The DOI matches its Crossref record.
Lymphocyte stimulation: a rapid multiparameter analysis.
Open accessAbstract
Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.
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Cited by
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Metadata
- Authors
- Darzynkiewicz, Z., Traganos, F., Sharpless, T., & Melamed, M. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-08
- Volume
- 73
- Issue
- 8
- Pages
- 2881-2884
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2000356987
- Cited by
- 347
- References
- 0
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Lymphocyte stimulation: a rapid multiparameter analysis. https://app.cochat.ai/papers/10.1073/pnas.73.8.2881
- The paper
- Darzynkiewicz, Z., Traganos, F., Sharpless, T., & Melamed, M. R. (1976). Lymphocyte stimulation: a rapid multiparameter analysis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.8.2881
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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