The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy
Open accessAbstract
Electron microscopy and indirect immunofluorescence microscopy using monospecific tubulin antibodies were performed in parallel on glutaraldehyde-fixed tissue culture cells without osmium fixation. In order to reduce the excess aldehyde groups of the strongly crosslinked cellular matrix, which normally interfere with subsequent immunofluorescence microscopy, a mild NaBH 4 treatment was introduced during or after the dehydration steps. Cells processed through the NaBH 4 step show, in transmission electron microscopy, normal cytoplasmic microtubules approximately 250 Å in diameter. When such cells are subjected to indirect immunofluorescence microscopy using monospecific tubulin antibody they reveal a complex system of unbroken, fine, fluorescent fibers traversing the cytoplasm between the perinuclear space and the plasma membrane. Thin sections of cells processed through the indirect immunofluorescence procedure show antibody-decorated microtubules with a diameter of approximately 600 Å. This decoration is not obtained when non-immune IgGs are used instead of monospecific antitubulin IgGs. Thus, a direct comparison of cytoplasmic microtubules in glutaraldehyde-fixed cells by both electron microscopy and immunofluorescence microscopy can be obtained.
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Metadata
- Authors
- Weber, K., Rathke, P. C., & Osborn, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-04
- Volume
- 75
- Issue
- 4
- Pages
- 1820-1824
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2094010512
- Cited by
- 255
- References
- 14
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- green
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