The DOI matches its Crossref record.
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
Open accessAbstract
We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.
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Citations
Cited by
Yoshida, M., Miyoshi, I., & Hinuma, Y. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.6.2031 · Cited by 2,169
Twenty-Five Years of Quantitative PCR for Gene Expression Analysis
VanGuilder, H. D., Vrana, K. E., & Freeman, W. M. (2008) · BioTechniques · doi:10.2144/000112776 · Cited by 1,246
In vivo sequence requirements of the SV40 early promoter region
Benoist, C., & Chambon, P. (1981) · Nature · doi:10.1038/290304a0 · Cited by 1,074
Wahl, G. M., Padgett, R. A., & Stark, G. R. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86945-4 · Cited by 451
Self-citationUnintended effects and their detection in genetically modified crops
Cellini, F., Chesson, A., Colquhoun, I., Constable, A., Davies, H. V., Engel, K. H., Gatehouse, A. M. R., Kärenlampi, S., Kok, E. J., Leguay, J.-J., et al. (2004) · Food and Chemical Toxicology · doi:10.1016/j.fct.2004.02.003 · Cited by 397
Metadata
- Authors
- Alwine, J. C., Kemp, D. J., & Stark, G. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1977-12
- Volume
- 74
- Issue
- 12
- Pages
- 5350-5354
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2016613744
- Cited by
- 2,236
- References
- 0
- FWCI
- 41.44
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. https://app.cochat.ai/papers/10.1073/pnas.74.12.5350
- The paper
- Alwine, J. C., Kemp, D. J., & Stark, G. R. (1977). Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.74.12.5350
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
