The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Iterative expansion microscopy
Open accessAbstract
We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel–specimen composite by ∼4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded ∼20×. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens ∼4.5 × 4.5, or ∼20×, and enables ∼25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
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Self-citationImmuno-SABER enables highly multiplexed and amplified protein imaging in tissues
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One-step nanoscale expansion microscopy reveals individual protein shapes
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Metadata
- Authors
- Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock, H., Kang, J. S., Asano, S., Suk, H.-J., Pak, N., Tillberg, P. W., Wassie, A. T., Cai, D., & Boyden, E. S.
- Venue
- Nature Methods
- Published
- 2017-04-17
- Volume
- 14
- Issue
- 6
- Pages
- 593-599
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W4232515155
- Cited by
- 386
- References
- 25
- FWCI
- 19.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock, H., Kang, J. S., Asano, S., Suk, H.-J., Pak, N., Tillberg, P. W., Wassie, A. T., Cai, D., & Boyden, E. S. (2017). Iterative expansion microscopy. Nature Methods. https://doi.org/10.1038/nmeth.4261
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