892
The paper
Ramos, S., Schuldiner, S., & Kaback, H. R. (1976). The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.6.1892

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.