The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Open accessAbstract
The sperm chromatin structure assay (SCSA) was used to measure over 500 human semen samples from two independent studies: Study I, 402 samples from 165 presumably fertile couples wishing to achieve pregnancy over 12 menstrual cycles; Study II, samples from 115 patients seeking fertility counselling. The SCSA measures susceptibility to DNA denaturation in situ in spermatozoa exposed to acid for 30 s, followed by acridine orange staining. SCSA data from the male partners of 73 couples (group 1) achieving pregnancy during months 1-3 of Study I were used as the standard of 'sperm chromatin compatible with high fertility' and were significantly different from those of 40 couples (group 3) achieving pregnancy in months 4-12 (P < 0.01) and those of male partners of 31 couples (group 4) not achieving pregnancy (P < 0.001). Group 2 contained couples who had a miscarriage. SCSA values for Study II were almost twice that of the Study I fertility standards. Within-couple repeatability tended to be less for group 3 than for groups 1, 2 or 4. Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMP alpha t) were the best predictor for whether a couple would not achieve pregnancy. Some 84% of males in group 1 had COMP alpha t < 15%, while no couples achieved pregnancy in group 1 with > or = 30% COMP alpha t, a threshold level considered not compatible with good fertility. Using selected cut-off values for chromatin integrity, the SCSA data predicted seven of 18 miscarriages (39%).
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Cites
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Self-citationEstimates of human fertility and pregnancy loss
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Cited by
EVENSON, D. P., LARSON, K. L., & JOST, L. K. (2002) · Journal of Andrology · doi:10.1002/j.1939-4640.2002.tb02599.x · Cited by 1,072
Self-citationThe effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis
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Oxidative stress, DNA damage and apoptosis in male infertility: A clinical approach
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Metadata
- Authors
- Evenson, D. P.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-04-01
- Volume
- 14
- Issue
- 4
- Pages
- 1039-1049
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2109269254
- Cited by
- 1,121
- References
- 29
- FWCI
- 22.65
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Evenson, D. P. (1999). Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction. https://doi.org/10.1093/humrep/14.4.1039
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
